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International Conference on Bacilli and Gram-positive bacteria

June 11 - 15, 2017, Berlin, Germany

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Categories containing this gene/protein

transcription factors and their control, resistance against oxidative and electrophile stress, resistance against toxins/ antibiotics, phosphoproteins

This gene is a member of the following regulons

LiaR regulon

The LiaR regulon


  • Coordinates on the chromosome (coding sequence): 3,394,422 -> 3,395,057
  • The protein

    Catalyzed reaction/ biological activity

  • regulation of the liaI-liaH-liaG-liaF-liaS-liaR operon in response to bacitracin
  • Protein family

    Paralogous protein(s)

    Kinetic information



  • phosphorylation on a Asp residue by LiaS
  • Cofactors

    Effectors of protein activity



  • cytoplasm (according to Swiss-Prot)
  • Interactions

  • LiaS-LiaR
  • Additional information

    Expression and Regulation


  • liaI-liaH-liaG-liaF-liaS-liaR PubMed
  • liaG-liaF-liaS-liaR PubMed
  • Sigma factor

  • liaI: SigA PubMed
  • liaG: SigA PubMed
  • Regulation

  • liaI: induced by bacitracin and rhamnolipids (LiaR) PubMed
  • induced by daptomycin PubMed
  • liaI: repressed during logarithmic growth (AbrB) PubMed, indirect regulation by AbrB PubMed
  • liaG: constitutive
  • Regulatory mechanism

  • LiaR: transcription activation PubMed
  • Additional information

    Biological materials


  • MGNA-B034 (yvqC::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein

  • John Helmann, Cornell University, USA Homepage
  • References


    Radeck J(1), Fritz G(2), Mascher T(3).

    The cell envelope stress response of Bacillus subtilis: from static signaling devices to dynamic regulatory network.

    Curr Genet. 2017 Feb;63(1):79-90. doi: 10.1007/s00294-016-0624-0. Epub 2016 Jun 25.

    Original publications

    Elsayed KM, Islam MR, Abdullah-Al-Mahin, Nagao JI, Zendo T, Sonomoto K

    LiaRS reporter assay: A simple tool to identify lipid II binding moieties in lantibiotic nukacin ISK-1

    J Biosci Bioeng. 2017 Mar;123(3):398-401. doi: 10.1016/j.jbiosc.2016.10.002. Epub 2016 Nov 14. PubMed PMID: 27856233.
    Müller A, Wenzel M, Strahl H, Grein F, Saaki TN, Kohl B, Siersma T, Bandow JE, Sahl HG, Schneider T, Hamoen LW

    Daptomycin inhibits cell envelope synthesis by interfering with fluid membrane microdomains

    Proc Natl Acad Sci U S A. 2016 Oct 24. pii: 201611173. [Epub ahead of print] PubMed PMID: 27791134; PubMed Central PMCID: PMC5111643.
    Kesel S, Mader A, Höfler C, Mascher T, Leisner M

    Immediate and heterogeneous response of the LiaFSR two-component system of Bacillus subtilis to the peptide antibiotic bacitracin

    PLoS One. 2013;8(1):e53457. doi: 10.1371/journal.pone.0053457. Epub 2013 Jan 11. PubMed PMID: 23326432; PubMed Central PMCID: PMC3543457.
    Schrecke K, Jordan S, Mascher T

    Stoichiometry and perturbation studies of the LiaFSR system of Bacillus subtilis

    Mol Microbiol. 2013 Feb;87(4):769-88. doi: 10.1111/mmi.12130. Epub 2013 Jan 21. PubMed PMID: 23279150.
    Wolf D, Domínguez-Cuevas P, Daniel RA, Mascher T

    Cell envelope stress response in cell wall-deficient L-forms of Bacillus subtilis

    Antimicrob Agents Chemother. 2012 Nov;56(11):5907-15. doi: 10.1128/AAC.00770-12. Epub 2012 Sep 10. PubMed PMID: 22964256; PubMed Central PMCID: PMC3486569.
    Wecke T, Bauer T, Harth H, Mäder U, Mascher T

    The rhamnolipid stress response of Bacillus subtilis

    FEMS Microbiol Lett. 2011 Oct;323(2):113-23. doi: 10.1111/j.1574-6968.2011.02367.x. Epub 2011 Aug 24. PubMed PMID: 22092710.
    Chumsakul O, Takahashi H, Oshima T, Hishimoto T, Kanaya S, Ogasawara N, Ishikawa S

    Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation

    Nucleic Acids Res. 2011 Jan;39(2):414-28. doi: 10.1093/nar/gkq780. Epub 2010 Sep 3. PubMed PMID: 20817675; PubMed Central PMCID: PMC3025583.
    Wolf D, Kalamorz F, Wecke T, Juszczak A, Mäder U, Homuth G, Jordan S, Kirstein J, Hoppert M, Voigt B, Hecker M, Mascher T

