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Categories containing this gene/protein


carbon core metabolism, transcription factors and their control, regulators of core metabolism

This gene is a member of the following regulons


CcpA regulon, CcpC regulon

The CcpC regulon


Gene


  • Coordinates on the chromosome (coding sequence): 1,486,045 -> 1,486,926
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • LysR family
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

  • ykuJ-ykuK-abbA-ykuL-ccpC PubMed
  • ccpC PubMed
  • Sigma factor

  • ccpC: SigA
  • Regulation

  • repressed in the presence of glucose and glutamate (CcpC) PubMed
  • Regulatory mechanism

  • CcpC: transcription repression (molecular inducer: citrate) PubMed
  • CcpA: transcription repression PubMed
  • Additional information

    Biological materials


    Mutant

  • MGNA-A774 (ykuM::erm), available at the NBRP B. subtilis, Japan
  • GP206 (aphA3) & GP1442 (aphA3) , available in Stülke lab
  • Expression vector

  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP706, available in Stülke lab
  • lacZ fusion

  • pGP707 (in pAC5), available in Stülke lab, pGP711 (in pAC6), available in Stülke lab
  • GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
  • References


    Mittal M, Pechter KB, Picossi S, Kim HJ, Kerstein KO, Sonenshein AL

    Dual role of CcpC protein in regulation of aconitase gene expression in Listeria monocytogenes and Bacillus subtilis

    Microbiology. 2013 Jan;159(Pt 1):68-76. doi: 10.1099/mic.0.063388-0. Epub 2012 Nov 8. PubMed PMID: 23139400; PubMed Central PMCID: PMC3542727.
    Herzberg C, Weidinger LA, Dörrbecker B, Hübner S, Stülke J, Commichau FM

    SPINE: a method for the rapid detection and analysis of protein-protein interactions in vivo

    Proteomics. 2007 Nov;7(22):4032-5. PubMed PMID: 17994626.
    Blencke HM, Reif I, Commichau FM, Detsch C, Wacker I, Ludwig H, Stülke J

    Regulation of citB expression in Bacillus subtilis: integration of multiple metabolic signals in the citrate pool and by the general nitrogen regulatory system

    Arch Microbiol. 2006 Mar;185(2):136-46. Epub 2006 Jan 5. PubMed PMID: 16395550.
    Kim SI, Jourlin-Castelli C, Wellington SR, Sonenshein AL

    Mechanism of repression by Bacillus subtilis CcpC, a LysR family regulator

    J Mol Biol. 2003 Dec 5;334(4):609-24. PubMed PMID: 14636591.
    Kim HJ, Kim SI, Ratnayake-Lecamwasam M, Tachikawa K, Sonenshein AL, Strauch M

    Complex regulation of the Bacillus subtilis aconitase gene

    J Bacteriol. 2003 Mar;185(5):1672-80. PubMed PMID: 12591885; PubMed Central PMCID: PMC148081.
    Kim HJ, Jourlin-Castelli C, Kim SI, Sonenshein AL

    Regulation of the bacillus subtilis ccpC gene by ccpA and ccpC

    Mol Microbiol. 2002 Jan;43(2):399-410. PubMed PMID: 11985717.
    Jourlin-Castelli C, Mani N, Nakano MM, Sonenshein AL

    CcpC, a novel regulator of the LysR family required for glucose repression of the citB gene in Bacillus subtilis

    J Mol Biol. 2000 Jan 28;295(4):865-78. PubMed PMID: 10656796.
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