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Categories containing this gene/protein

regulators of electron transport, carbon core metabolism, transcription factors and their control, sporulation/ other

This gene is a member of the following regulons

The Rex regulon


  • Coordinates on the chromosome (coding sequence): 647,091 -> 647,738
  • Phenotypes of a mutant

  • inactivation of rex reduces sporulation efficiency to 9.2% that of wild type cells PubMed
  • inactivation of rex confers resistance to high concentrations of Zn(II) PubMed
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

  • transcriptional regulatory rex family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information




    Effectors of protein activity

  • NADH (indication of low oxygen concentration) releases Rex from DNA PubMed, Rex has a very highh affinity for NADH
  • Structure

  • 2VT2, 2VT3
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Interactions

    Additional information

    Expression and Regulation


  • ydiG-rex-tatAY-tatCY PubMed
  • Sigma factor


    Regulatory mechanism

    Additional information

    Biological materials


  • MGNA-C201 (ydiH::erm), available at the NBRP B. subtilis, Japan
  • LUW292 (erm), GP834 (erm), both available in the Stülke lab
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein

  • Claes von Wachenfeldt, Lund University, Sweden Homepage
  • References


    Härtig E(1), Jahn D.

    Regulation of the anaerobic metabolism in Bacillus subtilis.

    Adv Microb Physiol. 2012;61:195-216. doi: 10.1016/B978-0-12-394423-8.00005-6.DOI: 10.1016/B978-0-12-394423-8.00005-6 PMID: 23046954 [Indexed for MEDLINE]

    Original publications

    Chandrangsu P, Helmann JD

    Intracellular Zn(II) Intoxication Leads to Dysregulation of the PerR Regulon Resulting in Heme Toxicity in Bacillus subtilis

    PLoS Genet. 2016 Dec 9;12(12):e1006515. doi: 10.1371/journal.pgen.1006515. eCollection 2016 Dec. PubMed PMID: 27935957; PubMed Central PMCID: PMC5189952.
    Meeske AJ, Rodrigues CD, Brady J, Lim HC, Bernhardt TG, Rudner DZ

    High-Throughput Genetic Screens Identify a Large and Diverse Collection of New Sporulation Genes in Bacillus subtilis

    PLoS Biol. 2016 Jan 6;14(1):e1002341. doi: 10.1371/journal.pbio.1002341. eCollection 2016 Jan. PubMed PMID: 26735940; PubMed Central PMCID: PMC4703394.
    Laouami S, Clair G, Armengaud J, Duport C

    Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579

    PLoS One. 2014 Sep 12;9(9):e107354. doi: 10.1371/journal.pone.0107354. eCollection 2014. PubMed PMID: 25216269; PubMed Central PMCID: PMC4162614.
    Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P

    Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis

    Science. 2012 Mar 2;335(6072):1103-6. doi: 10.1126/science.1206848. PubMed PMID: 22383849.
    Wang E, Ikonen TP, Knaapila M, Svergun D, Logan DT, von Wachenfeldt C

    Small-angle X-ray scattering study of a Rex family repressor: conformational response to NADH and NAD+ binding in solution

    J Mol Biol. 2011 May 13;408(4):670-83. doi: 10.1016/j.jmb.2011.02.050. Epub 2011 Mar 21. PubMed PMID: 21402078.
    Irnov I, Sharma CM, Vogel J, Winkler WC

    Identification of regulatory RNAs in Bacillus subtilis

    Nucleic Acids Res. 2010 Oct;38(19):6637-51. doi: 10.1093/nar/gkq454. Epub 2010 Jun 4. PubMed PMID: 20525796; PubMed Central PMCID: PMC2965217.
    Wang E, Bauer MC, Rogstam A, Linse S, Logan DT, von Wachenfeldt C

    Structure and functional properties of the Bacillus subtilis transcriptional repressor Rex

    Mol Microbiol. 2008 Jul;69(2):466-78. doi: 10.1111/j.1365-2958.2008.06295.x. PubMed PMID: 18485070.
    Gyan S, Shiohira Y, Sato I, Takeuchi M, Sato T

    Regulatory loop between redox sensing of the NADH/NAD(+) ratio by Rex (YdiH) and oxidation of NADH by NADH dehydrogenase Ndh in Bacillus subtilis

    J Bacteriol. 2006 Oct;188(20):7062-71. PubMed PMID: 17015645; PubMed Central PMCID: PMC1636230.
    Larsson JT, Rogstam A, von Wachenfeldt C

    Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis

    Microbiology. 2005 Oct;151(Pt 10):3323-35. PubMed PMID: 16207915.
    Schau M, Chen Y, Hulett FM

    Bacillus subtilis YdiH is a direct negative regulator of the cydABCD operon

    J Bacteriol. 2004 Jul;186(14):4585-95. PubMed PMID: 15231791; PubMed Central PMCID: PMC438603.
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