    In-depth profiling of the LiaR response of Bacillus subtilis

    J Bacteriol. 2010 Sep;192(18):4680-93. doi: 10.1128/JB.00543-10. Epub 2010 Jul 16. PubMed PMID: 20639339; PubMed Central PMCID: PMC2937411.
    Rukmana A, Morimoto T, Takahashi H, Giyanto, Ogasawara N

    Assessment of transcriptional responses of Bacillus subtilis cells to the antibiotic enduracidin, which interferes with cell wall synthesis, using a high-density tiling chip

    Genes Genet Syst. 2009 Aug;84(4):253-67. Erratum in: Genes Genet Syst. 2009 Oct;84(5):2 p following 395. PubMed PMID: 20057163.
    Wecke T, Zühlke D, Mäder U, Jordan S, Voigt B, Pelzer S, Labischinski H, Homuth G, Hecker M, Mascher T

    Daptomycin versus Friulimicin B: in-depth profiling of Bacillus subtilis cell envelope stress responses

    Antimicrob Agents Chemother. 2009 Apr;53(4):1619-23. doi: 10.1128/AAC.01046-08. Epub 2009 Jan 21. PubMed PMID: 19164157; PubMed Central PMCID: PMC2663105.
    Hachmann AB, Angert ER, Helmann JD

    Genetic analysis of factors affecting susceptibility of Bacillus subtilis to daptomycin

    Antimicrob Agents Chemother. 2009 Apr;53(4):1598-609. doi: 10.1128/AAC.01329-08. Epub 2009 Jan 21. PubMed PMID: 19164152; PubMed Central PMCID: PMC2663116.
    Rietkötter E, Hoyer D, Mascher T

    Bacitracin sensing in Bacillus subtilis

    Mol Microbiol. 2008 May;68(3):768-85. doi: 10.1111/j.1365-2958.2008.06194.x. PubMed PMID: 18394148.
    Butcher BG, Lin YP, Helmann JD

    The yydFGHIJ operon of Bacillus subtilis encodes a peptide that induces the LiaRS two-component system

    J Bacteriol. 2007 Dec;189(23):8616-25. Epub 2007 Oct 5. PubMed PMID: 17921301; PubMed Central PMCID: PMC2168931.
    Jordan S, Rietkötter E, Strauch MA, Kalamorz F, Butcher BG, Helmann JD, Mascher T

    LiaRS-dependent gene expression is embedded in transition state regulation in Bacillus subtilis

    Microbiology. 2007 Aug;153(Pt 8):2530-40. PubMed PMID: 17660417.
    Jordan S, Rietkötter E, Strauch MA, Kalamorz F, Butcher BG, Helmann JD, Mascher T

    LiaRS-dependent gene expression is embedded in transition state regulation in Bacillus subtilis

    Microbiology. 2007 Aug;153(Pt 8):2530-40. PubMed PMID: 17660417.
    Hyyryläinen HL, Pietiäinen M, Lundén T, Ekman A, Gardemeister M, Murtomäki-Repo S, Antelmann H, Hecker M, Valmu L, Sarvas M, Kontinen VP

    The density of negative charge in the cell wall influences two-component signal transduction in Bacillus subtilis

    Microbiology. 2007 Jul;153(Pt 7):2126-36. PubMed PMID: 17600057.
    Jordan S, Junker A, Helmann JD, Mascher T

    Regulation of LiaRS-dependent gene expression in bacillus subtilis: identification of inhibitor proteins, regulator binding sites, and target genes of a conserved cell envelope stress-sensing two-component system

    J Bacteriol. 2006 Jul;188(14):5153-66. PubMed PMID: 16816187; PubMed Central PMCID: PMC1539951.
    Mascher T, Zimmer SL, Smith TA, Helmann JD

    Antibiotic-inducible promoter regulated by the cell envelope stress-sensing two-component system LiaRS of Bacillus subtilis

    Antimicrob Agents Chemother. 2004 Aug;48(8):2888-96. PubMed PMID: 15273097; PubMed Central PMCID: PMC478541.
    Hamon MA, Stanley NR, Britton RA, Grossman AD, Lazazzera BA

    Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis

    Mol Microbiol. 2004 May;52(3):847-60. PubMed PMID: 15101989; PubMed Central PMCID: PMC1409746.
    1. J Bacteriol. 1999 Apr;181(7):1975-83. Two-component signal transduction in Bacillus subtilis: how one organism sees its world. Fabret C(1), Feher VA, Hoch JA. Author information: (1)Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA. PMCID: PMC93607 PMID: 10094672 [Indexed for MEDLINE]
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