SubtiBank SubtiBank

Description: trigger enzyme: methylphosphotriester-DNA alkyltransferase/ transcriptional activator (AraC family) of the adaA-adaB operon

Name: adaA
Locus: BSU01810BsubCyc
pI: 9.2
MW: 24.0 kDa
Protein length: 211 aaBLASTSequence
Gene length: 633 bpBLASTSequenceSequence + Flanks
Function: adaptive response to alkylative DNA damage
Product: trigger enzyme: methylphosphotriester-DNA alkyltransferase/
transcriptional activator of the adaA-adaB operon
Essential: no
E.C. number:
Synonyms:
Databases: SubtiListKEGGUniProtExpression data browser

Categories containing this gene/protein


DNA repair/ recombination, transcription factors and their control, trigger enzyme, prophage 1

This gene is a member of the following regulons


AdaA regulon

The AdaA regulon


Gene


  • Coordinates on the chromosome (coding sequence): 203,729 -> 204,364
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • AraC family
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

  • adaA-adaB PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • positive control by AdaA PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Reviews

    Mielecki D, Grzesiuk E

    Ada response - a strategy for repair of alkylated DNA in bacteria

    FEMS Microbiol Lett. 2014 Jun;355(1):1-11. doi: 10.1111/1574-6968.12462. Review. PubMed PMID: 24810496; PubMed Central PMCID: PMC4437013.
    Lenhart JS, Schroeder JW, Walsh BW, Simmons LA

    DNA repair and genome maintenance in Bacillus subtilis

    Microbiol Mol Biol Rev. 2012 Sep;76(3):530-64. doi: 10.1128/MMBR.05020-11. Review. PubMed PMID: 22933559; PubMed Central PMCID: PMC3429619.

    Original publications

    Leyn SA, Kazanov MD, Sernova NV, Ermakova EO, Novichkov PS, Rodionov DA

    Genomic reconstruction of the transcriptional regulatory network in Bacillus subtilis

    J Bacteriol. 2013 Jun;195(11):2463-73. doi: 10.1128/JB.00140-13. PubMed PMID: 23504016; PubMed Central PMCID: PMC3676070.
    Morohoshi F, Hayashi K, Munkata N

    Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon

    J Bacteriol. 1993 Sep;175(18):6010-7. PubMed PMID: 8376346; PubMed Central PMCID: PMC206683.
    Morohoshi F, Hayashi K, Munakata N

    Bacillus subtilis ada operon encodes two DNA alkyltransferases

    Nucleic Acids Res. 1990 Sep 25;18(18):5473-80. PubMed PMID: 2120677; PubMed Central PMCID: PMC332226.
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