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Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

The MalR regulon


  • Coordinates on the chromosome (coding sequence): 3,238,751 -> 3,239,458
  • Phenotypes of a mutant

  • unable to use malate as the single carbon source PubMed
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

    Paralogous protein(s)

    Kinetic information



  • phosphorylated by MalK on an Asp residue
  • Cofactors

    Effectors of protein activity

  • phosphorylation likely affects DNA-binding activity
  • Structure


  • cytoplasm (according to Swiss-Prot)
  • Interactions

  • MalK-MalR
  • Additional information

    Expression and Regulation


  • malR PubMed
  • Sigma factor


    Regulatory mechanism

    Additional information

    Biological materials


  • MGNA-A635 (yufM::erm), available at the NBRP B. subtilis, Japan
  • GP1301 (cat), available in Stülke lab
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein

  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
  • References

    Doan T, Servant P, Tojo S, Yamaguchi H, Lerondel G, Yoshida K, Fujita Y, Aymerich S

    The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate

    Microbiology. 2003 Sep;149(Pt 9):2331-43. PubMed PMID: 12949160.
    Tanaka K, Kobayashi K, Ogasawara N

    The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium

    Microbiology. 2003 Sep;149(Pt 9):2317-29. PubMed PMID: 12949159.
    Fabret C, Feher VA, Hoch JA

    Two-component signal transduction in Bacillus subtilis: how one organism sees its world

    J Bacteriol. 1999 Apr;181(7):1975-83. Review. PubMed PMID: 10094672; PubMed Central PMCID: PMC93607.
    Oudega B, Koningstein G, Rodrigues L, de Sales Ramon M, Hilbert H, Düsterhöft A, Pohl TM, Weitzenegger T

    Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)

    Microbiology. 1997 Aug;143 ( Pt 8):2769-74. PubMed PMID: 9274030.
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