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June 11 - 15, 2017, Berlin, Germany

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Categories containing this gene/protein

transcription factors and their control, resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

This gene is a member of the following regulons

LmrA regulon, QdoR regulon

The QdoR regulon


  • Coordinates on the chromosome (coding sequence): 4,107,352 -> 4,107,927
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

  • TetR family
  • Paralogous protein(s)

  • LmrA
  • Kinetic information




    Effectors of protein activity

  • flavonoids such as quercetin act as molecular inducers and result in release from DNA targets PubMed
  • Structure

  • 1SGM
  • Localization


    Additional information

    Expression and Regulation


  • qdoR PubMed
  • Sigma factor


  • induced by flavonoids such as quercetin (QdoR, LmrA) PubMed
  • Regulatory mechanism

  • LmrA: transcription repression PubMed
  • QdoR: transcription repression PubMed
  • Additional information

    Biological materials


  • MGNA-B683 (yxaF::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein

  • Yasutaro Fujita, University of Fukuyama, Japan
  • References


    Hirooka K

    Transcriptional response machineries of Bacillus subtilis conducive to plant growth promotion

    Biosci Biotechnol Biochem. 2014;78(9):1471-84. doi: 10.1080/09168451.2014.943689. PubMed PMID: 25209494.
    Cuthbertson L, Nodwell JR

    The TetR family of regulators

    Microbiol Mol Biol Rev. 2013 Sep;77(3):440-75. doi: 10.1128/MMBR.00018-13. Review. PubMed PMID: 24006471; PubMed Central PMCID: PMC3811609.

    Original publications

    Hirooka K, Fujita Y

    Identification of aromatic residues critical to the DNA binding and ligand response of the Bacillus subtilis QdoR (YxaF) repressor antagonized by flavonoids

    Biosci Biotechnol Biochem. 2011;75(7):1325-34. Epub 2011 Jul 7. PubMed PMID: 21737930.
    Hirooka K, Kunikane S, Matsuoka H, Yoshida K, Kumamoto K, Tojo S, Fujita Y

    Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids

    J Bacteriol. 2007 Jul;189(14):5170-82. Epub 2007 May 4. PubMed PMID: 17483215; PubMed Central PMCID: PMC1951842.
    Yoshida K, Ishio I, Nagakawa E, Yamamoto Y, Yamamoto M, Fujita Y

    Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome

    Microbiology. 2000 Mar;146 ( Pt 3):573-9. PubMed PMID: 10746760.
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