SubtiBank SubtiBank

Description: MarR/DUF24 family transcription regulator, positively controls the nitroreductase gene hypO in response to disulfide stress

Name: hypR
Locus: BSU40540BsubCyc
pI: 8.4
MW: 14.0 kDa
Protein length: 125 aaBLASTSequence
Gene length: 375 bpBLASTSequenceSequence + Flanks
Function: control of the nitroreductase gene hypO in response to disulfide stress (diamide, NaOCl)
Product: MarR/DUF24 family transcription regulator HypR
Essential: no
E.C. number:
Synonyms: yybR
Databases: SubtiListKEGGUniProtExpression data browser
'

Categories containing this gene/protein


transcription factors and their control, resistance against oxidative and electrophile stress

This gene is a member of the following regulons


HypR regulon

The HypR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 4,167,622 -> 4,167,999
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • [[MarR/DUF24 family]]
  • Paralogous protein(s)

  • YdeP,YkvN
  • Kinetic information

  • Cys14 redox sensing Cys, has lower pKa of 6.36 PubMed
  • Domains

  • 5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface PubMed
  • Modification

  • oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress PubMed
  • Cys14 and Cys49' are about 8-9 Angström apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha4 helices of the HypR dimer about 4 Angstroem towards each other that leads to activation of HypR PubMed
  • Cofactors

    Effectors of protein activity

    Structure

  • 4A5N (reduced HypRC14S dimer) PubMed
  • 4A5M (oxidized HypR C14-C49' intersubunit disulfide-linked dimer ) PubMed
  • Localization

  • cytoplasmic
  • Interactions

    Additional information

    Expression and Regulation


    Operon

  • hypR PubMed
  • Sigma factor

    Regulation

  • activated by disulfide stress conditions (diamide, NaOCl) in vivo and in vitro (autoregulation) PubMed
  • Regulatory mechanism

  • HypR: transcription activation (autoregulation)PubMed
  • Additional information

    Biological materials


    Mutant

  • MGNA-B836 (yybR::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Haike Antelmann,University of Greifswald, Germany
  • References


    '
    No login