SubtiBank SubtiBank

Categories containing this gene/protein

acquisition of iron, iron metabolism, transcription factors and their control

This gene is a member of the following regulons

Fur regulon

The Btr regulon


  • Coordinates on the chromosome (coding sequence): 183,414 -> 185,003
  • The protein

    Catalyzed reaction/ biological activity

  • binding of a direct repeat in the feuA promoter region and transcription activation of the feuA-feuB-feuC-ybbA operon in the presence of bacillibactin or enterobactin PubMed
  • Protein family

  • AraC family
  • Paralogous protein(s)

    Kinetic information




  • enterobactin, bacillibactin (co-activators) PubMed
  • Effectors of protein activity

  • enterobactin, bacillibactin (co-activators) PubMed
  • Structure



  • Btr-RNA polymerase (perhaps SigA) PubMed
  • Additional information

    Expression and Regulation


  • btr PubMed
  • Sigma factor


  • expressed under conditions of iron starvation (Fur) PubMed
  • Regulatory mechanism

  • Fur: transcription repression PubMed
  • Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed
  • Biological materials


  • MGNA-B949 (ybbB::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein


    Leyn SA, Kazanov MD, Sernova NV, Ermakova EO, Novichkov PS, Rodionov DA

    Genomic reconstruction of the transcriptional regulatory network in Bacillus subtilis

    J Bacteriol. 2013 Jun;195(11):2463-73. doi: 10.1128/JB.00140-13. PubMed PMID: 23504016; PubMed Central PMCID: PMC3676070.
    Salzberg LI, Powell L, Hokamp K, Botella E, Noone D, Devine KM

    The WalRK (YycFG) and σ(I) RsgI regulators cooperate to control CwlO and LytE expression in exponentially growing and stressed Bacillus subtilis cells

    Mol Microbiol. 2013 Jan;87(1):180-95. doi: 10.1111/mmi.12092. PubMed PMID: 23199363.
    Gaballa A, MacLellan S, Helmann JD

    Transcription activation by the siderophore sensor Btr is mediated by ligand-dependent stimulation of promoter clearance

    Nucleic Acids Res. 2012 Apr;40(8):3585-95. doi: 10.1093/nar/gkr1280. PubMed PMID: 22210890; PubMed Central PMCID: PMC3333878.
    Gaballa A, Helmann JD

    Substrate induction of siderophore transport in Bacillus subtilis mediated by a novel one-component regulator

    Mol Microbiol. 2007 Oct;66(1):164-73. PubMed PMID: 17725565; PubMed Central PMCID: PMC3022416.
    Hambraeus G, von Wachenfeldt C, Hederstedt L

    Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs

    Mol Genet Genomics. 2003 Aug;269(5):706-14. PubMed PMID: 12884008.
    Baichoo N, Wang T, Ye R, Helmann JD

    Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon

    Mol Microbiol. 2002 Sep;45(6):1613-29. PubMed PMID: 12354229.
    No login