SubtiBank SubtiBank

Description: two-component response regulator (OmpR family), regulation of bceA-bceB in response to external (bacitracin, plectasin, mersacidin and actagardine) and internal (SdpC, SkfA) antimicrobial peptides

Name: bceR
Locus: BSU30400BsubCyc
pI: 5.0
MW: 26.0 kDa
Protein length: 231 aaBLASTSequence
Gene length: 693 bpBLASTSequenceSequence + Flanks
Function: resistance against toxic peptides
Product: two-component response regulator (OmpR family)
Essential: no
E.C. number:
Synonyms: ytsA
Databases: SubtiListKEGGUniProtExpression data browser

Categories containing this gene/protein


transcription factors and their control, resistance against toxins/ antibiotics, phosphoproteins

This gene is a member of the following regulons


The BceR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 3,113,187 -> 3,113,882
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • OmpR family
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

  • phosphorylated by BceS on an Asp residue
  • Cofactors

    Effectors of protein activity

    Structure

  • 1KGS (DrrD from Thermotoga maritima , 32% identity) PubMed
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Interactions

  • BceS-BceR PubMed
  • Additional information

    Expression and Regulation


    Operon

  • bceR-bceS PubMed
  • Sigma factor

    Regulation

    Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • MGNA-A133 (ytsA::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Reviews

    Piepenbreier H, Fritz G, Gebhard S

    Transporters as information processors in bacterial signalling pathways

    Mol Microbiol. 2017 Apr;104(1):1-15. doi: 10.1111/mmi.13633. Epub 2017 Feb 24. Review. PubMed PMID: 28152228.
    Radeck J, Fritz G, Mascher T

    The cell envelope stress response of Bacillus subtilis: from static signaling devices to dynamic regulatory network

    Curr Genet. 2017 Feb;63(1):79-90. doi: 10.1007/s00294-016-0624-0. Review. PubMed PMID: 27344142.

    Original publications

    Fang C, Nagy-Staroń A, Grafe M, Heermann R, Jung K, Gebhard S, Mascher T

    Insulation and wiring specificity of BceR-like response regulators and their target promoters in Bacillus subtilis

    Mol Microbiol. 2017 Apr;104(1):16-31. doi: 10.1111/mmi.13597. Epub 2017 Jan 27. PubMed PMID: 27997719.
    Höfler C, Heckmann J, Fritsch A, Popp P, Gebhard S, Fritz G, Mascher T

    Cannibalism stress response in Bacillus subtilis

    Microbiology. 2016 Jan;162(1):164-76. doi: 10.1099/mic.0.000176. PubMed PMID: 26364265.
    Fritz G, Dintner S, Treichel NS, Radeck J, Gerland U, Mascher T, Gebhard S

    A New Way of Sensing: Need-Based Activation of Antibiotic Resistance by a Flux-Sensing Mechanism

    MBio. 2015 Jul 21;6(4):e00975. doi: 10.1128/mBio.00975-15. PubMed PMID: 26199330; PubMed Central PMCID: PMC4513084.
    Dintner S, Heermann R, Fang C, Jung K, Gebhard S

    A sensory complex consisting of an ATP-binding cassette transporter and a two-component regulatory system controls bacitracin resistance in Bacillus subtilis

    J Biol Chem. 2014 Oct 3;289(40):27899-910. doi: 10.1074/jbc.M114.596221. PubMed PMID: 25118291; PubMed Central PMCID: PMC4183823.
    Wolf D, Domínguez-Cuevas P, Daniel RA, Mascher T

    Cell envelope stress response in cell wall-deficient L-forms of Bacillus subtilis

    Antimicrob Agents Chemother. 2012 Nov;56(11):5907-15. doi: 10.1128/AAC.00770-12. PubMed PMID: 22964256; PubMed Central PMCID: PMC3486569.
    Coumes-Florens S, Brochier-Armanet C, Guiseppi A, Denizot F, Foglino M

    A new highly conserved antibiotic sensing/resistance pathway in firmicutes involves an ABC transporter interplaying with a signal transduction system

    PLoS One. 2011 Jan 19;6(1):e15951. doi: 10.1371/journal.pone.0015951. PubMed PMID: 21283517; PubMed Central PMCID: PMC3023708.
    Staroń A, Finkeisen DE, Mascher T

    Peptide antibiotic sensing and detoxification modules of Bacillus subtilis

    Antimicrob Agents Chemother. 2011 Feb;55(2):515-25. doi: 10.1128/AAC.00352-10. PubMed PMID: 21078927; PubMed Central PMCID: PMC3028804.
    Ouyang J, Tian XL, Versey J, Wishart A, Li YH

    The BceABRS four-component system regulates the bacitracin-induced cell envelope stress response in Streptococcus mutans

    Antimicrob Agents Chemother. 2010 Sep;54(9):3895-906. doi: 10.1128/AAC.01802-09. PubMed PMID: 20606066; PubMed Central PMCID: PMC2935011.
    Rietkötter E, Hoyer D, Mascher T

    Bacitracin sensing in Bacillus subtilis

    Mol Microbiol. 2008 May;68(3):768-85. doi: 10.1111/j.1365-2958.2008.06194.x. PubMed PMID: 18394148.
    Bernard R, Guiseppi A, Chippaux M, Foglino M, Denizot F

    Resistance to bacitracin in Bacillus subtilis: unexpected requirement of the BceAB ABC transporter in the control of expression of its own structural genes

    J Bacteriol. 2007 Dec;189(23):8636-42. PubMed PMID: 17905982; PubMed Central PMCID: PMC2168949.
    Mascher T, Margulis NG, Wang T, Ye RW, Helmann JD

    Cell wall stress responses in Bacillus subtilis: the regulatory network of the bacitracin stimulon

    Mol Microbiol. 2003 Dec;50(5):1591-604. PubMed PMID: 14651641.
    Ohki R, Giyanto, Tateno K, Masuyama W, Moriya S, Kobayashi K, Ogasawara N

    The BceRS two-component regulatory system induces expression of the bacitracin transporter, BceAB, in Bacillus subtilis

    Mol Microbiol. 2003 Aug;49(4):1135-44. PubMed PMID: 12890034.
    Buckler DR, Zhou Y, Stock AM

    Evidence of intradomain and interdomain flexibility in an OmpR/PhoB homolog from Thermotoga maritima

    Structure. 2002 Feb;10(2):153-64. PubMed PMID: 11839301.
    Fabret C, Feher VA, Hoch JA

    Two-component signal transduction in Bacillus subtilis: how one organism sees its world

    J Bacteriol. 1999 Apr;181(7):1975-83. Review. PubMed PMID: 10094672; PubMed Central PMCID: PMC93607.
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