SubtiBank SubtiBank
'

Categories containing this gene/protein


carbon core metabolism, transcription factors and their control, regulators of core metabolism

This gene is a member of the following regulons


The CcpN regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,604,959 -> 2,605,597
  • Phenotypes of a mutant

  • Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed
  • Protein family

  • CcpN family
  • Paralogous protein(s)

    Kinetic information

    Domains

  • 2 CBS domains (83-139, 148-211)
  • Modification

    Cofactors

    Effectors of protein activity

  • YqfL (negative effector of CcpN activity) PubMed
  • ATP enhances CcpN-dependent repression, ADP counteracts the repressing activity of CcpN PubMed
  • Structure

    Localization

    Interactions

  • active as dimer (according to PubMed)
  • CcpN-RpoA PubMed
  • Additional information

    Expression and Regulation


    Operon

  • ccpN-yqfL PubMed
  • Sigma factor

    Regulation

  • constitutively expressed PubMed
  • Regulatory mechanism

    Additional information

  • the intracellular concentration of CcpN is about 4 myM (according to PubMed).
  • Biological materials


    Mutant

  • MGNA-C493 (yqzB::erm), available at the NBRP B. subtilis, Japan
  • DB104 ccpN::cat, available in Sabine Brantl's lab
  • GP1128 ccpN::cat, available in Jörg Stülke's lab
  • Expression vector

  • pGP2923 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
  • lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

  • available in Sabine Brantl's lab
  • Labs working on this gene/protein


  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
  • Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
  • Uwe Sauer, ETH Zürich, Switzerland homepage
  • References


    Reviews

    Brantl S(1), Licht A.

    Characterisation of Bacillus subtilis transcriptional regulators involved in metabolic processes.

    Curr Protein Pept Sci. 2010 Jun;11(4):274-91.

    Original Publications

    Declerck N, Royer CA

    Interactions in gene expression networks studied by two-photon fluorescence fluctuation spectroscopy

    Methods Enzymol. 2013;519:203-30. doi: 10.1016/B978-0-12-405539-1.00007-5. PubMed PMID: 23280112.
    Ferguson ML, Le Coq D, Jules M, Aymerich S, Radulescu O, Declerck N, Royer CA

    Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states

    Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):155-60. doi: 10.1073/pnas.1110541108. Epub 2011 Dec 21. PubMed PMID: 22190493; PubMed Central PMCID: PMC3252923.
    Eckart RA, Brantl S, Licht A

    Search for additional targets of the transcriptional regulator CcpN from Bacillus subtilis

    FEMS Microbiol Lett. 2009 Oct;299(2):223-31. doi: 10.1111/j.1574-6968.2009.01754.x. Epub 2009 Aug 6. PubMed PMID: 19732150.
    Licht A, Brantl S

    The transcriptional repressor CcpN from Bacillus subtilis uses different repression mechanisms at different promoters

    J Biol Chem. 2009 Oct 30;284(44):30032-8. doi: 10.1074/jbc.M109.033076. Epub 2009 Sep 2. PubMed PMID: 19726675; PubMed Central PMCID: PMC2781557.
    Tännler S, Fischer E, Le Coq D, Doan T, Jamet E, Sauer U, Aymerich S

    CcpN controls central carbon fluxes in Bacillus subtilis

    J Bacteriol. 2008 Sep;190(18):6178-87. doi: 10.1128/JB.00552-08. Epub 2008 Jun 27. PubMed PMID: 18586936; PubMed Central PMCID: PMC2546806.
    Licht A, Golbik R, Brantl S

    Identification of ligands affecting the activity of the transcriptional repressor CcpN from Bacillus subtilis

    J Mol Biol. 2008 Jun 27;380(1):17-30. doi: 10.1016/j.jmb.2008.05.002. Epub 2008 May 8. PubMed PMID: 18511073.
    Licht A, Brantl S

    Transcriptional repressor CcpN from Bacillus subtilis compensates asymmetric contact distribution by cooperative binding

    J Mol Biol. 2006 Dec 1;364(3):434-48. Epub 2006 Sep 12. PubMed PMID: 17011578.
    Licht A, Preis S, Brantl S

    Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in Bacillus subtilis

    Mol Microbiol. 2005 Oct;58(1):189-206. PubMed PMID: 16164558.
    Servant P, Le Coq D, Aymerich S

    CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes

    Mol Microbiol. 2005 Mar;55(5):1435-51. PubMed PMID: 15720552.
    '
    No login