SubtiBank SubtiBank

Categories containing this gene/protein


carbon core metabolism, transcription factors and their control

This gene is a member of the following regulons


The AlsR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 3,711,498 -> 3,712,406
  • Phenotypes of a mutant

  • no acetoin production
  • The protein


    Catalyzed reaction/ biological activity

  • binds the promoter region of the alsS-alsD operon in the presence of acetate, activates transcription
  • Protein family

  • LysR family of transcription factors
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

    Sigma factor

    Regulation

    Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • AMBs3 (spc), available in the Stülke lab
  • 1A978 ( alsR::cat), PubMed, available at BGSC
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Elisabeth Härtig, Braunschweig, Germany Homepage
  • References


    Reviews

    Härtig E(1), Jahn D.

    Regulation of the anaerobic metabolism in Bacillus subtilis.

    Adv Microb Physiol. 2012;61:195-216. doi: 10.1016/B978-0-12-394423-8.00005-6.DOI: 10.1016/B978-0-12-394423-8.00005-6 PMID: 23046954 [Indexed for MEDLINE]

    Original publications

    Zhang X, Zhang R, Bao T, Yang T, Xu M, Li H, Xu Z, Rao Z

    Moderate expression of the transcriptional regulator ALsR enhances acetoin production by Bacillus subtilis

    J Ind Microbiol Biotechnol. 2013 Sep;40(9):1067-76. doi: 10.1007/s10295-013-1303-5. Epub 2013 Jul 9. PubMed PMID: 23836140.
    Frädrich C, Krausze J, Quade N, Heinz D, Jahn D, Härtig E

    Purification, crystallization and preliminary X-ray analysis of the effector domain of AlsR, an LysR-type transcriptional regulator from Bacillus subtilis

    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May 1;69(Pt 5):581-4. doi: 10.1107/S1744309113010725. Epub 2013 Apr 30. PubMed PMID: 23695583; PubMed Central PMCID: PMC3660907.
    de Oliveira RR, Nicholson WL

    The LysR-type transcriptional regulator (LTTR) AlsR indirectly regulates expression of the Bacillus subtilis bdhA gene encoding 2,3-butanediol dehydrogenase

    Appl Microbiol Biotechnol. 2013 Aug;97(16):7307-16. doi: 10.1007/s00253-013-4871-4. Epub 2013 Apr 11. PubMed PMID: 23576037.
    Nicholson WL

    Increased competitive fitness of Bacillus subtilis under nonsporulating conditions via inactivation of pleiotropic regulators AlsR, SigD, and SigW

    Appl Environ Microbiol. 2012 May;78(9):3500-3. doi: 10.1128/AEM.07742-11. Epub 2012 Feb 17. PubMed PMID: 22344650; PubMed Central PMCID: PMC3346490.
    Frädrich C, March A, Fiege K, Hartmann A, Jahn D, Härtig E

    The transcription factor AlsR binds and regulates the promoter of the alsSD operon responsible for acetoin formation in Bacillus subtilis

    J Bacteriol. 2012 Mar;194(5):1100-12. doi: 10.1128/JB.06425-11. Epub 2011 Dec 16. PubMed PMID: 22178965; PubMed Central PMCID: PMC3294794.
    Brown CT, Fishwick LK, Chokshi BM, Cuff MA, Jackson JM 4th, Oglesby T, Rioux AT, Rodriguez E, Stupp GS, Trupp AH, Woollcombe-Clarke JS, Wright TN, Zaragoza WJ, Drew JC, Triplett EW, Nicholson WL

    Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation

    Appl Environ Microbiol. 2011 Oct;77(19):6867-77. doi: 10.1128/AEM.05272-11. Epub 2011 Aug 5. PubMed PMID: 21821766; PubMed Central PMCID: PMC3187084.
    Renna MC, Najimudin N, Winik LR, Zahler SA

    Regulation of the Bacillus subtilis alsS, alsD, and alsR genes involved in post-exponential-phase production of acetoin

    J Bacteriol. 1993 Jun;175(12):3863-75. PubMed PMID: 7685336; PubMed Central PMCID: PMC204803.
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