SubtiBank SubtiBank

Categories containing this gene/protein

DNA condensation/ segregation, essential genes, membrane proteins, phosphoproteins

This gene is a member of the following regulons

LexA regulon, SigH regulon


  • Coordinates on the chromosome (coding sequence): 2,385,543 -> 2,385,821
  • Phenotypes of a mutant

  • essential PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • Hbs-mediated untwisting of the DNA stimulates Nth endonuclease activity at AP sites PubMed
  • Protein family

  • DbpA subfamily (according to Swiss-Prot)
  • Paralogous protein(s)

  • YonN
  • Kinetic information



  • phosphorylated on Arg-61 PubMed
  • phosphorylation on Thr-4 PubMed
  • Cofactors

    Effectors of protein activity


  • 1HUU (Geobacillus stearothermophilus)
  • Localization

  • associated with the nucleoid in vegetative cells and forespores PubMed
  • membrane associated PubMed
  • Interactions

    Additional information

    Expression and Regulation


  • hbs PubMed
  • Sigma factor

  • SigA PubMed, SigH PubMed
  • Regulation

  • induced by DNA damage (LexA) PubMed
  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • Regulatory mechanism

  • LexA: transcription repression PubMed
  • Additional information

  • mRNA is protected from degradation by RnjA by initiating ribosomes PubMed
  • Hbs is present with about 50,000 copies per genome in vegetative cells and spores, it is one of the most abundant proteins in B. subtilis PubMed
  • Biological materials


    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
  • FLAG-tag construct


    Labs working on this gene/protein

  • Ciaran Condon, IBPC, Paris, France Homepage
  • Mohamed Marahiel, University of Marburg, Germany Homepage
  • References


    Klein W(1), Marahiel MA.

    Structure-function relationship and regulation of two Bacillus subtilis DNA-binding proteins, HBsu and AbrB.

    J Mol Microbiol Biotechnol. 2002 May;4(3):323-9.

    Original publications

    de Jong IG, Veening JW, Kuipers OP

    Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation

    Environ Microbiol. 2012 Dec;14(12):3110-21. doi: 10.1111/j.1462-2920.2012.02892.x. Epub 2012 Oct 4. PubMed PMID: 23033921.
    Elsholz AK, Turgay K, Michalik S, Hessling B, Gronau K, Oertel D, Mäder U, Bernhardt J, Becher D, Hecker M, Gerth U

    Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis

    Proc Natl Acad Sci U S A. 2012 May 8;109(19):7451-6. doi: 10.1073/pnas.1117483109. Epub 2012 Apr 19. PubMed PMID: 22517742; PubMed Central PMCID: PMC3358850.
    Collier C, Machón C, Briggs GS, Smits WK, Soultanas P

    Untwisting of the DNA helix stimulates the endonuclease activity of Bacillus subtilis Nth at AP sites

    Nucleic Acids Res. 2012 Jan;40(2):739-50. doi: 10.1093/nar/gkr785. Epub 2011 Sep 27. PubMed PMID: 21954439; PubMed Central PMCID: PMC3258159.
    Daou-Chabo R, Condon C

    RNase J1 endonuclease activity as a probe of RNA secondary structure

    RNA. 2009 Jul;15(7):1417-25. doi: 10.1261/rna.1574309. Epub 2009 May 20. PubMed PMID: 19458035; PubMed Central PMCID: PMC2704070.
    Daou-Chabo R, Mathy N, Bénard L, Condon C

    Ribosomes initiating translation of the hbs mRNA protect it from 5'-to-3' exoribonucleolytic degradation by RNase J1

    Mol Microbiol. 2009 Mar;71(6):1538-50. doi: 10.1111/j.1365-2958.2009.06620.x. Epub 2009 Feb 2. PubMed PMID: 19210617.
    Hahne H, Wolff S, Hecker M, Becher D

    From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches

    Proteomics. 2008 Oct;8(19):4123-36. doi: 10.1002/pmic.200800258. PubMed PMID: 18763711.
    Macek B, Mijakovic I, Olsen JV, Gnad F, Kumar C, Jensen PR, Mann M

    The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis

    Mol Cell Proteomics. 2007 Apr;6(4):697-707. Epub 2007 Jan 10. PubMed PMID: 17218307.
    Au N, Kuester-Schoeck E, Mandava V, Bothwell LE, Canny SP, Chachu K, Colavito SA, Fuller SN, Groban ES, Hensley LA, O'Brien TC, Shah A, Tierney JT, Tomm LL, O'Gara TM, Goranov AI, Grossman AD, Lovett CM

    Genetic composition of the Bacillus subtilis SOS system

    J Bacteriol. 2005 Nov;187(22):7655-66. PubMed PMID: 16267290; PubMed Central PMCID: PMC1280312.
    Ross MA, Setlow P

    The Bacillus subtilis HBsu protein modifies the effects of alpha/beta-type, small acid-soluble spore proteins on DNA

    J Bacteriol. 2000 Apr;182(7):1942-8. PubMed PMID: 10715001; PubMed Central PMCID: PMC101886.
    Fernández S, Alonso JC

    Bacillus subtilis sequence-independent DNA-binding and DNA-bending protein Hbsu negatively controls its own synthesis

    Gene. 1999 Apr 29;231(1-2):187-93. PubMed PMID: 10231583.
    Köhler P, Marahiel MA

    Mutational analysis of the nucleoid-associated protein HBsu of Bacillus subtilis

    Mol Gen Genet. 1998 Dec;260(5):487-91. PubMed PMID: 9894920.
    Fernández S, Rojo F, Alonso JC

    The Bacillus subtilis chromatin-associated protein Hbsu is involved in DNA repair and recombination

    Mol Microbiol. 1997 Mar;23(6):1169-79. PubMed PMID: 9106208.
    Köhler P, Marahiel MA

    Association of the histone-like protein HBsu with the nucleoid of Bacillus subtilis

    J Bacteriol. 1997 Mar;179(6):2060-4. PubMed PMID: 9068655; PubMed Central PMCID: PMC178933.
    Alonso JC, Gutierrez C, Rojo F

    The role of chromatin-associated protein Hbsu in beta-mediated DNA recombination is to facilitate the joining of distant recombination sites

    Mol Microbiol. 1995 Nov;18(3):471-8. PubMed PMID: 8748031.
    Alonso JC, Weise F, Rojo F

    The Bacillus subtilis histone-like protein Hbsu is required for DNA resolution and DNA inversion mediated by the beta recombinase of plasmid pSM19035

    J Biol Chem. 1995 Feb 17;270(7):2938-45. PubMed PMID: 7852372.
    Micka B, Groch N, Heinemann U, Marahiel MA

    Molecular cloning, nucleotide sequence, and characterization of the Bacillus subtilis gene encoding the DNA-binding protein HBsu

    J Bacteriol. 1991 May;173(10):3191-8. PubMed PMID: 1902464; PubMed Central PMCID: PMC207914.
    Micka B, Marahiel MA

    The DNA-binding protein HBsu is essential for normal growth and development in Bacillus subtilis

    Biochimie. 1992 Jul-Aug;74(7-8):641-50. PubMed PMID: 1382620.
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