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International Conference on Bacilli and Gram-positive bacteria
June 11 - 15, 2017, Berlin, Germany
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Categories containing this gene/protein
proteolysis, transcription factors and their control, general stress proteins (controlled by SigB), heat shock proteins, phosphoproteins
This gene is a member of the following regulons
CtsR regulon, SigB regulon
Coordinates on the chromosome (coding sequence): 101,449 -> 101,913
Catalyzed reaction/ biological activity
Protein familyctsR family (according to Swiss-Prot)
Modificationphosphorylated on Arg-55 (phosphorylation serves as tag for degradation by ClpC-ClpP) PubMedphosphorylation of a tyrosine residue by McsB PubMedrecently, it was reported that CtsR is phosphorylated by McsB on Arg-62 rather than on a tyrosine residue PubMedin addition, Arg-15 was reported to be a phosphorylation site PubMed
Effectors of protein activityCtsR is inactivated by heat, heat sensing requires Gly-64 PubMednon-phosphorylated McsB targets CtsR for degradation PubMedregulated proteolysis by ClpP/ClpC PubMed
Structure3H0D (complex with a 26bp DNA duplex, from Geobacillus stearothermophilus) PubMedMcsA-McsB-CtsRCtsR-ClpP/ClpC (degradation of CtsR) PubMed
Expression and Regulation
OperonctsR-mcsA-mcsB-clpC-radA-disA PubMedSigA PubMed, SigB PubMed2
Regulationinduced by stress (SigB) PubMedrepressed at low temperatures (CtsR) PubMed
Regulatory mechanismCtsR: transcription repression PubMed
Additional informationthe mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008" target="_blank">PubMed
MutantMGNA-B928 (yacG::erm), available at the NBRP B. subtilis, JapanctsR::aphA3 availbale in Ulf Gerth's labBKE00830 (ctsR::erm, available in the BGSC and in Jörg Stülke's lab)
Expression vectorfor expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
two-hybrid systemB. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
Antibodyavailable in Ulf Gerth's lab
Labs working on this gene/protein
Regulation of bacterial heat shock stimulons. Cell Stress Chaperones. 2016 Nov;21(6):959-968. Epub 2016 Aug 12.
Trentini DB, Suskiewicz MJ, Heuck A, Kurzbauer R, Deszcz L, Mechtler K,
Arginine phosphorylation marks proteins for degradation by a Clp
protease Nature. 2016 Nov 3;539(7627):48-53. doi: 10.1038/nature20122. Epub 2016
Oct 6. PubMed PMID: 27749819.
Battesti A, Gottesman S
Roles of adaptor proteins in regulation of bacterial
proteolysis Curr Opin Microbiol. 2013 Apr;16(2):140-7. doi:
10.1016/j.mib.2013.01.002. Epub 2013 Jan 31. Review. PubMed PMID: 23375660;
PubMed Central PMCID: PMC3646950.
Elsholz AK, Gerth U, Hecker M
Regulation of CtsR activity in low GC, Gram+
bacteria Adv Microb Physiol. 2010;57:119-44. doi:
10.1016/B978-0-12-381045-8.00003-5. Review. PubMed PMID: 21078442.
The Bacillus subtilis heat shock stimulon Cell Stress Chaperones.
2003 Fall;8(3):207-17. Review. PubMed PMID: 14984053; PubMed Central PMCID: