SubtiBank SubtiBank

Categories containing this gene/protein

cell wall synthesis, cell division, essential genes, membrane proteins, penicillin-binding proteins

This gene is a member of the following regulons


  • Coordinates on the chromosome (coding sequence): 1,581,947 -> 1,584,097
  • Phenotypes of a mutant

  • essential PubMed
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

  • transpeptidase family (according to Swiss-Prot)
  • Paralogous protein(s)

  • SpoVD
  • Kinetic information


  • contains two C-terminal PASTA domains (aa 598-657, 658-714)
  • Modification


    Effectors of protein activity


  • 1RP5 (PBP 2x from Streptococcus pneumoniae, 32% identity) PubMed
  • Localization

  • cell membrane PubMed
  • localizes to the division site, this depends on FtsW PubMed
  • extracellular (signal peptide) PubMed
  • localizes to the sporulation septum during sporulation PubMed
  • Interactions

  • folding requires PrsA PubMed
  • PbpB-DivIB-(FtsL-DivIC)2 PubMed, as part of the divisome
  • WalK-PbpB PubMed
  • Additional information

    Expression and Regulation


  • mraZ-mraW-ftsL-pbpB PubMed
  • Sigma factor


  • constitutively expressed PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
  • FLAG-tag construct

  • GP2003 pbpB-3xFLAG spec (based on pGP1331), available in Jörg Stülke's lab
  • Antibody

    Labs working on this gene/protein

  • Jeff Errington, Newcastle University, UK homepage
  • References

    Gamba P, Hamoen LW, Daniel RA

    Cooperative Recruitment of FtsW to the Division Site of Bacillus subtilis

    Front Microbiol. 2016 Nov 15;7:1808. PubMed PMID: 27895631; PubMed Central PMCID: PMC5108771.
    Peters K, Schweizer I, Beilharz K, Stahlmann C, Veening JW, Hakenbeck R, Denapaite D

    Streptococcus pneumoniae PBP2x mid-cell localization requires the C-terminal PASTA domains and is essential for cell shape maintenance

    Mol Microbiol. 2014 May;92(4):733-55. doi: 10.1111/mmi.12588. PubMed PMID: 24655324.
    Lages MC, Beilharz K, Morales Angeles D, Veening JW, Scheffers DJ

    The localization of key Bacillus subtilis penicillin binding proteins during cell growth is determined by substrate availability

    Environ Microbiol. 2013 Dec;15(12):3272-81. doi: 10.1111/1462-2920.12206. PubMed PMID: 23895585.
    Fukushima T, Furihata I, Emmins R, Daniel RA, Hoch JA, Szurmant H

    A role for the essential YycG sensor histidine kinase in sensing cell division

    Mol Microbiol. 2011 Jan;79(2):503-22. doi: 10.1111/j.1365-2958.2010.07464.x. PubMed PMID: 21219466; PubMed Central PMCID: PMC3556490.
    Rowland SL, Wadsworth KD, Robson SA, Robichon C, Beckwith J, King GF

    Evidence from artificial septal targeting and site-directed mutagenesis that residues in the extracytoplasmic β domain of DivIB mediate its interaction with the divisomal transpeptidase PBP 2B

    J Bacteriol. 2010 Dec;192(23):6116-25. doi: 10.1128/JB.00783-10. PubMed PMID: 20870765; PubMed Central PMCID: PMC2981201.
    Hyyryläinen HL, Marciniak BC, Dahncke K, Pietiäinen M, Courtin P, Vitikainen M, Seppala R, Otto A, Becher D, Chapot-Chartier MP, Kuipers OP, Kontinen VP

    Penicillin-binding protein folding is dependent on the PrsA peptidyl-prolyl cis-trans isomerase in Bacillus subtilis

    Mol Microbiol. 2010 Jul 1;77(1):108-27. doi: 10.1111/j.1365-2958.2010.07188.x. PubMed PMID: 20487272.
    Gamba P, Veening JW, Saunders NJ, Hamoen LW, Daniel RA

    Two-step assembly dynamics of the Bacillus subtilis divisome

    J Bacteriol. 2009 Jul;191(13):4186-94. doi: 10.1128/JB.01758-08. PubMed PMID: 19429628; PubMed Central PMCID: PMC2698510.
    Voigt B, Antelmann H, Albrecht D, Ehrenreich A, Maurer KH, Evers S, Gottschalk G, van Dijl JM, Schweder T, Hecker M

    Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis

    J Mol Microbiol Biotechnol. 2009;16(1-2):53-68. doi: 10.1159/000142894. PubMed PMID: 18957862.
    Pernot L, Chesnel L, Le Gouellec A, Croizé J, Vernet T, Dideberg O, Dessen A

    A PBP2x from a clinical isolate of Streptococcus pneumoniae exhibits an alternative mechanism for reduction of susceptibility to beta-lactam antibiotics

    J Biol Chem. 2004 Apr 16;279(16):16463-70. PubMed PMID: 14734544.
    Scheffers DJ, Jones LJ, Errington J

    Several distinct localization patterns for penicillin-binding proteins in Bacillus subtilis

    Mol Microbiol. 2004 Feb;51(3):749-64. PubMed PMID: 14731276.
    Daniel RA, Harry EJ, Errington J

    Role of penicillin-binding protein PBP 2B in assembly and functioning of the division machinery of Bacillus subtilis

    Mol Microbiol. 2000 Jan;35(2):299-311. PubMed PMID: 10652091.
    Daniel RA, Williams AM, Errington J

    A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis

    J Bacteriol. 1996 Apr;178(8):2343-50. PubMed PMID: 8636036; PubMed Central PMCID: PMC177943.
    Yanouri A, Daniel RA, Errington J, Buchanan CE

    Cloning and sequencing of the cell division gene pbpB, which encodes penicillin-binding protein 2B in Bacillus subtilis

    J Bacteriol. 1993 Dec;175(23):7604-16. PubMed PMID: 8244929; PubMed Central PMCID: PMC206916.
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