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Categories containing this gene/protein

transcription factors and their control, resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

This gene is a member of the following regulons

The NsrR regulon


  • Coordinates on the chromosome (coding sequence): 1,013,411 -> 1,013,851
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

    Paralogous protein(s)

    Kinetic information




  • [4Fe-4S] cluster, required for DNA-binding PubMed
  • Effectors of protein activity

  • nitric oxide (NO) acts as molecular inducer and results in NsrR release from its DNA targets PubMed
  • [4Fe-4S]-NsrR binds around the -35 element of the nasD promoter with much higher affinity than apo-NsrR and binding of [4Fe-4S]-NsrR, but not apo-protein, is sensitive to NO PubMed
  • Structure



    Additional information

    Expression and Regulation


  • nsrR PubMed
  • Sigma factor


    Regulatory mechanism

    Additional information

    Biological materials


  • MGNA-A692 (yhdE::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein



    Härtig E, Jahn D

    Regulation of the anaerobic metabolism in Bacillus subtilis

    Adv Microb Physiol. 2012;61:195-216. doi: 10.1016/B978-0-12-394423-8.00005-6. Review. PubMed PMID: 23046954.
    Tucker NP, Le Brun NE, Dixon R, Hutchings MI

    There's NO stopping NsrR, a global regulator of the bacterial NO stress response

    Trends Microbiol. 2010 Apr;18(4):149-56. doi: 10.1016/j.tim.2009.12.009. Epub 2010 Feb 16. Review. PubMed PMID: 20167493.
    Rodionov DA, Dubchak IL, Arkin AP, Alm EJ, Gelfand MS

    Dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks

    PLoS Comput Biol. 2005 Oct;1(5):e55. Epub 2005 Oct 28. PubMed PMID: 16261196; PubMed Central PMCID: PMC1274295.

    The NsrR regulon

    Kommineni S(1), Lama A, Popescu B, Nakano MM.

    Global transcriptional control by NsrR in Bacillus subtilis.

    J Bacteriol. 2012 Apr;194(7):1679-88. doi: 10.1128/JB.06486-11. Epub 2012 Jan 27.

    Other original publications

    Henares B, Kommineni S, Chumsakul O, Ogasawara N, Ishikawa S, Nakano MM

    The ResD response regulator, through functional interaction with NsrR and fur, plays three distinct roles in Bacillus subtilis transcriptional control

    J Bacteriol. 2014 Jan;196(2):493-503. doi: 10.1128/JB.01166-13. Epub 2013 Nov 8. PubMed PMID: 24214949; PubMed Central PMCID: PMC3911253.
    Kommineni S, Yukl E, Hayashi T, Delepine J, Geng H, Moënne-Loccoz P, Nakano MM

    Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter

    Mol Microbiol. 2010 Dec;78(5):1280-93. doi: 10.1111/j.1365-2958.2010.07407.x. Epub 2010 Oct 8. PubMed PMID: 21091510; PubMed Central PMCID: PMC3075490.
    Yukl ET, Elbaz MA, Nakano MM, Moënne-Loccoz P

    Transcription Factor NsrR from Bacillus subtilis Senses Nitric Oxide with a 4Fe-4S Cluster (†)

    Biochemistry. 2008 Dec 9;47(49):13084-92. doi: 10.1021/bi801342x. PubMed PMID: 19006327; PubMed Central PMCID: PMC2891187.
    Tucker NP, Hicks MG, Clarke TA, Crack JC, Chandra G, Le Brun NE, Dixon R, Hutchings MI

    The transcriptional repressor protein NsrR senses nitric oxide directly via a [2Fe-2S] cluster

    PLoS One. 2008;3(11):e3623. doi: 10.1371/journal.pone.0003623. Epub 2008 Nov 7. PubMed PMID: 18989365; PubMed Central PMCID: PMC2577008.
    Rogstam A, Larsson JT, Kjelgaard P, von Wachenfeldt C

    Mechanisms of adaptation to nitrosative stress in Bacillus subtilis

    J Bacteriol. 2007 Apr;189(8):3063-71. Epub 2007 Feb 9. PubMed PMID: 17293416; PubMed Central PMCID: PMC1855868.
    Nakano MM, Geng H, Nakano S, Kobayashi K

    The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression

    J Bacteriol. 2006 Aug;188(16):5878-87. PubMed PMID: 16885456; PubMed Central PMCID: PMC1540067.
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