SubtiBank SubtiBank

Categories containing this gene/protein


carbon core metabolism, phosphoproteins

This gene is a member of the following regulons


Gene


  • Coordinates on the chromosome (coding sequence): 2,986,588 -> 2,987,547
  • Phenotypes of a mutant

  • essential according to PubMed, non-essential according to PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)
  • Protein family

  • phosphofructokinase family (according to Swiss-Prot) phosphofructokinase family
  • Paralogous protein(s)

    Kinetic information

  • Allosteric Regulation (Reversible) PubMed
  • Domains

  • 3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)
  • Modification

  • phosphorylated on Arg-233 PubMed
  • Cofactors

  • Mg2+
  • Effectors of protein activity

  • Inhibited by citrate, PEP (Hill Coefficient 3) and Ca2+ (competes with Mg2+) in B. licheniformes PubMed.
  • Inhibited by ATP (competitively) and f6p (non-competitively) in G. stearothermophillus PubMed
  • Activated by GDP and ADP in lower concentrations (1mM); above that inhibition, competing with the ATP for the binding site (in G. stearothermophillus) PubMed
  • Activated by NH4+ PubMed
  • Structure

  • 4A3S PubMed
  • 1MTO (mutant, complex with fructose-6-phosphate, Geobacillus stearothermophilus)
  • 4PFK (Geobacillus stearothermophilus)
  • Localization

  • cytoplasm (Homogeneous) PubMed
  • Interactions

  • forms homotetramers PubMed
  • part of the RNA degradosome PubMed
  • PfkA-Pgm PubMed
  • PfkA-Eno PubMed, K(D) of the interaction: 40 nM PubMed
  • PfkA-Rny PubMed
  • PfkA-PnpA PubMed
  • PfkA-RnjA PubMed,
  • Additional information

  • PfkA is a moonlighting protein. PubMed
  • extensive information on the structure and enzymatic properties of PfkA can be found at Proteopedia
  • Expression and Regulation


    Operon

  • pfkA-pyk-ytzA PubMed
  • Sigma factor

    Regulation

  • twofold induced by glucose PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • GP590 (pfkA::cat), available in Jörg Stülke's lab, PubMed
  • GP595 (pfkA::erm), available in Jörg Stülke's lab, PubMed
  • GP1744: pfkA::aphA3, available in Jörg Stülke' lab
  • Expression vector

  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP87, available in Jörg Stülke's lab
  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1266, available in Jörg Stülke's lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP393, available in Jörg Stülke's lab
  • for expression in B. subtilis, in pBQ200: pGP1422, available in Jörg Stülke's lab
  • lacZ fusion

  • pGP511 (in pAC6), available in Jörg Stülke's lab
  • GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

  • GP1019 (spc, based on pGP1331), available in Jörg Stülke's lab
  • Antibody

    Labs working on this gene/protein


  • Jörg Stülke, University of Göttingen, Germany Homepage
  • References


    Commichau FM, Pietack N, Stülke J

    Essential genes in Bacillus subtilis: a re-evaluation after ten years

    Mol Biosyst. 2013 Jun;9(6):1068-75. doi: 10.1039/c3mb25595f. Epub 2013 Feb 18. Review. PubMed PMID: 23420519.
    Elsholz AK, Turgay K, Michalik S, Hessling B, Gronau K, Oertel D, Mäder U, Bernhardt J, Becher D, Hecker M, Gerth U

    Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis

    Proc Natl Acad Sci U S A. 2012 May 8;109(19):7451-6. doi: 10.1073/pnas.1117483109. Epub 2012 Apr 19. PubMed PMID: 22517742; PubMed Central PMCID: PMC3358850.
    Newman JA, Hewitt L, Rodrigues C, Solovyova AS, Harwood CR, Lewis RJ

    Dissection of the network of interactions that links RNA processing with glycolysis in the Bacillus subtilis degradosome

    J Mol Biol. 2012 Feb 10;416(1):121-36. doi: 10.1016/j.jmb.2011.12.024. Epub 2011 Dec 16. PubMed PMID: 22198292.
    Newman JA, Hewitt L, Rodrigues C, Solovyova AS, Harwood CR, Lewis RJ

    Dissection of the network of interactions that links RNA processing with glycolysis in the Bacillus subtilis degradosome

    J Mol Biol. 2012 Feb 10;416(1):121-36. doi: 10.1016/j.jmb.2011.12.024. Epub 2011 Dec 16. PubMed PMID: 22198292.
    Lehnik-Habrink M, Newman J, Rothe FM, Solovyova AS, Rodrigues C, Herzberg C, Commichau FM, Lewis RJ, Stülke J

    RNase Y in Bacillus subtilis: a Natively disordered protein that is the functional equivalent of RNase E from Escherichia coli

    J Bacteriol. 2011 Oct;193(19):5431-41. doi: 10.1128/JB.05500-11. Epub 2011 Jul 29. PubMed PMID: 21803996; PubMed Central PMCID: PMC3187381.
    Lehnik-Habrink M, Pförtner H, Rempeters L, Pietack N, Herzberg C, Stülke J

    The RNA degradosome in Bacillus subtilis: identification of CshA as the major RNA helicase in the multiprotein complex

    Mol Microbiol. 2010 Aug;77(4):958-71. doi: 10.1111/j.1365-2958.2010.07264.x. Epub 2010 Jun 21. PubMed PMID: 20572937.
    Muñoz-Márquez ME, Ponce-Rivas E

    Effect of pfkA chromosomal interruption on growth, sporulation, and production of organic acids in Bacillus subtilis

    J Basic Microbiol. 2010 Jun;50(3):232-40. doi: 10.1002/jobm.200900236. PubMed PMID: 20473954.
    Commichau FM, Rothe FM, Herzberg C, Wagner E, Hellwig D, Lehnik-Habrink M, Hammer E, Völker U, Stülke J

    Novel activities of glycolytic enzymes in Bacillus subtilis: interactions with essential proteins involved in mRNA processing

    Mol Cell Proteomics. 2009 Jun;8(6):1350-60. doi: 10.1074/mcp.M800546-MCP200. Epub 2009 Feb 3. PubMed PMID: 19193632; PubMed Central PMCID: PMC2690492.
    Meile JC, Wu LJ, Ehrlich SD, Errington J, Noirot P

    Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory

    Proteomics. 2006 Apr;6(7):2135-46. PubMed PMID: 16479537.
    Ludwig H, Homuth G, Schmalisch M, Dyka FM, Hecker M, Stülke J

    Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon

    Mol Microbiol. 2001 Jul;41(2):409-22. PubMed PMID: 11489127.
    Byrnes M, Zhu X, Younathan ES, Chang SH

    Kinetic characteristics of phosphofructokinase from Bacillus stearothermophilus: MgATP nonallosterically inhibits the enzyme

    Biochemistry. 1994 Mar 22;33(11):3424-31. PubMed PMID: 8136379.
    Zhu X, Byrnes M, Nelson JW, Chang SH

    Role of glycine 212 in the allosteric behavior of phosphofructokinase from Bacillus stearothermophilus

    Biochemistry. 1995 Feb 28;34(8):2560-5. PubMed PMID: 7873536.
    Marschke CK, Bernlohr RW

    Purification and characterization of phosphofructokinase of Bacillus licheniformis

    Arch Biochem Biophys. 1973 May;156(1):1-16. PubMed PMID: 4269800.
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