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Categories containing this gene/protein


utilization of specific carbon sources, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons


The CitT regulon


Gene


  • Coordinates on the chromosome (coding sequence): 832,545 -> 833,225
  • Phenotypes of a mutant

  • no growth with citrate as sole carbon source PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • binding to the promoter of the citM-yflN operon and activation of transcription in the presence of citrate PubMed
  • Protein family

    Paralogous protein(s)

    Kinetic information

    Domains

    Modification

  • phosphorylated by CitS on an Asp residue
  • Cofactors

    Effectors of protein activity

  • phosphorylation likely affects DNA-binding activity
  • Structure

    Localization

  • cytoplasm (according to Swiss-Prot)
  • Interactions

  • CitS-CitT
  • Additional information

    Expression and Regulation


    Operon

  • citS-citT-yflP PubMed
  • Sigma factor

    Regulation

    Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • MGNA-C250 (citT::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Reviews

    Piepenbreier H(1), Fritz G(1), Gebhard S(2).

    Transporters as information processors in bacterial signalling pathways.

    Mol Microbiol. 2017 Apr;104(1):1-15. doi: 10.1111/mmi.13633. Epub 2017 Feb 24.DOI: 10.1111/mmi.13633 PMID: 28152228

    Original publications

    Repizo GD, Blancato VS, Sender PD, Lolkema J, Magni C

    Catabolite repression of the citST two-component system in Bacillus subtilis

    FEMS Microbiol Lett. 2006 Jul;260(2):224-31. PubMed PMID: 16842348.
    Yamamoto H, Murata M, Sekiguchi J

    The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis

    Mol Microbiol. 2000 Aug;37(4):898-912. PubMed PMID: 10972810.
    Fabret C, Feher VA, Hoch JA

    Two-component signal transduction in Bacillus subtilis: how one organism sees its world

    J Bacteriol. 1999 Apr;181(7):1975-83. Review. PubMed PMID: 10094672; PubMed Central PMCID: PMC93607.
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