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Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control

This gene is a member of the following regulons

NagR regulon

The NagR regulon


  • Coordinates on the chromosome (coding sequence): 3,597,289 -> 3,598,020
  • The protein

    Catalyzed reaction/ biological activity

  • transcription repression of nagP and of the putative nagA-nagB-nagR operon in the absence of N-acetylglycosamine PubMed, weak repression of yflG PubMed
  • Protein family

  • GntR family of transcription factors
  • Paralogous protein(s)

    Kinetic information

  • K(D) for N-acetylglucosamine-6-phosphate: 1 mM PubMed
  • Domains



    Effectors of protein activity

  • N-acetylglucosamine-6-phosphate and glucosamine-6-phosphate act as the molecular inducers PubMed
  • Structure

  • 2WV0 PubMed
  • 4U0V (complex with glucosamine-6-phosphate) PubMed
  • 4U0W (complex with N-acetylglucosamine-6-phosphate) PubMed
  • 4WWC (complex with palindromic operator DNA) PubMed
  • Localization


    Additional information

    Expression and Regulation


  • nagA-nagB-nagR PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • induced in the presence of N-acetylglucosamine (NagR) PubMed
  • Regulatory mechanism

  • NagR: transcription repression PubMed
  • Additional information

    Biological materials


  • MGNA-A335 (yvoA::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein



    Plumbridge J(1).

    Regulation of the Utilization of Amino Sugars by Escherichia coli and Bacillus subtilis: Same Genes, Different Control.

    J Mol Microbiol Biotechnol. 2015;25(2-3):154-67. doi: 10.1159/000369583. Epub 2015 Jul 9.DOI: 10.1159/000369583 PMID: 26159076 [Indexed for MEDLINE]

    Original publications

    Fillenberg SB, Grau FC, Seidel G, Muller YA

    Structural insight into operator dre-sites recognition and effector binding in the GntR/HutC transcription regulator NagR

    Nucleic Acids Res. 2015 Jan;43(2):1283-96. doi: 10.1093/nar/gku1374. Epub 2015 Jan 6. PubMed PMID: 25564531; PubMed Central PMCID: PMC4333415.
    Gaugué I, Oberto J, Plumbridge J

    Regulation of amino sugar utilization in Bacillus subtilis by the GntR family regulators, NagR and GamR

    Mol Microbiol. 2014 Apr;92(1):100-15. doi: 10.1111/mmi.12544. Epub 2014 Mar 5. PubMed PMID: 24673833.
    Gaugué I, Oberto J, Putzer H, Plumbridge J

    The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine

    PLoS One. 2013 May 8;8(5):e63025. doi: 10.1371/journal.pone.0063025. Print 2013. PubMed PMID: 23667565; PubMed Central PMCID: PMC3648570.
    Bertram R, Rigali S, Wood N, Lulko AT, Kuipers OP, Titgemeyer F

    Regulon of the N-acetylglucosamine utilization regulator NagR in Bacillus subtilis

    J Bacteriol. 2011 Jul;193(14):3525-36. doi: 10.1128/JB.00264-11. Epub 2011 May 20. PubMed PMID: 21602348; PubMed Central PMCID: PMC3133301.
    Resch M, Schiltz E, Titgemeyer F, Muller YA

    Insight into the induction mechanism of the GntR/HutC bacterial transcription regulator YvoA

    Nucleic Acids Res. 2010 Apr;38(7):2485-97. doi: 10.1093/nar/gkp1191. Epub 2010 Jan 4. PubMed PMID: 20047956; PubMed Central PMCID: PMC2853113.
    Resch M, Roth HM, Kottmair M, Sevvana M, Bertram R, Titgemeyer F, Muller YA

    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of YvoA from Bacillus subtilis

    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Apr 1;65(Pt 4):410-4. doi: 10.1107/S1744309109008628. Epub 2009 Mar 26. PubMed PMID: 19342794; PubMed Central PMCID: PMC2664774.
    You C, Lu H, Sekowska A, Fang G, Wang Y, Gilles AM, Danchin A

    The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis

    BMC Microbiol. 2005 Oct 5;5:57. PubMed PMID: 16207374; PubMed Central PMCID: PMC1266368.
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