SubtiBank SubtiBank

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, transcription factors and their control

This gene is a member of the following regulons

PurR regulon

The PurR regulon


  • Coordinates on the chromosome (coding sequence): 54,441 -> 55,298
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

  • purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information




    Effectors of protein activity

  • PRPP acts as inducer, binding results in release of PurR from the operator PubMed
  • Structure

  • 1O57, 1O57 NCBI PubMed
  • Localization


  • PurR-Prs
  • Additional information

  • requires YabJ for in vivo activity
  • Expression and Regulation


  • purR-yabJ PubMed
  • Sigma factor


  • negative autoregulation, induction in the presence of PRPP PubMed
  • repressed in the presence of purine nucleotides (PurR) PubMed
  • Regulatory mechanism

  • repression by PurR PubMed
  • PurR: transcription repression PubMed
  • Additional information

    Biological materials


    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein



    Hove-Jensen B(1,)(2), Andersen KR(2), Kilstrup M(3), Martinussen J(3), Switzer RL(4), Willemoës M(5).

    Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.

    Microbiol Mol Biol Rev. 2016 Dec 28;81(1). pii: e00040-16. doi: 10.1128/MMBR.00040-16. Print 2017 Mar.DOI: 10.1128/MMBR.00040-16 PMCID: PMC5312242 [Available on 2017-12-28] PMID: 28031352

    Original publications

    Shi S, Chen T, Zhang Z, Chen X, Zhao X

    Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production

    Metab Eng. 2009 Jul-Sep;11(4-5):243-52. doi: 10.1016/j.ymben.2009.05.002. Epub 2009 May 13. PubMed PMID: 19446032.
    Nygaard P, Saxild HH

    The purine efflux pump PbuE in Bacillus subtilis modulates expression of the PurR and G-box (XptR) regulons by adjusting the purine base pool size

    J Bacteriol. 2005 Jan;187(2):791-4. PubMed PMID: 15629952; PubMed Central PMCID: PMC543539.
    Rappu P, Pullinen T, Mäntsälä P

    In vivo effect of mutations at the PRPP binding site of the Bacillus subtilis purine repressor

    J Bacteriol. 2003 Nov;185(22):6728-31. PubMed PMID: 14594850; PubMed Central PMCID: PMC262106.
    Bera AK, Zhu J, Zalkin H, Smith JL

    Functional dissection of the Bacillus subtilis pur operator site

    J Bacteriol. 2003 Jul;185(14):4099-109. PubMed PMID: 12837784; PubMed Central PMCID: PMC164870.
    Sinha SC, Krahn J, Shin BS, Tomchick DR, Zalkin H, Smith JL

    The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation

    J Bacteriol. 2003 Jul;185(14):4087-98. PubMed PMID: 12837783; PubMed Central PMCID: PMC164869.
    Saxild HH, Brunstedt K, Nielsen KI, Jarmer H, Nygaard P

    Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO

    J Bacteriol. 2001 Nov;183(21):6175-83. PubMed PMID: 11591660; PubMed Central PMCID: PMC100094.
    Weng M, Zalkin H

    Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo

    Curr Microbiol. 2000 Jul;41(1):56-9. PubMed PMID: 10919400.
    Rappu P, Shin BS, Zalkin H, Mäntsälä P

    A role for a highly conserved protein of unknown function in regulation of Bacillus subtilis purA by the purine repressor

    J Bacteriol. 1999 Jun;181(12):3810-5. PubMed PMID: 10368157; PubMed Central PMCID: PMC93860.
    Weng M, Nagy PL, Zalkin H

    Identification of the Bacillus subtilis pur operon repressor

    Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7455-9. PubMed PMID: 7638212; PubMed Central PMCID: PMC41358.
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