nth
168
apurinic/apyrimidinic endonuclease , endonuclease III, removes abasic sites during base excision repair
locus
BSU_22340
Molecular weight
24.85 kDa
pI
9.28
function
DNA repair
product
endonuclease III
essential
no
synonyms
nth, jooB, ypoB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0177 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,344,755 → 2,345,414
Phenotypes of a mutant
the mutant is sensititive to exposure to H2O2 [Pubmed|21954439]
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The protein
Catalyzed reaction/ biological activity
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to UniProt)
Protein family
Nth/MutY family (with [protein|B62083CF18FC4C796D95B960CF62FA963FC26CDE|mutY], according to UniProt)
[wiki|Domains]
HhH domain (aa 109-128) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|1P59] (complex with DNA, Geobacillus stearothermophilus)
[AF|P39788]
Effectors of protein activity
activity is stimulated by untwisting of the DNA (by [protein|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD], [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|hbs], or [protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]) [Pubmed|21954439]
Expression and Regulation
Operons
genes
[gene|FBA7EAA70F5C9E76463B0C5F85D65D4314FFD40D|asnS]-[gene|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]-[gene|4B2A124B726393544A47791E5A5681F7B766A588|ypoC]
description
[Pubmed|22383849]
genes
[gene|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]
description
[Pubmed|21954439]
regulation
constitutively expressed [Pubmed|21954439]
Biological materials
Mutant
BKE22340 (Δ[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAACACGATTGTCACCTTTT, downstream forward: _UP4_GATAAAAAAGGACTGGTGAA
BKK22340 (Δ[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAACACGATTGTCACCTTTT, downstream forward: _UP4_GATAAAAAAGGACTGGTGAA
References
Reviews
Original Publications
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Time of last update: 2024-04-14 05:51:34
Author of last update: Melvin.boenninger