SubtiBank SubtiBank

Categories containing this gene/protein


carbon core metabolism, phosphoproteins

This gene is a member of the following regulons


Gene


  • Coordinates on the chromosome (coding sequence): 2,986,588 -> 2,987,547
  • Phenotypes of a mutant

  • essential according to PubMed, non-essential according to PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)
  • Protein family

  • phosphofructokinase family (according to Swiss-Prot) phosphofructokinase family
  • Paralogous protein(s)

    Kinetic information

  • Allosteric Regulation (Reversible) PubMed
  • Domains

  • 3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)
  • Modification

  • phosphorylated on Arg-233 PubMed
  • Cofactors

  • Mg2+
  • Effectors of protein activity

  • Inhibited by citrate, PEP (Hill Coefficient 3) and Ca2+ (competes with Mg2+) in B. licheniformes PubMed.
  • Inhibited by ATP (competitively) and f6p (non-competitively) in G. stearothermophillus PubMed
  • Activated by GDP and ADP in lower concentrations (1mM); above that inhibition, competing with the ATP for the binding site (in G. stearothermophillus) PubMed
  • Activated by NH4+ PubMed
  • Structure

  • 4A3S PubMed
  • 1MTO (mutant, complex with fructose-6-phosphate, Geobacillus stearothermophilus)
  • 4PFK (Geobacillus stearothermophilus)
  • Localization

  • cytoplasm (Homogeneous) PubMed
  • Interactions

  • forms homotetramers PubMed
  • part of the RNA degradosome PubMed
  • PfkA-Pgm PubMed
  • PfkA-Eno PubMed, K(D) of the interaction: 40 nM PubMed
  • PfkA-Rny PubMed
  • PfkA-PnpA PubMed
  • PfkA-RnjA PubMed,
  • Additional information

  • PfkA is a moonlighting protein. PubMed
  • extensive information on the structure and enzymatic properties of PfkA can be found at Proteopedia
  • Expression and Regulation


    Operon

  • pfkA-pyk-ytzA PubMed
  • Sigma factor

    Regulation

  • twofold induced by glucose PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • GP590 (pfkA::cat), available in Jörg Stülke's lab, PubMed
  • GP595 (pfkA::erm), available in Jörg Stülke's lab, PubMed
  • GP1744: pfkA::aphA3, available in Jörg Stülke' lab
  • Expression vector

  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP87, available in Jörg Stülke's lab
  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1266, available in Jörg Stülke's lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP393, available in Jörg Stülke's lab
  • for expression in B. subtilis, in pBQ200: pGP1422, available in Jörg Stülke's lab
  • lacZ fusion

  • pGP511 (in pAC6), available in Jörg Stülke's lab
  • GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

  • GP1019 (spc, based on pGP1331), available in Jörg Stülke's lab
  • Antibody

    Labs working on this gene/protein


  • Jörg Stülke, University of Göttingen, Germany Homepage
  • References


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