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Categories containing this gene/protein

transcription factors and their control, biofilm formation, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The RemA regulon


  • Coordinates on the chromosome (coding sequence): 1,641,672 -> 1,641,941
  • The protein

    Catalyzed reaction/ biological activity

  • binds opuAA, tapA, and epsA promoter regions (upstream of the actual promoter) to activate transcription PubMed
  • binds DNA with high co-operativity due to low conserrvation of binding sites PubMed
  • Protein family

  • UPF0296 family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information




    Effectors of protein activity




    Additional information

    Expression and Regulation


    Sigma factor

  • SigF PubMed
  • Regulation

  • expressed during vegetative growth and early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein

  • Daniel Kearns, Indiana University, Bloomington, USA, homepage
  • References


    Cairns LS(1), Hobley L, Stanley-Wall NR.

    Biofilm formation by Bacillus subtilis: new insights into regulatory strategies and assembly mechanisms.

    Mol Microbiol. 2014 Aug;93(4):587-98. doi: 10.1111/mmi.12697. Epub 2014 Jul 18.DOI: 10.1111/mmi.12697 PMCID: PMC4238804 PMID: 24988880 [Indexed for MEDLINE]

    Original publications

    Takada H, Morita M, Shiwa Y, Sugimoto R, Suzuki S, Kawamura F, Yoshikawa H

    Cell motility and biofilm formation in Bacillus subtilis are affected by the ribosomal proteins, S11 and S21

    Biosci Biotechnol Biochem. 2014;78(5):898-907. doi: 10.1080/09168451.2014.915729. Epub 2014 May 28. PubMed PMID: 25035996.
    Winkelman JT, Bree AC, Bate AR, Eichenberger P, Gourse RL, Kearns DB

    RemA is a DNA-binding protein that activates biofilm matrix gene expression in Bacillus subtilis

    Mol Microbiol. 2013 Jun;88(5):984-97. doi: 10.1111/mmi.12235. Epub 2013 May 7. PubMed PMID: 23646920; PubMed Central PMCID: PMC3732408.
    Abecasis AB, Serrano M, Alves R, Quintais L, Pereira-Leal JB, Henriques AO

    A genomic signature and the identification of new sporulation genes

    J Bacteriol. 2013 May;195(9):2101-15. doi: 10.1128/JB.02110-12. Epub 2013 Feb 8. PubMed PMID: 23396918; PubMed Central PMCID: PMC3624599.
    Traag BA, Pugliese A, Eisen JA, Losick R

    Gene conservation among endospore-forming bacteria reveals additional sporulation genes in Bacillus subtilis

    J Bacteriol. 2013 Jan;195(2):253-60. doi: 10.1128/JB.01778-12. Epub 2012 Nov 2. PubMed PMID: 23123912; PubMed Central PMCID: PMC3553846.
    Cozy LM, Phillips AM, Calvo RA, Bate AR, Hsueh YH, Bonneau R, Eichenberger P, Kearns DB

    SlrA/SinR/SlrR inhibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of σ(D) in Bacillus subtilis

    Mol Microbiol. 2012 Mar;83(6):1210-28. doi: 10.1111/j.1365-2958.2012.08003.x. Epub 2012 Feb 22. PubMed PMID: 22329926; PubMed Central PMCID: PMC3303961.
    Winkelman JT, Blair KM, Kearns DB

    RemA (YlzA) and RemB (YaaB) regulate extracellular matrix operon expression and biofilm formation in Bacillus subtilis

    J Bacteriol. 2009 Jun;191(12):3981-91. doi: 10.1128/JB.00278-09. Epub 2009 Apr 10. PubMed PMID: 19363116; PubMed Central PMCID: PMC2698397.
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