SubtiBank SubtiBank

Categories containing this gene/protein

cell wall synthesis, penicillin-binding proteins

This gene is a member of the following regulons


  • Coordinates on the chromosome (coding sequence): 2,581,771 -> 2,583,921
  • The protein

    Catalyzed reaction/ biological activity

  • required for cell wall synthesis during cell elongation PubMed
  • driver of MreB dynamics PubMed
  • Protein family

    Paralogous protein(s)

    Kinetic information




    Effectors of protein activity



  • extracellular (signal peptide) PubMed
  • localization depends on the availability of peptidoglyan precursors (lipid II) PubMed
  • Interactions

  • part of the elongasome PubMed
  • folding requires PrsA PubMed
  • Additional information

    Expression and Regulation


  • pbpA PubMed
  • Sigma factor


    Regulatory mechanism

    Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • Biological materials


    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
  • FLAG-tag construct


    Labs working on this gene/protein

  • Jeff Errington, Newcastle University, UK homepage
  • References

    Lages MC, Beilharz K, Morales Angeles D, Veening JW, Scheffers DJ

    The localization of key Bacillus subtilis penicillin binding proteins during cell growth is determined by substrate availability

    Environ Microbiol. 2013 Dec;15(12):3272-81. doi: 10.1111/1462-2920.12206. Epub 2013 Jul 30. PubMed PMID: 23895585.
    Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J

    RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y

    Mol Microbiol. 2011 Sep;81(6):1459-73. doi: 10.1111/j.1365-2958.2011.07777.x. Epub 2011 Aug 4. PubMed PMID: 21815947.
    Garner EC, Bernard R, Wang W, Zhuang X, Rudner DZ, Mitchison T

    Coupled, circumferential motions of the cell wall synthesis machinery and MreB filaments in B

    subtilis. Science. 2011 Jul 8;333(6039):222-5. doi: 10.1126/science.1203285. Epub 2011 Jun 2. PubMed PMID: 21636745; PubMed Central PMCID: PMC3235694.
    Domínguez-Escobar J, Chastanet A, Crevenna AH, Fromion V, Wedlich-Söldner R, Carballido-López R

    Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria

    Science. 2011 Jul 8;333(6039):225-8. doi: 10.1126/science.1203466. Epub 2011 Jun 2. PubMed PMID: 21636744.
    Hyyryläinen HL, Marciniak BC, Dahncke K, Pietiäinen M, Courtin P, Vitikainen M, Seppala R, Otto A, Becher D, Chapot-Chartier MP, Kuipers OP, Kontinen VP

    Penicillin-binding protein folding is dependent on the PrsA peptidyl-prolyl cis-trans isomerase in Bacillus subtilis

    Mol Microbiol. 2010 Jul 1;77(1):108-27. doi: 10.1111/j.1365-2958.2010.07188.x. Epub 2010 May 4. PubMed PMID: 20487272.
    Voigt B, Antelmann H, Albrecht D, Ehrenreich A, Maurer KH, Evers S, Gottschalk G, van Dijl JM, Schweder T, Hecker M

    Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis

    J Mol Microbiol Biotechnol. 2009;16(1-2):53-68. doi: 10.1159/000142894. Epub 2008 Oct 29. PubMed PMID: 18957862.
    Wei Y, Havasy T, McPherson DC, Popham DL

    Rod shape determination by the Bacillus subtilis class B penicillin-binding proteins encoded by pbpA and pbpH

    J Bacteriol. 2003 Aug;185(16):4717-26. PubMed PMID: 12896990; PubMed Central PMCID: PMC166473.
    Murray T, Popham DL, Pearson CB, Hand AR, Setlow P

    Analysis of outgrowth of Bacillus subtilis spores lacking penicillin-binding protein 2a

    J Bacteriol. 1998 Dec;180(24):6493-502. PubMed PMID: 9851991; PubMed Central PMCID: PMC107750.
    Murray T, Popham DL, Setlow P

    Identification and characterization of pbpA encoding Bacillus subtilis penicillin-binding protein 2A

    J Bacteriol. 1997 May;179(9):3021-9. PubMed PMID: 9139922; PubMed Central PMCID: PMC179068.
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