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Categories containing this gene/protein


transcription factors and their control, general stress proteins (controlled by SigB), resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons


BmrR regulon, Mta regulon, SigB regulon

The BmrR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,495,898 -> 2,496,734
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • MerR family
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 2BOW (complex with effector), 1R8E (complex with DNA), 1BOW
  • Localization

  • cell membrane (according to Swiss-Prot)
  • Interactions

    Additional information

    Expression and Regulation


    Operon

  • bmrU-bmr-bmrR PubMed
  • bmr-bmrR PubMed
  • Sigma factor

  • bmrU: SigB PubMed
  • bmr: SigA PubMed
  • Regulation

  • bmrU: induced by stress (SigB) PubMed
  • bmr: induced by rhodamine 6G and tetraphenylphosphonium (BmrR) PubMed
  • Regulatory mechanism

  • BmrR: transcription activation PubMed
  • Mta: transcription activation PubMed
  • Additional information

    Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Richard Brennan, Houston, Texas, USA Homepage
  • References


    Bachas S, Eginton C, Gunio D, Wade H

    Structural contributions to multidrug recognition in the multidrug resistance (MDR) gene regulator, BmrR

    Proc Natl Acad Sci U S A. 2011 Jul 5;108(27):11046-51. doi: 10.1073/pnas.1104850108. Epub 2011 Jun 20. PubMed PMID: 21690368; PubMed Central PMCID: PMC3131311.
    Kumaraswami M, Newberry KJ, Brennan RG

    Conformational plasticity of the coiled-coil domain of BmrR is required for bmr operator binding: the structure of unliganded BmrR

    J Mol Biol. 2010 Apr 30;398(2):264-75. doi: 10.1016/j.jmb.2010.03.011. Epub 2010 Mar 15. PubMed PMID: 20230832; PubMed Central PMCID: PMC2856848.
    Petersohn A, Brigulla M, Haas S, Hoheisel JD, Völker U, Hecker M

    Global analysis of the general stress response of Bacillus subtilis

    J Bacteriol. 2001 Oct;183(19):5617-31. PubMed PMID: 11544224; PubMed Central PMCID: PMC95453.
    Petersohn A, Antelmann H, Gerth U, Hecker M

    Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis

    Microbiology. 1999 Apr;145 ( Pt 4):869-80. PubMed PMID: 10220166.
    Baranova NN, Danchin A, Neyfakh AA

    Mta, a global MerR-type regulator of the Bacillus subtilis multidrug-efflux transporters

    Mol Microbiol. 1999 Mar;31(5):1549-59. PubMed PMID: 10200972.
    Ahmed M, Borsch CM, Taylor SS, Vázquez-Laslop N, Neyfakh AA

    A protein that activates expression of a multidrug efflux transporter upon binding the transporter substrates

    J Biol Chem. 1994 Nov 11;269(45):28506-13. PubMed PMID: 7961792.
    Ahmed M, Lyass L, Markham PN, Taylor SS, Vázquez-Laslop N, Neyfakh AA

    Two highly similar multidrug transporters of Bacillus subtilis whose expression is differentially regulated

    J Bacteriol. 1995 Jul;177(14):3904-10. PubMed PMID: 7608059; PubMed Central PMCID: PMC177116.
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