SubtiBank SubtiBank

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Categories containing this gene/protein

translation, membrane proteins

This gene is a member of the following regulons

The MifM regulon


  • Coordinates on the chromosome (coding sequence): 2,483,586 -> 2,483,873
  • The protein

    Catalyzed reaction/ biological activity

  • acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yidC2 mRNA PubMed
  • Protein family

    Paralogous protein(s)

    Kinetic information


  • N-terminal transmembrane domain PubMed
  • the C-terminal domain interacts with the ribosome and arrests the own translation elongation PubMed
  • Modification


    Effectors of protein activity


  • EMD-6306 (MifM-stalled ribosome complex) PubMed
  • 3J9W (MifM-stalled ribosome complex) PubMed
  • Localization

  • membrane PubMed
  • Interactions

  • the C-terminus of MifM interacts with the ribosomal polypeptide exit tunnel (RplD, RplV) PubMed
  • SpoIIIJ-MifM PubMed
  • RplD-MifM PubMed
  • RplV-MifM PubMed
  • 23S rRNA-MifM PubMed
  • Additional information

    Expression and Regulation


  • mifM-yidC2 PubMed
  • Sigma factor


    Regulatory mechanism

    Additional information

  • An antisense RNA is predicted for mifM PubMed
  • MifM has intrinsic sequences to arrest ribosome function, the arrest is influenced by the cotranslational engagement in the protein localization reaction PubMed
  • elongation proceeds when SpoIIIJ integrates the nascent chain of MifM into the membrane PubMed
  • Biological materials


    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein


    Sohmen D, Chiba S, Shimokawa-Chiba N, Innis CA, Berninghausen O, Beckmann R, Ito K, Wilson DN

    Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling

    Nat Commun. 2015 Apr 23;6:6941. doi: 10.1038/ncomms7941. PubMed PMID: 25903689; PubMed Central PMCID: PMC4423224.
    Chiba S, Ito K

    MifM monitors total YidC activities of Bacillus subtilis, including that of YidC2, the target of regulation

    J Bacteriol. 2015 Jan 1;197(1):99-107. doi: 10.1128/JB.02074-14. Epub 2014 Oct 13. PubMed PMID: 25313395; PubMed Central PMCID: PMC4288694.
    Chiba S, Ito K

    Multisite ribosomal stalling: a unique mode of regulatory nascent chain action revealed for MifM

    Mol Cell. 2012 Sep 28;47(6):863-72. doi: 10.1016/j.molcel.2012.06.034. Epub 2012 Aug 2. PubMed PMID: 22864117.
    Li GW, Oh E, Weissman JS

    The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria

    Nature. 2012 Mar 28;484(7395):538-41. doi: 10.1038/nature10965. PubMed PMID: 22456704; PubMed Central PMCID: PMC3338875.
    Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P

    Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis

    Science. 2012 Mar 2;335(6072):1103-6. doi: 10.1126/science.1206848. PubMed PMID: 22383849.
    Chiba S, Kanamori T, Ueda T, Akiyama Y, Pogliano K, Ito K

    Recruitment of a species-specific translational arrest module to monitor different cellular processes

    Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):6073-8. doi: 10.1073/pnas.1018343108. Epub 2011 Mar 7. PubMed PMID: 21383133; PubMed Central PMCID: PMC3076874.
    Irnov I, Sharma CM, Vogel J, Winkler WC

    Identification of regulatory RNAs in Bacillus subtilis

    Nucleic Acids Res. 2010 Oct;38(19):6637-51. doi: 10.1093/nar/gkq454. Epub 2010 Jun 4. PubMed PMID: 20525796; PubMed Central PMCID: PMC2965217.
    Chiba S, Lamsa A, Pogliano K

    A ribosome-nascent chain sensor of membrane protein biogenesis in Bacillus subtilis

    EMBO J. 2009 Nov 18;28(22):3461-75. doi: 10.1038/emboj.2009.280. Epub 2009 Sep 24. PubMed PMID: 19779460; PubMed Central PMCID: PMC2782093.
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