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Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, transcription factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigM regulon

The HprT regulon


  • Coordinates on the chromosome (coding sequence): 76,344 -> 76,886
  • Phenotypes of a mutant

  • essential PubMed, non-essential according to PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • IMP diphosphate = hypoxanthine 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)
  • Protein family

  • purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information




    Effectors of protein activity

  • inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´PubMed
  • Structure

  • 1J7J (from Salmonella typhimurium, 51% identity, 73% similarity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Interactions

  • TilS-HprT, the complex activates transcription of ftsH PubMed
  • Additional information

    Expression and Regulation


  • tilS-hprT-ftsH PubMed
  • Sigma factor

  • SigM PubMed
  • Regulation

    Regulatory mechanism

    Additional information

    Biological materials


    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein



    Hove-Jensen B(1,)(2), Andersen KR(2), Kilstrup M(3), Martinussen J(3), Switzer RL(4), Willemoës M(5).

    Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.

    Microbiol Mol Biol Rev. 2016 Dec 28;81(1). pii: e00040-16. doi: 10.1128/MMBR.00040-16. Print 2017 Mar.DOI: 10.1128/MMBR.00040-16 PMCID: PMC5312242 [Available on 2017-12-28] PMID: 28031352

    Original publications

    Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA

    Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis

    Cell Syst. 2017 Mar 22;4(3):291-305.e7. doi: 10.1016/j.cels.2016.12.013. Epub 2017 Feb 8. PubMed PMID: 28189581; PubMed Central PMCID: PMC5400513.
    Lin TH, Hu YN, Shaw GC

    Two enzymes, TilS and HprT, can form a complex to function as a transcriptional activator for the cell division protease gene ftsH in Bacillus subtilis

    J Biochem. 2014 Jan;155(1):5-16. doi: 10.1093/jb/mvt081. Epub 2013 Sep 3. PubMed PMID: 24001521.
    Kriel A, Bittner AN, Kim SH, Liu K, Tehranchi AK, Zou WY, Rendon S, Chen R, Tu BP, Wang JD

    Direct regulation of GTP homeostasis by (p)ppGpp: a critical component of viability and stress resistance

    Mol Cell. 2012 Oct 26;48(2):231-41. doi: 10.1016/j.molcel.2012.08.009. Epub 2012 Sep 13. PubMed PMID: 22981860; PubMed Central PMCID: PMC3483369.
    Eiamphungporn W, Helmann JD

    The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses

    Mol Microbiol. 2008 Feb;67(4):830-48. doi: 10.1111/j.1365-2958.2007.06090.x. Epub 2008 Jan 2. PubMed PMID: 18179421; PubMed Central PMCID: PMC3025603.
    Endo T, Uratani B, Freese E

    Purine salvage pathways of Bacillus subtilis and effect of guanine on growth of GMP reductase mutants

    J Bacteriol. 1983 Jul;155(1):169-79. PubMed PMID: 6408059; PubMed Central PMCID: PMC217666.
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