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Categories containing this gene/protein

trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), transcription factors and their control, membrane proteins, sporulation/ other

This gene is a member of the following regulons

The MntR regulon


  • Coordinates on the chromosome (coding sequence): 2,543,440 -> 2,543,868
  • Phenotypes of a mutant

  • inactivation of mntR reduces sporulation efficiency to 8.9% that of wild type cells PubMed
  • highly sensitive to Mn(II) intoxication PubMed
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

    Paralogous protein(s)

    Kinetic information




  • Mn(2+) acts as co-repressor (according to PubMed)
  • Effectors of protein activity


  • 2F5D (complex with manganese), 2HYG (apo-form)
  • Localization

  • cell membrane (according to Swiss-Prot)
  • Interactions

  • active as dimer (according to PubMed)
  • Additional information

    Expression and Regulation


    Sigma factor


    Regulatory mechanism

    Additional information

    Biological materials


  • MGNA-C435 (yqhN::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein

  • John Helmann, Cornell University, USA Homepage
  • Richard Brennan, Houston, Texas, USA Homepage
  • References


    Helmann JD

    Specificity of metal sensing: iron and manganese homeostasis in Bacillus subtilis

    J Biol Chem. 2014 Oct 10;289(41):28112-20. doi: 10.1074/jbc.R114.587071. Epub 2014 Aug 26. Review. PubMed PMID: 25160631; PubMed Central PMCID: PMC4192466.
    Brantl S, Licht A

    Characterisation of Bacillus subtilis transcriptional regulators involved in metabolic processes

    Curr Protein Pept Sci. 2010 Jun;11(4):274-91. Review. PubMed PMID: 20408793.

    Original Publications

    Huang X, Shin JH, Pinochet-Barros A, Su TT, Helmann JD

    Bacillus subtilis MntR coordinates the transcriptional regulation of manganese uptake and efflux systems

    Mol Microbiol. 2017 Jan;103(2):253-268. doi: 10.1111/mmi.13554. Epub 2016 Nov 2. PubMed PMID: 27748968; PubMed Central PMCID: PMC5218975.
    Meeske AJ, Rodrigues CD, Brady J, Lim HC, Bernhardt TG, Rudner DZ

    High-Throughput Genetic Screens Identify a Large and Diverse Collection of New Sporulation Genes in Bacillus subtilis

    PLoS Biol. 2016 Jan 6;14(1):e1002341. doi: 10.1371/journal.pbio.1002341. eCollection 2016 Jan. PubMed PMID: 26735940; PubMed Central PMCID: PMC4703394.
    McGuire AM, Cuthbert BJ, Ma Z, Grauer-Gray KD, Brunjes Brophy M, Spear KA, Soonsanga S, Kliegman JI, Griner SL, Helmann JD, Glasfeld A

    Roles of the A and C sites in the manganese-specific activation of MntR

    Biochemistry. 2013 Jan 29;52(4):701-13. doi: 10.1021/bi301550t. Epub 2013 Jan 17. PubMed PMID: 23298157; PubMed Central PMCID: PMC3562352.
    Golynskiy MV, Gunderson WA, Hendrich MP, Cohen SM

    Metal binding studies and EPR spectroscopy of the manganese transport regulator MntR

    Biochemistry. 2006 Dec 26;45(51):15359-72. Epub 2006 Nov 22. PubMed PMID: 17176058; PubMed Central PMCID: PMC2561245.
    DeWitt MA, Kliegman JI, Helmann JD, Brennan RG, Farrens DL, Glasfeld A

    The conformations of the manganese transport regulator of Bacillus subtilis in its metal-free state

    J Mol Biol. 2007 Feb 2;365(5):1257-65. Epub 2006 Oct 28. PubMed PMID: 17118401; PubMed Central PMCID: PMC2877129.
    Kliegman JI, Griner SL, Helmann JD, Brennan RG, Glasfeld A

    Structural basis for the metal-selective activation of the manganese transport regulator of Bacillus subtilis

    Biochemistry. 2006 Mar 21;45(11):3493-505. PubMed PMID: 16533030; PubMed Central PMCID: PMC2586665.
    Golynskiy MV, Davis TC, Helmann JD, Cohen SM

    Metal-induced structural organization and stabilization of the metalloregulatory protein MntR

    Biochemistry. 2005 Mar 8;44(9):3380-9. PubMed PMID: 15736948.
    Lieser SA, Davis TC, Helmann JD, Cohen SM

    DNA-binding and oligomerization studies of the manganese(II) metalloregulatory protein MntR from Bacillus subtilis

    Biochemistry. 2003 Nov 4;42(43):12634-42. PubMed PMID: 14580210.
    Guedon E, Moore CM, Que Q, Wang T, Ye RW, Helmann JD

    The global transcriptional response of Bacillus subtilis to manganese involves the MntR, Fur, TnrA and sigmaB regulons

    Mol Microbiol. 2003 Sep;49(6):1477-91. PubMed PMID: 12950915.
    Glasfeld A, Guedon E, Helmann JD, Brennan RG

    Structure of the manganese-bound manganese transport regulator of Bacillus subtilis

    Nat Struct Biol. 2003 Aug;10(8):652-7. PubMed PMID: 12847518.
    Guedon E, Helmann JD

    Origins of metal ion selectivity in the DtxR/MntR family of metalloregulators

    Mol Microbiol. 2003 Apr;48(2):495-506. PubMed PMID: 12675807.
    Que Q, Helmann JD

    Manganese homeostasis in Bacillus subtilis is regulated by MntR, a bifunctional regulator related to the diphtheria toxin repressor family of proteins

    Mol Microbiol. 2000 Mar;35(6):1454-68. PubMed PMID: 10760146.
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