SubtiBank SubtiBank

It will happen very soon:

International Conference on Bacilli and Gram-positive bacteria

June 11 - 15, 2017, Berlin, Germany

Check out the program here


Categories containing this gene/protein

utilization of nucleotides, transcription factors and their control

This gene is a member of the following regulons

PucR regulon, TnrA regulon

The PucR regulon


  • Coordinates on the chromosome (coding sequence): 3,328,762 -> 3,330,357
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

  • PucR family
  • Paralogous protein(s)

    Kinetic information




    Effectors of protein activity




    Additional information

    Expression and Regulation


  • pucR-pucJ-pucK-pucL-pucM PubMed
  • Sigma factor


  • repressed by purine nucleotides (effector: allantoin) (PucR) PubMed
  • Regulatory mechanism

  • PucR: transcription auto-repression PubMed
  • TnrA: transcription activation PubMed
  • Additional information

    Biological materials


  • MGNA-A934 (yunI::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein


    Mirouze N, Bidnenko E, Noirot P, Auger S

    Genome-wide mapping of TnrA-binding sites provides new insights into the TnrA regulon in Bacillus subtilis

    Microbiologyopen. 2015 Jun;4(3):423-35. doi: 10.1002/mbo3.249. Epub 2015 Mar 8. PubMed PMID: 25755103; PubMed Central PMCID: PMC4475385.
    Yoshida K, Yamaguchi H, Kinehara M, Ohki YH, Nakaura Y, Fujita Y

    Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box

    Mol Microbiol. 2003 Jul;49(1):157-65. PubMed PMID: 12823818.
    Brandenburg JL, Wray LV Jr, Beier L, Jarmer H, Saxild HH, Fisher SH

    Roles of PucR, GlnR, and TnrA in regulating expression of the Bacillus subtilis ure P3 promoter

    J Bacteriol. 2002 Nov;184(21):6060-4. PubMed PMID: 12374841; PubMed Central PMCID: PMC135401.
    Beier L, Nygaard P, Jarmer H, Saxild HH

    Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism

    J Bacteriol. 2002 Jun;184(12):3232-41. PubMed PMID: 12029039; PubMed Central PMCID: PMC135105.
    Schultz AC, Nygaard P, Saxild HH

    Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator

    J Bacteriol. 2001 Jun;183(11):3293-302. PubMed PMID: 11344136; PubMed Central PMCID: PMC99626.
    No login