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Categories containing this gene/protein


DNA repair/ recombination, transcription factors and their control

This gene is a member of the following regulons


LexA regulon

The LexA regulon


Gene


  • Coordinates on the chromosome (coding sequence): 1,917,639 -> 1,918,256
  • The protein


    Catalyzed reaction/ biological activity

  • Represses uvrB, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5-CGAACNNNNGTTCG-3. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)
  • Protein family

  • peptidase S24 family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 1JHH (from E. coli, S119A mutant, 33% identity, 52% similarity) PubMed
  • Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

  • lexA PubMed
  • Sigma factor

    Regulation

  • induced upon DNA damage (LexA) PubMed
  • Regulatory mechanism

  • autorepression
  • LexA: transcription repression PubMed
  • Additional information

  • A ncRNA is predicted between fosB and lexA PubMed
  • Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Reviews

    Lenhart JS, Schroeder JW, Walsh BW, Simmons LA

    DNA repair and genome maintenance in Bacillus subtilis

    Microbiol Mol Biol Rev. 2012 Sep;76(3):530-64. doi: 10.1128/MMBR.05020-11. Review. PubMed PMID: 22933559; PubMed Central PMCID: PMC3429619.
    Butala M, Zgur-Bertok D, Busby SJ

    The bacterial LexA transcriptional repressor

    Cell Mol Life Sci. 2009 Jan;66(1):82-93. doi: 10.1007/s00018-008-8378-6. Review. PubMed PMID: 18726173.

    Original publications

    Irnov I, Sharma CM, Vogel J, Winkler WC

    Identification of regulatory RNAs in Bacillus subtilis

    Nucleic Acids Res. 2010 Oct;38(19):6637-51. doi: 10.1093/nar/gkq454. Epub 2010 Jun 4. PubMed PMID: 20525796; PubMed Central PMCID: PMC2965217.
    Kawai Y, Ogasawara N

    Bacillus subtilis EzrA and FtsL synergistically regulate FtsZ ring dynamics during cell division

    Microbiology. 2006 Apr;152(Pt 4):1129-41. PubMed PMID: 16549676.
    Au N, Kuester-Schoeck E, Mandava V, Bothwell LE, Canny SP, Chachu K, Colavito SA, Fuller SN, Groban ES, Hensley LA, O'Brien TC, Shah A, Tierney JT, Tomm LL, O'Gara TM, Goranov AI, Grossman AD, Lovett CM

    Genetic composition of the Bacillus subtilis SOS system

    J Bacteriol. 2005 Nov;187(22):7655-66. PubMed PMID: 16267290; PubMed Central PMCID: PMC1280312.
    Luo Y, Pfuetzner RA, Mosimann S, Paetzel M, Frey EA, Cherney M, Kim B, Little JW, Strynadka NC

    Crystal structure of LexA: a conformational switch for regulation of self-cleavage

    Cell. 2001 Sep 7;106(5):585-94. PubMed PMID: 11551506.
    Winterling KW, Chafin D, Hayes JJ, Sun J, Levine AS, Yasbin RE, Woodgate R

    The Bacillus subtilis DinR binding site: redefinition of the consensus sequence

    J Bacteriol. 1998 Apr;180(8):2201-11. PubMed PMID: 9555905; PubMed Central PMCID: PMC107149.
    Winterling KW, Levine AS, Yasbin RE, Woodgate R

    Characterization of DinR, the Bacillus subtilis SOS repressor

    J Bacteriol. 1997 Mar;179(5):1698-703. PubMed PMID: 9045831; PubMed Central PMCID: PMC178884.
    Miller MC, Resnick JB, Smith BT, Lovett CM Jr

    The bacillus subtilis dinR gene codes for the analogue of Escherichia coli LexA

    Purification and characterization of the DinR protein. J Biol Chem. 1996 Dec 27;271(52):33502-8. PubMed PMID: 8969214.
    Haijema BJ, van Sinderen D, Winterling K, Kooistra J, Venema G, Hamoen LW

    Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis

    Mol Microbiol. 1996 Oct;22(1):75-85. PubMed PMID: 8899710.
    Raymond-Denise A, Guillen N

    Identification of dinR, a DNA damage-inducible regulator gene of Bacillus subtilis

    J Bacteriol. 1991 Nov;173(22):7084-91. PubMed PMID: 1657879; PubMed Central PMCID: PMC209213.
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