SubtiBank SubtiBank

Categories containing this gene/protein


cell shape, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes, membrane proteins

This gene is a member of the following regulons


SigM regulon, ComK regulon

Gene


  • Coordinates on the chromosome (coding sequence): 2,859,832 -> 2,860,704
  • Phenotypes of a mutant

  • mreC is essential under normal conditions PubMed.
  • Depletion of MreC leads to a progressive increase in the width and a decrease in the length of the cell. This shape defect is consistent with a role for mreC in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation like rodA and the redundant gene pair pbpA and pbpH.
  • Electron microscopy of cells depleted of MreC shows regions of the cell where a thick and irregular cell wall has accumulated PubMed.
  • mreC can be deleted provided that 0.5 M sucrose and 20 mM Mg(2 ) is provided in the media, mreC is therefore conditionally essential. The phenotype of the mreC deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters PubMed.
  • The protein


    Catalyzed reaction/ biological activity

  • None/ structural protein
  • Protein family

  • mreC family (according to Swiss-Prot) COG1793
  • Paralogous protein(s)

  • None
  • Kinetic information

    Domains

  • Intracellular N-terminus, transmembrane domain, Coiled coil domain and C-terminal beta-sheet domain.
  • Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 2J5U: MreC from Listeria monocytogenes PubMed
  • 2QF4: MreC monomer from Streptococcus pneumoniae PubMed
  • 2QF5: MreC dimer from Streptococcus pneumoniae PubMed
  • Localization

  • trans-membrane protein PubMed
  • during logarithmic growth, MreD forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis PubMed
  • forms transverse bands as cells enter the stationary phase PubMed
  • reports on helical structures formed by MreC PubMed seem to be misinterpretation of data PubMed
  • Interactions

  • part of the cell wall biosynthetic complex PubMed
  • MreC-MreD, interacts also with a subset of the [penicillin-binding protein] PubMed
  • MreC-GpsB PubMed
  • Additional information

    Expression and Regulation


    Operon

  • maf-radC-mreB-mreC-mreD-minC-minD PubMed
  • mreB-mreC-mreD-minC-minD PubMed
  • Sigma factor

  • promoter p1 (upstream of maf): SigA PubMed
  • promoter p2 (within maf): SigM PubMed
  • promoter p3 (upstream of mreB): SigA PubMed
  • Regulation

  • expression is reduced in a SigV mutant PubMed
  • promoter p3 (upstream of mreB): expressed during the development of genetic competence (ComK) PubMed
  • Regulatory mechanism

  • ComK: transcription activation PubMed
  • Additional information

    Biological materials


    Mutant

  • A non-polar inframe deletion strain named 3481 and a xylose dependent conditional mutant named 3461 is avaliable from the Errington lab PubMed.
  • Expression vector

    lacZ fusion

    GFP fusion

  • A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3417 PubMed.
  • two-hybrid system

    FLAG-tag construct

    Antibody

  • antisera raised in rabit is avaliable from the Errington lab.
  • Labs working on this gene/protein


  • Jeff Errington, Newcastle University, UK homepage
  • Peter Graumann, Freiburg University, Germany homepage
  • References


    Mirouze N, Ferret C, Yao Z, Chastanet A, Carballido-López R

    MreB-Dependent Inhibition of Cell Elongation during the Escape from Competence in Bacillus subtilis

    PLoS Genet. 2015 Jun 19;11(6):e1005299. doi: 10.1371/journal.pgen.1005299. eCollection 2015 Jun 19. PubMed PMID: 26091431; PubMed Central PMCID: PMC4474612.
    Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P

    Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis

    Science. 2012 Mar 2;335(6072):1103-6. doi: 10.1126/science.1206848. PubMed PMID: 22383849.
    Guariglia-Oropeza V, Helmann JD

    Bacillus subtilis σ(V) confers lysozyme resistance by activation of two cell wall modification pathways, peptidoglycan O-acetylation and D-alanylation of teichoic acids

    J Bacteriol. 2011 Nov;193(22):6223-32. doi: 10.1128/JB.06023-11. Epub 2011 Sep 16. PubMed PMID: 21926231; PubMed Central PMCID: PMC3209214.
    Garner EC, Bernard R, Wang W, Zhuang X, Rudner DZ, Mitchison T

    Coupled, circumferential motions of the cell wall synthesis machinery and MreB filaments in B

    subtilis. Science. 2011 Jul 8;333(6039):222-5. doi: 10.1126/science.1203285. Epub 2011 Jun 2. PubMed PMID: 21636745; PubMed Central PMCID: PMC3235694.
    Domínguez-Escobar J, Chastanet A, Crevenna AH, Fromion V, Wedlich-Söldner R, Carballido-López R

    Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria

    Science. 2011 Jul 8;333(6039):225-8. doi: 10.1126/science.1203466. Epub 2011 Jun 2. PubMed PMID: 21636744.
    Strahl H, Hamoen LW

    Membrane potential is important for bacterial cell division

    Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12281-6. doi: 10.1073/pnas.1005485107. Epub 2010 Jun 21. PubMed PMID: 20566861; PubMed Central PMCID: PMC2901462.
    Claessen D, Emmins R, Hamoen LW, Daniel RA, Errington J, Edwards DH

    Control of the cell elongation-division cycle by shuttling of PBP1 protein in Bacillus subtilis

    Mol Microbiol. 2008 May;68(4):1029-46. doi: 10.1111/j.1365-2958.2008.06210.x. Epub 2008 Mar 19. PubMed PMID: 18363795.
    Eiamphungporn W, Helmann JD

    The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses

    Mol Microbiol. 2008 Feb;67(4):830-48. doi: 10.1111/j.1365-2958.2007.06090.x. Epub 2008 Jan 2. PubMed PMID: 18179421; PubMed Central PMCID: PMC3025603.
    van den Ent F, Leaver M, Bendezu F, Errington J, de Boer P, Löwe J

    Dimeric structure of the cell shape protein MreC and its functional implications

    Mol Microbiol. 2006 Dec;62(6):1631-42. PubMed PMID: 17427287.
    Leaver M, Errington J

    Roles for MreC and MreD proteins in helical growth of the cylindrical cell wall in Bacillus subtilis

    Mol Microbiol. 2005 Sep;57(5):1196-209. PubMed PMID: 16101995.
    Lee S, Price CW

    The minCD locus of Bacillus subtilis lacks the minE determinant that provides topological specificity to cell division

    Mol Microbiol. 1993 Feb;7(4):601-10. PubMed PMID: 8459776.
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