lexA
168
transcriptional repressor of the SOS regulon
locus
BSU_17850
Molecular weight
22.70 kDa
pI
5.16
function
regulation of DNA damage repair
product
transcriptional repressor of the SOS regulon
essential
no
synonyms
lexA, dinR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1974 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,917,639 → 1,918,256
Advertisement
The protein
Catalyzed reaction/ biological activity
Represses ''[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB], [gene|DEB7605B4F6F5F365A49C1C15647F9767B01BF66|bstG], [gene|60344F56DEB167FA62C16E52E8238393CEE19312|tagC], [gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]'' genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] interacts with [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA] causing an autocatalytic cleavage which disrupts the DNA-binding part of [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA], leading to derepression of the SOS regulon and eventually DNA repair (according to UniProt)
Hydrolysis of Ala-|-Gly bond in repressor LexA (according to UniProt)
Protein family
peptidase S24 family (single member, according to UniProt)
Structure
[PDB|1JHH] (from ''E. coli'', S119A mutant, 33% identity, 52% similarity) [Pubmed|11551506]
[AF|P31080]
Expression and Regulation
Operons
genes
[gene|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]
description
[Pubmed|1657879]
regulation
induced upon DNA damage ([protein|search|LexA]) [Pubmed|1657879,8899710,16267290]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|2548995], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
[protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]: autorepression, in [regulon|protein:BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|fosB]' and '[protein|search|lexA]' [PubMed|20525796]
Biological materials
Mutant
BKE17850 (Δ[gene|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCGCACCTCAAAAC, downstream forward: _UP4_TAATCTATTGATCTATCAGA
BKK17850 (Δ[gene|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCGCACCTCAAAAC, downstream forward: _UP4_TAATCTATTGATCTATCAGA
References
Reviews
Original Publications
Page visits: 7013
Time of last update: 2024-04-24 01:48:56
Author of last update: Jstuelk