SubtiBank SubtiBank

Categories containing this gene/protein


utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons


AnsR regulon

The AnsR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,456,990 -> 2,457,340
  • Phenotypes of a mutant

  • the ansR mutation allows glutamate utilization of a rocG gudB mutant PubMed
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • Xre family
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

  • cytoplasm (according to Swiss-Prot)
  • Interactions

    Additional information

    Expression and Regulation


    Operon

  • ansR PubMed
  • Sigma factor

    Regulation

  • induced in the presence of asparagine (AnsR) PubMed
  • Regulatory mechanism

  • AnsR: negative autoregulation PubMed
  • Additional information

    Biological materials


    Mutant

  • GP1152 (del tet) available in the Stülke lab
  • Expression vector

  • expression of native ansR in B. subtilis: pGP873 (in pBQ200), available in Stülke lab
  • lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Flórez LA, Gunka K, Polanía R, Tholen S, Stülke J

    SPABBATS: A pathway-discovery method based on Boolean satisfiability that facilitates the characterization of suppressor mutants

    BMC Syst Biol. 2011 Jan 11;5:5. doi: 10.1186/1752-0509-5-5. PubMed PMID: 21219666; PubMed Central PMCID: PMC3024933.
    Fisher SH, Wray LV Jr

    Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase

    J Bacteriol. 2002 Apr;184(8):2148-54. PubMed PMID: 11914346; PubMed Central PMCID: PMC134974.
    Sun D, Setlow P

    Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase

    J Bacteriol. 1993 May;175(9):2501-6. PubMed PMID: 8478318; PubMed Central PMCID: PMC204550.
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