SubtiBank SubtiBank

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

RplT regulon, stringent response


  • Coordinates on the chromosome (coding sequence): 2,952,828 -> 2,953,349
  • Phenotypes of a mutant

  • essential PubMed
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

  • IF-3 family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information




    Effectors of protein activity


  • 1TIF (N-terminal domain, Geobacillus stearothermophilus), 1TIG (C-terminal domain, Geobacillus stearothermophilus)
  • Localization


    Additional information

    Expression and Regulation


  • infC-rpmI-rplT-ysdA PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism

  • binding of RplT to a RNA switch in the infC leader region causes transcription termination PubMed
  • Additional information

  • expression of IF3 is uncoupled from that of L35 and L20 by RNase Y-dependent processing PubMed
  • Biological materials


    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein


    Elvekrog MM, Gonzalez RL Jr

    Conformational selection of translation initiation factor 3 signals proper substrate selection

    Nat Struct Mol Biol. 2013 May;20(5):628-33. doi: 10.1038/nsmb.2554. Epub 2013 Apr 14. PubMed PMID: 23584454; PubMed Central PMCID: PMC3648635.
    Bruscella P, Shahbabian K, Laalami S, Putzer H

    RNase Y is responsible for uncoupling the expression of translation factor IF3 from that of the ribosomal proteins L35 and L20 in Bacillus subtilis

    Mol Microbiol. 2011 Sep;81(6):1526-41. doi: 10.1111/j.1365-2958.2011.07793.x. Epub 2011 Aug 15. PubMed PMID: 21843271.
    Sojka L, Kouba T, Barvík I, Sanderová H, Maderová Z, Jonák J, Krásny L

    Rapid changes in gene expression: DNA determinants of promoter regulation by the concentration of the transcription initiating NTP in Bacillus subtilis

    Nucleic Acids Res. 2011 Jun;39(11):4598-611. doi: 10.1093/nar/gkr032. Epub 2011 Feb 7. PubMed PMID: 21303765; PubMed Central PMCID: PMC3113569.
    Choonee N, Even S, Zig L, Putzer H

    Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism

    Nucleic Acids Res. 2007;35(5):1578-88. Epub 2007 Feb 8. PubMed PMID: 17289755; PubMed Central PMCID: PMC1865079.
    Eymann C, Homuth G, Scharf C, Hecker M

    Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis

    J Bacteriol. 2002 May;184(9):2500-20. PubMed PMID: 11948165; PubMed Central PMCID: PMC134987.
    Biou V, Shu F, Ramakrishnan V

    X-ray crystallography shows that translational initiation factor IF3 consists of two compact alpha/beta domains linked by an alpha-helix

    EMBO J. 1995 Aug 15;14(16):4056-64. PubMed PMID: 7664745; PubMed Central PMCID: PMC394484.
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