SubtiBank SubtiBank

Categories containing this gene/protein


biosynthesis/ acquisition of nucleotides, transcription factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons


SigM regulon

The HprT regulon


Gene


  • Coordinates on the chromosome (coding sequence): 76,344 -> 76,886
  • Phenotypes of a mutant

  • essential PubMed, non-essential according to PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • IMP diphosphate = hypoxanthine 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)
  • Protein family

  • purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

  • inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´PubMed
  • Structure

  • 1J7J (from Salmonella typhimurium, 51% identity, 73% similarity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Interactions

  • TilS-HprT, the complex activates transcription of ftsH PubMed
  • Additional information

    Expression and Regulation


    Operon

  • tilS-hprT-ftsH PubMed
  • Sigma factor

  • SigM PubMed
  • Regulation

    Regulatory mechanism

    Additional information

    Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Reviews

    Hove-Jensen B(1,)(2), Andersen KR(2), Kilstrup M(3), Martinussen J(3), Switzer RL(4), Willemoës M(5).

    Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.

    Microbiol Mol Biol Rev. 2016 Dec 28;81(1). pii: e00040-16. doi: 10.1128/MMBR.00040-16. Print 2017 Mar.DOI: 10.1128/MMBR.00040-16 PMCID: PMC5312242 [Available on 2017-12-28] PMID: 28031352

    Original publications

    Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA

    Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis

    Cell Syst. 2017 Mar 22;4(3):291-305.e7. doi: 10.1016/j.cels.2016.12.013. Epub 2017 Feb 8. PubMed PMID: 28189581.
    Lin TH, Hu YN, Shaw GC

    Two enzymes, TilS and HprT, can form a complex to function as a transcriptional activator for the cell division protease gene ftsH in Bacillus subtilis

    J Biochem. 2014 Jan;155(1):5-16. doi: 10.1093/jb/mvt081. PubMed PMID: 24001521.
    Kriel A, Bittner AN, Kim SH, Liu K, Tehranchi AK, Zou WY, Rendon S, Chen R, Tu BP, Wang JD

    Direct regulation of GTP homeostasis by (p)ppGpp: a critical component of viability and stress resistance

    Mol Cell. 2012 Oct 26;48(2):231-41. doi: 10.1016/j.molcel.2012.08.009. PubMed PMID: 22981860; PubMed Central PMCID: PMC3483369.
    Eiamphungporn W, Helmann JD

    The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses

    Mol Microbiol. 2008 Feb;67(4):830-48. doi: 10.1111/j.1365-2958.2007.06090.x. PubMed PMID: 18179421; PubMed Central PMCID: PMC3025603.
    Endo T, Uratani B, Freese E

    Purine salvage pathways of Bacillus subtilis and effect of guanine on growth of GMP reductase mutants

    J Bacteriol. 1983 Jul;155(1):169-79. PubMed PMID: 6408059; PubMed Central PMCID: PMC217666.
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