SubtiBank SubtiBank
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Categories containing this gene/protein


electron transport/ other, lipid metabolism/ other, transcription factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), phosphoproteins

This gene is a member of the following regulons


FatR regulon, SigM regulon, SigW regulon, SigX regulon

The FatR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,777,070 -> 2,777,654
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

    Paralogous protein(s)

    Kinetic information

    Domains

    Modification

  • phosphorylated by PtkA on Tyr-45, this results in displacement of FatR from the DNA PubMed
  • Cofactors

    Effectors of protein activity

    Structure

    Localization

    Interactions

  • FatR-TkmA PubMed
  • FatR-PtkA PubMed
  • Additional information

    Expression and Regulation


    Operon

  • fatR-yrhJ PubMed
  • yrhH-fatR-yrhJ PubMed
  • Sigma factor

  • SigM PubMed, SigW PubMed, SigX PubMed
  • Regulation

  • induced by unsaturated fatty acids (FatR) PubMed
  • expression is reduced in a SigV mutant PubMed
  • Regulatory mechanism

  • FatR: transcription repression PubMed
  • Additional information

    Biological materials


    Mutant

  • MGNA-A177 (yrhI::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Derouiche A, Bidnenko V, Grenha R, Pigonneau N, Ventroux M, Franz-Wachtel M, Nessler S, Noirot-Gros MF, Mijakovic I

    Interaction of bacterial fatty-acid-displaced regulators with DNA is interrupted by tyrosine phosphorylation in the helix-turn-helix domain

    Nucleic Acids Res. 2013 Nov;41(20):9371-81. doi: 10.1093/nar/gkt709. PubMed PMID: 23939619; PubMed Central PMCID: PMC3814354.
    Guariglia-Oropeza V, Helmann JD

    Bacillus subtilis σ(V) confers lysozyme resistance by activation of two cell wall modification pathways, peptidoglycan O-acetylation and D-alanylation of teichoic acids

    J Bacteriol. 2011 Nov;193(22):6223-32. doi: 10.1128/JB.06023-11. PubMed PMID: 21926231; PubMed Central PMCID: PMC3209214.
    Cao M, Wang T, Ye R, Helmann JD

    Antibiotics that inhibit cell wall biosynthesis induce expression of the Bacillus subtilis sigma(W) and sigma(M) regulons

    Mol Microbiol. 2002 Sep;45(5):1267-76. PubMed PMID: 12207695.
    Gustafsson MC, Palmer CN, Wolf CR, von Wachenfeldt C

    Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species

    Arch Microbiol. 2001 Dec;176(6):459-64. PubMed PMID: 11734890.
    Lee TR, Hsu HP, Shaw GC

    Transcriptional regulation of the Bacillus subtilis bscR-CYP102A3 operon by the BscR repressor and differential induction of cytochrome CYP102A3 expression by oleic acid and palmitate

    J Biochem. 2001 Oct;130(4):569-74. PubMed PMID: 11574077.
    Gustafsson MC, von Wachenfeldt C

    A novel diffusible substance can overcome the apparent AbrB repression of the Bacillus subtilis fatR promoter

    FEMS Microbiol Lett. 2001 May 30;199(2):197-202. PubMed PMID: 11377867.
    Palmer CN, Gustafsson MC, Dobson H, von Wachenfeldt C, Wolf CR

    Adaptive responses to fatty acids are mediated by the regulated expression of cytochromes P450

    Biochem Soc Trans. 1999 Aug;27(4):374-8. Review. PubMed PMID: 10917605.
    Huang X, Helmann JD

    Identification of target promoters for the Bacillus subtilis sigma X factor using a consensus-directed search

    J Mol Biol. 1998 May 29;279(1):165-73. PubMed PMID: 9636707.
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