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Categories containing this gene/protein


transcription factors and their control, utilization of amino acids

This gene is a member of the following regulons


The PutR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 348,724 -> 349,959
  • Phenotypes of a mutant

  • no growth with proline as single source of carbon or nitrogen PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • transcription activation of the proline utilization operon putB-putC-putP in the presence of proline PubMed
  • Protein family

  • PucR family
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

  • proline acts as co-activator PubMed
  • Effectors of protein activity

  • proline activates PutR PubMed
  • Structure

    Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

  • putR PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • constitutively expressed PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Moses S, Sinner T, Zaprasis A, Stöveken N, Hoffmann T, Belitsky BR, Sonenshein AL, Bremer E

    Proline utilization by Bacillus subtilis: uptake and catabolism

    J Bacteriol. 2012 Feb;194(4):745-58. doi: 10.1128/JB.06380-11. Epub 2011 Dec 2. PubMed PMID: 22139509; PubMed Central PMCID: PMC3272947.
    Huang SC, Lin TH, Shaw GC

    PrcR, a PucR-type transcriptional activator, is essential for proline utilization and mediates proline-responsive expression of the proline utilization operon putBCP in Bacillus subtilis

    Microbiology. 2011 Dec;157(Pt 12):3370-7. doi: 10.1099/mic.0.054197-0. Epub 2011 Sep 29. PubMed PMID: 21964733.
    Belitsky BR

    Indirect repression by Bacillus subtilis CodY via displacement of the activator of the proline utilization operon

    J Mol Biol. 2011 Oct 21;413(2):321-36. doi: 10.1016/j.jmb.2011.08.003. Epub 2011 Aug 5. PubMed PMID: 21840319; PubMed Central PMCID: PMC3193587.
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