SubtiBank SubtiBank

Description: alkyl-sulphur catabolism regulator, transcriptional activator of the snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM operon

Name: ascR
Locus: BSU29400BsubCyc
pI: 6.2
MW: 35.0 kDa
Protein length: 308 aaBLASTSequence
Gene length: 924 bpBLASTSequenceSequence + Flanks
Function: regulation of sulphur metabolism
Product: alkyl-sulphur catabolism regulator, transcriptional activator (LysR family)
Essential: no
E.C. number:
Synonyms: ytlI
Databases: SubtiListKEGGUniProtExpression data browser
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Categories containing this gene/protein


biosynthesis/ acquisition of amino acids, sulfur metabolism, transcription factors and their control

This gene is a member of the following regulons


CymR regulon

The AscR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 3,008,938 -> 3,009,864
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • LysR family
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

  • ascR PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • repressed in the presence of cysteine (CymR) PubMed
  • Regulatory mechanism

  • CymR: transcription repression PubMed
  • Additional information

    Biological materials


    Mutant

  • MGNA-A169 (ytlI::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Chan CM, Danchin A, Marlière P, Sekowska A

    Paralogous metabolism: S-alkyl-cysteine degradation in Bacillus subtilis

    Environ Microbiol. 2014 Jan;16(1):101-17. doi: 10.1111/1462-2920.12210. PubMed PMID: 23944997.
    Even S, Burguière P, Auger S, Soutourina O, Danchin A, Martin-Verstraete I

    Global control of cysteine metabolism by CymR in Bacillus subtilis

    J Bacteriol. 2006 Mar;188(6):2184-97. PubMed PMID: 16513748; PubMed Central PMCID: PMC1428143.
    Burguière P, Fert J, Guillouard I, Auger S, Danchin A, Martin-Verstraete I

    Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism

    J Bacteriol. 2005 Sep;187(17):6019-30. PubMed PMID: 16109943; PubMed Central PMCID: PMC1196162.
    Burguière P, Fert J, Guillouard I, Auger S, Danchin A, Martin-Verstraete I

    Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism

    J Bacteriol. 2005 Sep;187(17):6019-30. PubMed PMID: 16109943; PubMed Central PMCID: PMC1196162.
    Erwin KN, Nakano S, Zuber P

    Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx

    J Bacteriol. 2005 Jun;187(12):4042-9. PubMed PMID: 15937167; PubMed Central PMCID: PMC1151713.
    Coppée JY, Auger S, Turlin E, Sekowska A, Le Caer JP, Labas V, Vagner V, Danchin A, Martin-Verstraete I

    Sulfur-limitation-regulated proteins in Bacillus subtilis: a two-dimensional gel electrophoresis study

    Microbiology. 2001 Jun;147(Pt 6):1631-40. PubMed PMID: 11390694.
    Lapidus A, Galleron N, Sorokin A, Ehrlich SD

    Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region

    Microbiology. 1997 Nov;143 ( Pt 11):3431-41. PubMed PMID: 9387221.
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