SubtiBank SubtiBank

Categories containing this gene/protein


carbon core metabolism

This gene is a member of the following regulons


CcpN regulon

Gene


  • Coordinates on the chromosome (coding sequence): 3,129,530 -> 3,131,113
  • The protein


    Catalyzed reaction/ biological activity

  • ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2 (according to Swiss-Prot) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
  • Protein family

  • phosphoenolpyruvate carboxykinase [ATP] family (according to Swiss-Prot) phosphoenolpyruvate carboxykinase [ATP] family
  • Paralogous protein(s)

    Kinetic information

    Domains

  • Nucleotide binding Domain (233–240)
  • Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 2PXZ (E.coli)
  • Localization

  • cytoplasm (according to Swiss-Prot), cytoplasm
  • Interactions

  • Mdh-PckA (this interaction occurs under gluconeogenic, but not under glycolytic conditions) PubMed
  • Additional information

    Expression and Regulation


    Operon

  • pckA PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • repressed by glucose (35-fold) CcpN PubMed
  • Regulatory mechanism

  • CcpN: transcription repression PubMed
  • Additional information

    Biological materials


    Mutant

  • 1A1005 ( pckA::spec), PubMed, available at BGSC
  • 1A996 ( pckA::spec), PubMed, available at BGSC
  • GP1147 (pckA::neo), available in Jörg Stülke's lab
  • Expression vector

  • pGP1753 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab)
  • pGP1762 (for expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab)
  • pGP1763 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab)
  • lacZ fusion

    GFP fusion

  • GP1430 (spc, based on pGP1870), available in the Stülke lab
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

  • GP1129 (spc, based on pGP1331), available in Jörg Stülke's lab
  • Antibody

    Labs working on this gene/protein


  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
  • References


    Meyer FM, Stülke J

    Malate metabolism in Bacillus subtilis: distinct roles for three classes of malate-oxidizing enzymes

    FEMS Microbiol Lett. 2013 Feb;339(1):17-22. doi: 10.1111/1574-6968.12041. Epub 2012 Nov 28. PubMed PMID: 23136871.
    Ferguson ML, Le Coq D, Jules M, Aymerich S, Radulescu O, Declerck N, Royer CA

    Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states

    Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):155-60. doi: 10.1073/pnas.1110541108. Epub 2011 Dec 21. PubMed PMID: 22190493; PubMed Central PMCID: PMC3252923.
    Meyer FM, Gerwig J, Hammer E, Herzberg C, Commichau FM, Völker U, Stülke J

    Physical interactions between tricarboxylic acid cycle enzymes in Bacillus subtilis: evidence for a metabolon

    Metab Eng. 2011 Jan;13(1):18-27. doi: 10.1016/j.ymben.2010.10.001. Epub 2010 Oct 8. PubMed PMID: 20933603.
    Tännler S, Fischer E, Le Coq D, Doan T, Jamet E, Sauer U, Aymerich S

    CcpN controls central carbon fluxes in Bacillus subtilis

    J Bacteriol. 2008 Sep;190(18):6178-87. doi: 10.1128/JB.00552-08. Epub 2008 Jun 27. PubMed PMID: 18586936; PubMed Central PMCID: PMC2546806.
    Servant P, Le Coq D, Aymerich S

    CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes

    Mol Microbiol. 2005 Mar;55(5):1435-51. PubMed PMID: 15720552.
    Blencke HM, Homuth G, Ludwig H, Mäder U, Hecker M, Stülke J

    Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways

    Metab Eng. 2003 Apr;5(2):133-49. PubMed PMID: 12850135.
    Lapidus A, Galleron N, Sorokin A, Ehrlich SD

    Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region

    Microbiology. 1997 Nov;143 ( Pt 11):3431-41. PubMed PMID: 9387221.
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