SubtiBank SubtiBank
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Categories containing this gene/protein


utilization of lipids, transcription factors and their control

This gene is a member of the following regulons


CcpA regulon, FadR regulon

The FadR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,917,957 -> 2,918,541
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • TetR family
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

  • long chain fatty acids act as inducers that release FadR from its operator sites PubMed
  • Structure

    Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

  • lcfA-fadR-fadB-etfB-etfA PubMed
  • fadR-fadB-etfB-etfA PubMed
  • Sigma factor

  • lcfA: SigA PubMed
  • fadR: SigA PubMed
  • Regulation

  • induced by long-chain fatty acids (FadR) PubMed
  • subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed
  • Regulatory mechanism

  • FadR: transcription repression PubMed
  • CcpA-HPr(Ser-P): transcription repression PubMed
  • Additional information

    Biological materials


    Mutant

  • MGNA-B008 (ysiA::erm), available at the NBRP B. subtilis, Japan
  • 1A853 ( fadR::cat), PubMed, available at BGSC
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Reviews

    Cuthbertson L, Nodwell JR

    The TetR family of regulators

    Microbiol Mol Biol Rev. 2013 Sep;77(3):440-75. doi: 10.1128/MMBR.00018-13. Review. PubMed PMID: 24006471; PubMed Central PMCID: PMC3811609.
    Fujita Y, Matsuoka H, Hirooka K

    Regulation of fatty acid metabolism in bacteria

    Mol Microbiol. 2007 Nov;66(4):829-39. Review. PubMed PMID: 17919287.

    Original publications

    Fujihashi M, Nakatani T, Hirooka K, Matsuoka H, Fujita Y, Miki K

    Structural characterization of a ligand-bound form of Bacillus subtilis FadR involved in the regulation of fatty acid degradation

    Proteins. 2014 Jul;82(7):1301-10. doi: 10.1002/prot.24496. PubMed PMID: 24356978.
    Tojo S, Satomura T, Matsuoka H, Hirooka K, Fujita Y

    Catabolite repression of the Bacillus subtilis FadR regulon, which is involved in fatty acid catabolism

    J Bacteriol. 2011 May;193(10):2388-95. doi: 10.1128/JB.00016-11. PubMed PMID: 21398533; PubMed Central PMCID: PMC3133144.
    Matsuoka H, Hirooka K, Fujita Y

    Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation

    J Biol Chem. 2007 Feb 23;282(8):5180-94. PubMed PMID: 17189250.
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