SubtiBank SubtiBank

Description: two-component response regulator (OmpR family), regulation of aerobic and anaerobic respiration, activates expression of target genes in response to oxygen limitation

Name: resD
Locus: BSU23120BsubCyc
pI: 5.0
MW: 27.0 kDa
Protein length: 240 aaBLASTSequence
Gene length: 720 bpBLASTSequenceSequence + Flanks
Function: regulation of aerobic and anaerobic respiration
respiration
Product: two-component response regulator (OmpR family)
Essential: no
E.C. number:
Synonyms: ypxD
Databases: SubtiListKEGGUniProtExpression data browser

Categories containing this gene/protein


regulators of electron transport, transcription factors and their control, membrane proteins, phosphoproteins

This gene is a member of the following regulons


CcpA regulon, PhoP regulon, ResD regulon

The ResD regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,417,181 -> 2,417,903
  • Phenotypes of a mutant

  • a resD point mutation has been isolated upon selction at low pressure PubMed
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • OmpR family of two-component response regulators
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

  • phosphorylated by ResE on an Asp residue
  • Cofactors

    Effectors of protein activity

  • phosphorylation likely affects DNA-binding activity
  • Structure

    Localization

  • cell membrane (according to Swiss-Prot)
  • Interactions

  • ResD interacts with the RNA polymerase PubMed
  • ResE-ResD
  • Additional information

    Expression and Regulation


    Operon

    Sigma factor

  • resA: SigA PubMed
  • resD: SigA PubMed
  • Regulation

  • expressed under anaerobic conditions (ResD) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed
  • repressed by glucose (CcpA) PubMed
  • induced by daptomycin PubMed
  • Regulatory mechanism

  • ResD: transcription activation PubMed
  • PhoP: transcription activation PubMed
  • CcpA: transcription repression PubMed
  • Additional information

    Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Marion Hulett, University of Illinois at Chicago, USA Homepage
  • References


    Reviews

    Härtig E(1), Jahn D.

    Regulation of the anaerobic metabolism in Bacillus subtilis.

    Adv Microb Physiol. 2012;61:195-216. doi: 10.1016/B978-0-12-394423-8.00005-6.DOI: 10.1016/B978-0-12-394423-8.00005-6 PMID: 23046954 [Indexed for MEDLINE]

    Original publications

    Müller A, Wenzel M, Strahl H, Grein F, Saaki TN, Kohl B, Siersma T, Bandow JE, Sahl HG, Schneider T, Hamoen LW

    Daptomycin inhibits cell envelope synthesis by interfering with fluid membrane microdomains

    Proc Natl Acad Sci U S A. 2016 Oct 24. pii: 201611173. [Epub ahead of print] PubMed PMID: 27791134; PubMed Central PMCID: PMC5111643.
    Waters SM, Zeigler DR, Nicholson WL

    Experimental evolution of enhanced growth by Bacillus subtilis at low atmospheric pressure: genomic changes revealed by whole-genome sequencing

    Appl Environ Microbiol. 2015 Nov;81(21):7525-32. doi: 10.1128/AEM.01690-15. PubMed PMID: 26296725; PubMed Central PMCID: PMC4592849.
    Henares B, Kommineni S, Chumsakul O, Ogasawara N, Ishikawa S, Nakano MM

    The ResD response regulator, through functional interaction with NsrR and fur, plays three distinct roles in Bacillus subtilis transcriptional control

    J Bacteriol. 2014 Jan;196(2):493-503. doi: 10.1128/JB.01166-13. PubMed PMID: 24214949; PubMed Central PMCID: PMC3911253.
    Kommineni S, Lama A, Popescu B, Nakano MM

    Global transcriptional control by NsrR in Bacillus subtilis

    J Bacteriol. 2012 Apr;194(7):1679-88. doi: 10.1128/JB.06486-11. PubMed PMID: 22287527; PubMed Central PMCID: PMC3302466.
    Delumeau O, Lecointe F, Muntel J, Guillot A, Guédon E, Monnet V, Hecker M, Becher D, Polard P, Noirot P

    The dynamic protein partnership of RNA polymerase in Bacillus subtilis

    Proteomics. 2011 Aug;11(15):2992-3001. doi: 10.1002/pmic.201000790. PubMed PMID: 21710567.
    Kommineni S, Yukl E, Hayashi T, Delepine J, Geng H, Moënne-Loccoz P, Nakano MM

    Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter

    Mol Microbiol. 2010 Dec;78(5):1280-93. doi: 10.1111/j.1365-2958.2010.07407.x. PubMed PMID: 21091510; PubMed Central PMCID: PMC3075490.
    Puri-Taneja A, Schau M, Chen Y, Hulett FM

