SubtiBank SubtiBank

Categories containing this gene/protein

coping with hypo-osmotic stress, membrane proteins

This gene is a member of the following regulons


  • Coordinates on the chromosome (coding sequence): 3,743,267 -> 3,743,659
  • Phenotypes of a mutant

  • sensitive to osmotic downshock (> 0.5 M) during logarithmic growth PubMed
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

  • mscL family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information




    Effectors of protein activity


  • 3HZQ (MscL of Staphylococcus aureus) PubMed
  • Localization

  • cell membrane PubMed
  • Interactions

    Additional information

    Expression and Regulation


  • mscL PubMed
  • Sigma factor


  • expressed in logarithmic phase PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


  • 1A957 ( mscL::spec), PubMed, available at BGSC
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein

  • Jan Maarten van Dijl, Groningen, Netherlands
  • Erhard Bremer, University of Marburg, Germany homepage
  • References


    Reuter M, Hayward NJ, Black SS, Miller S, Dryden DT, Booth IR

    Mechanosensitive channels and bacterial cell wall integrity: does life end with a bang or a whimper

    J R Soc Interface. 2013 Nov 20;11(91):20130850. doi: 10.1098/rsif.2013.0850. Print 2014 Feb 6. PubMed PMID: 24258154; PubMed Central PMCID: PMC3869158.
    Booth IR, Blount P

    The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves

    J Bacteriol. 2012 Sep;194(18):4802-9. doi: 10.1128/JB.00576-12. Epub 2012 Jun 8. Review. PubMed PMID: 22685280; PubMed Central PMCID: PMC3430326.
    Kung C, Martinac B, Sukharev S

    Mechanosensitive channels in microbes

    Annu Rev Microbiol. 2010;64:313-29. doi: 10.1146/annurev.micro.112408.134106. Review. PubMed PMID: 20825352.
    Vásquez V, Perozo E

    Structural biology: A channel with a twist

    Nature. 2009 Sep 3;461(7260):47-9. doi: 10.1038/461047a. PubMed PMID: 19727188.
    Pivetti CD, Yen MR, Miller S, Busch W, Tseng YH, Booth IR, Saier MH Jr

    Two families of mechanosensitive channel proteins

    Microbiol Mol Biol Rev. 2003 Mar;67(1):66-85, table of contents. Review. PubMed PMID: 12626684; PubMed Central PMCID: PMC150521.

    Original Publications

    Kouwen TR, Trip EN, Denham EL, Sibbald MJ, Dubois JY, van Dijl JM

    The large mechanosensitive channel MscL determines bacterial susceptibility to the bacteriocin sublancin 168

    Antimicrob Agents Chemother. 2009 Nov;53(11):4702-11. doi: 10.1128/AAC.00439-09. Epub 2009 Sep 8. PubMed PMID: 19738010; PubMed Central PMCID: PMC2772344.
    Liu Z, Gandhi CS, Rees DC

    Structure of a tetrameric MscL in an expanded intermediate state

    Nature. 2009 Sep 3;461(7260):120-4. doi: 10.1038/nature08277. Epub 2009 Aug 23. PubMed PMID: 19701184; PubMed Central PMCID: PMC2737600.
    Wahome PG, Cowan AE, Setlow B, Setlow P

    Levels and localization of mechanosensitive channel proteins in Bacillus subtilis

    Arch Microbiol. 2009 May;191(5):403-14. doi: 10.1007/s00203-009-0465-z. Epub 2009 Feb 28. PubMed PMID: 19252899.
    Kouwen TR, Antelmann H, van der Ploeg R, Denham EL, Hecker M, van Dijl JM

    MscL of Bacillus subtilis prevents selective release of cytoplasmic proteins in a hypotonic environment

    Proteomics. 2009 Feb;9(4):1033-43. doi: 10.1002/pmic.200800483. PubMed PMID: 19160392.
    Hoffmann T, Boiangiu C, Moses S, Bremer E

    Responses of Bacillus subtilis to hypotonic challenges: physiological contributions of mechanosensitive channels to cellular survival

    Appl Environ Microbiol. 2008 Apr;74(8):2454-60. doi: 10.1128/AEM.01573-07. Epub 2008 Feb 29. PubMed PMID: 18310427; PubMed Central PMCID: PMC2293136.
    Wahome PG, Setlow P

    Growth, osmotic downshock resistance and differentiation of Bacillus subtilis strains lacking mechanosensitive channels

    Arch Microbiol. 2008 Jan;189(1):49-58. Epub 2007 Jul 31. PubMed PMID: 17665170.
    Wahome PG, Setlow P

    The synthesis and role of the mechanosensitive channel of large conductance in growth and differentiation of Bacillus subtilis

    Arch Microbiol. 2006 Nov;186(5):377-83. Epub 2006 Aug 5. PubMed PMID: 16897034.
    Moe PC, Blount P, Kung C

    Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation

    Mol Microbiol. 1998 May;28(3):583-92. PubMed PMID: 9632260.
    Presecan E, Moszer I, Boursier L, Cruz Ramos HC, de la Fuente V, Hullo MF, Lelong C, Schleich S, Sekowska A, Song BH, Villani G, Kunst F, Danchin A, Glaser P

    The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)

    Microbiology. 1997 Oct;143 ( Pt 10):3313-28. PubMed PMID: 9353933.
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