SubtiBank SubtiBank

Categories containing this gene/protein


transcription factors and their control, heat shock proteins

This gene is a member of the following regulons


HrcA regulon

The HrcA regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,628,606 -> 2,629,637
  • The protein


    Catalyzed reaction/ biological activity

  • transcription repressor of the hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV and groES-groEL operons (at low/ normal temperatures)
  • Protein family

  • hrcA family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

  • phosphorylated on arginine residues PubMed
  • Cofactors

  • GroEL acts as co-repressor
  • Effectors of protein activity

    Structure

    Localization

  • cytoplasm (according to Swiss-Prot)
  • Interactions

  • GroEL-HrcA
  • Additional information

    Expression and Regulation


    Operon

  • hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-mtaB PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • induced by heat shock (HrcA) PubMed
  • Regulatory mechanism

  • HrcA: transcription repression PubMed
  • Additional information

    Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Wolfgang Schumann, Bayreuth University, Germany Homepage
  • Thomas Wiegert, University of Bayreuth, Germany Homepage
  • References


    Reviews

    Schumann W(1).

    Regulation of bacterial heat shock stimulons.

    Cell Stress Chaperones. 2016 Nov;21(6):959-968. Epub 2016 Aug 12.

    Original Publications

    Schmidt A, Trentini DB, Spiess S, Fuhrmann J, Ammerer G, Mechtler K, Clausen T

    Quantitative phosphoproteomics reveals the role of protein arginine phosphorylation in the bacterial stress response

    Mol Cell Proteomics. 2014 Feb;13(2):537-50. doi: 10.1074/mcp.M113.032292. PubMed PMID: 24263382; PubMed Central PMCID: PMC3916652.
    Wiegert T, Schumann W

    Analysis of a DNA-binding motif of the Bacillus subtilis HrcA repressor protein

    FEMS Microbiol Lett. 2003 Jun 6;223(1):101-6. PubMed PMID: 12799007.
    Reischl S, Wiegert T, Schumann W

    Isolation and analysis of mutant alleles of the Bacillus subtilis HrcA repressor with reduced dependency on GroE function

    J Biol Chem. 2002 Sep 6;277(36):32659-67. PubMed PMID: 12082092.
    Helmann JD, Wu MF, Kobel PA, Gamo FJ, Wilson M, Morshedi MM, Navre M, Paddon C

    Global transcriptional response of Bacillus subtilis to heat shock

    J Bacteriol. 2001 Dec;183(24):7318-28. PubMed PMID: 11717291; PubMed Central PMCID: PMC95581.
    Homuth G, Mogk A, Schumann W

    Post-transcriptional regulation of the Bacillus subtilis dnaK operon

    Mol Microbiol. 1999 Jun;32(6):1183-97. PubMed PMID: 10383760.
    Mogk A, Homuth G, Scholz C, Kim L, Schmid FX, Schumann W

    The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis

    EMBO J. 1997 Aug 1;16(15):4579-90. PubMed PMID: 9303302; PubMed Central PMCID: PMC1170084.
    Mogk A, Homuth G, Scholz C, Kim L, Schmid FX, Schumann W

    The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis

    EMBO J. 1997 Aug 1;16(15):4579-90. PubMed PMID: 9303302; PubMed Central PMCID: PMC1170084.
    Homuth G, Masuda S, Mogk A, Kobayashi Y, Schumann W

    The dnaK operon of Bacillus subtilis is heptacistronic

    J Bacteriol. 1997 Feb;179(4):1153-64. PubMed PMID: 9023197; PubMed Central PMCID: PMC178811.
    Schulz A, Schumann W

    hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat shock genes

    J Bacteriol. 1996 Feb;178(4):1088-93. PubMed PMID: 8576042; PubMed Central PMCID: PMC177769.
    Zuber U, Schumann W

    CIRCE, a novel heat shock element involved in regulation of heat shock operon dnaK of Bacillus subtilis

    J Bacteriol. 1994 Mar;176(5):1359-63. PubMed PMID: 8113175; PubMed Central PMCID: PMC205200.
    Yuan G, Wong SL

    Isolation and characterization of Bacillus subtilis groE regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK

    J Bacteriol. 1995 Nov;177(22):6462-8. PubMed PMID: 7592421; PubMed Central PMCID: PMC177496.
    Schulz A, Tzschaschel B, Schumann W

    Isolation and analysis of mutants of the dnaK operon of Bacillus subtilis

    Mol Microbiol. 1995 Feb;15(3):421-9. PubMed PMID: 7540247.
    Wetzstein M, Völker U, Dedio J, Löbau S, Zuber U, Schiesswohl M, Herget C, Hecker M, Schumann W

    Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis

    J Bacteriol. 1992 May;174(10):3300-10. PubMed PMID: 1339421; PubMed Central PMCID: PMC205999.
    Wetzstein M, Völker U, Dedio J, Löbau S, Zuber U, Schiesswohl M, Herget C, Hecker M, Schumann W

    Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis

    J Bacteriol. 1992 May;174(10):3300-10. PubMed PMID: 1339421; PubMed Central PMCID: PMC205999.
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