    Regulators of the Bacillus subtilis cydABCD operon: identification of a negative regulator, CcpA, and a positive regulator, ResD

    J Bacteriol. 2007 May;189(9):3348-58. PubMed PMID: 17322317; PubMed Central PMCID: PMC1855890.
    Nakano MM, Geng H, Nakano S, Kobayashi K

    The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression

    J Bacteriol. 2006 Aug;188(16):5878-87. PubMed PMID: 16885456; PubMed Central PMCID: PMC1540067.
    Härtig E, Hartmann A, Schätzle M, Albertini AM, Jahn D

    The Bacillus subtilis nrdEF genes, encoding a class Ib ribonucleotide reductase, are essential for aerobic and anaerobic growth

    Appl Environ Microbiol. 2006 Aug;72(8):5260-5. PubMed PMID: 16885274; PubMed Central PMCID: PMC1538738.
    Choi SK, Saier MH Jr

    Mechanism of CcpA-mediated glucose repression of the resABCDE operon of Bacillus subtilis

    J Mol Microbiol Biotechnol. 2006;11(1-2):104-10. PubMed PMID: 16825793.
    Schau M, Eldakak A, Hulett FM

    Terminal oxidases are essential to bypass the requirement for ResD for full Pho induction in Bacillus subtilis

    J Bacteriol. 2004 Dec;186(24):8424-32. PubMed PMID: 15576792; PubMed Central PMCID: PMC532433.
    Paul S, Zhang X, Hulett FM

    Two ResD-controlled promoters regulate ctaA expression in Bacillus subtilis

    J Bacteriol. 2001 May;183(10):3237-46. PubMed PMID: 11325953; PubMed Central PMCID: PMC95225.
    Nakano MM, Zhu Y

    Involvement of ResE phosphatase activity in down-regulation of ResD-controlled genes in Bacillus subtilis during aerobic growth

    J Bacteriol. 2001 Mar;183(6):1938-44. PubMed PMID: 11222591; PubMed Central PMCID: PMC95088.
    Zhang X, Hulett FM

    ResD signal transduction regulator of aerobic respiration in Bacillus subtilis: ctaA promoter regulation

    Mol Microbiol. 2000 Sep;37(5):1208-19. PubMed PMID: 10972837.
    Nakano MM, Zhu Y, Lacelle M, Zhang X, Hulett FM

    Interaction of ResD with regulatory regions of anaerobically induced genes in Bacillus subtilis

    Mol Microbiol. 2000 Sep;37(5):1198-207. PubMed PMID: 10972836.
    Fabret C, Feher VA, Hoch JA

    Two-component signal transduction in Bacillus subtilis: how one organism sees its world

    J Bacteriol. 1999 Apr;181(7):1975-83. Review. PubMed PMID: 10094672; PubMed Central PMCID: PMC93607.
    Birkey SM, Liu W, Zhang X, Duggan MF, Hulett FM

    Pho signal transduction network reveals direct transcriptional regulation of one two-component system by another two-component regulator: Bacillus subtilis PhoP directly regulates production of ResD

    Mol Microbiol. 1998 Dec;30(5):943-53. PubMed PMID: 9988472.
    Birkey SM, Liu W, Zhang X, Duggan MF, Hulett FM

    Pho signal transduction network reveals direct transcriptional regulation of one two-component system by another two-component regulator: Bacillus subtilis PhoP directly regulates production of ResD

    Mol Microbiol. 1998 Dec;30(5):943-53. PubMed PMID: 9988472.
    Nakano MM, Dailly YP, Zuber P, Clark DP

    Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth

    J Bacteriol. 1997 Nov;179(21):6749-55. PubMed PMID: 9352926; PubMed Central PMCID: PMC179605.
    Sun G, Sharkova E, Chesnut R, Birkey S, Duggan MF, Sorokin A, Pujic P, Ehrlich SD, Hulett FM

    Regulators of aerobic and anaerobic respiration in Bacillus subtilis

    J Bacteriol. 1996 Mar;178(5):1374-85. PubMed PMID: 8631715; PubMed Central PMCID: PMC177812.
    Sun G, Sharkova E, Chesnut R, Birkey S, Duggan MF, Sorokin A, Pujic P, Ehrlich SD, Hulett FM

    Regulators of aerobic and anaerobic respiration in Bacillus subtilis

    J Bacteriol. 1996 Mar;178(5):1374-85. PubMed PMID: 8631715; PubMed Central PMCID: PMC177812.
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