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Categories containing this gene/protein


transcription factors and their control, utilization of amino acids

This gene is a member of the following regulons


The AdeR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 3,276,955 -> 3,278,223
  • Phenotypes of a mutant

  • sporulation defect due to lack of ald expression PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • transcription activation of ald gene expression in the presence of alanine PubMed
  • Protein family

  • PucR family
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

  • phosphorylated on Ser-395 PubMed
  • Cofactors

    Effectors of protein activity

  • L-alanine PubMed
  • Structure

    Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

  • adeR PubMed
  • Sigma factor

    Regulation

  • constitutively expressed PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • MGNA-A631 (yukF::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Lin TH, Wei GT, Su CC, Shaw GC

    AdeR, a PucR-type transcription factor, activates expression of L-alanine dehydrogenase and is required for sporulation of Bacillus subtilis

    J Bacteriol. 2012 Sep;194(18):4995-5001. doi: 10.1128/JB.00778-12. Epub 2012 Jul 13. PubMed PMID: 22797752; PubMed Central PMCID: PMC3430345.
    Soufi B, Kumar C, Gnad F, Mann M, Mijakovic I, Macek B

    Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis

    J Proteome Res. 2010 Jul 2;9(7):3638-46. doi: 10.1021/pr100150w. PubMed PMID: 20509597.
    Siranosian KJ, Ireton K, Grossman AD

    Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis

    J Bacteriol. 1993 Nov;175(21):6789-96. PubMed PMID: 8226620; PubMed Central PMCID: PMC206802.
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