SubtiBank SubtiBank

Categories containing this gene/protein

RNAses, translation, essential genes

This gene is a member of the following regulons


  • Coordinates on the chromosome (coding sequence): 4,214,753 -> 4,215,103
  • Phenotypes of a mutant

  • essential PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor (according to Swiss-Prot)
  • 5' end maturation of tmRNA PubMed
  • Protein family

  • rnpA family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information


  • RNR motif: residues 60-68, stabilizes complexes with both the RNA part of RNase P (rnpB) and with pre-tRNA (rnpB-RnpA-pre-tRNA) PubMed
  • Modification


    Effectors of protein activity


  • 1A6F PubMed
  • 3Q1R (complex of RnpA with the RNA RnpB and tRNA, from Thermotoga maritima) PubMed
  • Localization


  • forms dimers PubMed
  • RnpA-RpmH
  • RnpA-RnpB PubMed
  • RnpA-CshA PubMed
  • Additional information

    Expression and Regulation


    Sigma factor


  • expression transiently increases in the forespore PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein

  • Carol Fierke, University of Michigan, Ann Arbor, USA homepage
  • Roland Hartmann, Marburg University, Germany homepage
  • References


    Hartmann RK, Gössringer M, Späth B, Fischer S, Marchfelder A

    The making of tRNAs and more - RNase P and tRNase Z

    Prog Mol Biol Transl Sci. 2009;85:319-68. doi: 10.1016/S0079-6603(08)00808-8. Review. PubMed PMID: 19215776.
    Smith JK, Hsieh J, Fierke CA

    Importance of RNA-protein interactions in bacterial ribonuclease P structure and catalysis

    Biopolymers. 2007 Dec 5-15;87(5-6):329-38. Review. PubMed PMID: 17868095.
    Kazantsev AV, Pace NR

    Bacterial RNase P: a new view of an ancient enzyme

    Nat Rev Microbiol. 2006 Oct;4(10):729-40. Review. PubMed PMID: 16980936.
    Evans D, Marquez SM, Pace NR

    RNase P: interface of the RNA and protein worlds

    Trends Biochem Sci. 2006 Jun;31(6):333-41. Epub 2006 May 6. Review. PubMed PMID: 16679018.
    Torres-Larios A, Swinger KK, Pan T, Mondragón A

    Structure of ribonuclease P--a universal ribozyme

    Curr Opin Struct Biol. 2006 Jun;16(3):327-35. Epub 2006 May 2. Review. PubMed PMID: 16650980.
    Hsieh J, Andrews AJ, Fierke CA

    Roles of protein subunits in RNA-protein complexes: lessons from ribonuclease P

    Biopolymers. 2004 Jan;73(1):79-89. Review. PubMed PMID: 14691942.
    Hartmann E, Hartmann RK

    The enigma of ribonuclease P evolution

    Trends Genet. 2003 Oct;19(10):561-9. Review. PubMed PMID: 14550630.
    Kirsebom LA, Vioque A

    RNase P from bacteria

    Substrate recognition and function of the protein subunit. Mol Biol Rep. 1995-1996;22(2-3):99-109. Review. PubMed PMID: 8901495.

    Original Publications

    Chen Y, Liu X, Wu N, Fierke CA

    Fluorescence-Based Real-Time Activity Assays to Identify RNase P Inhibitors

    Methods Mol Biol. 2017;1520:201-225. PubMed PMID: 27873254.
    Mosley PL, Daniels KG, Oas TG

    Electrostatic Energetics of Bacillus subtilis Ribonuclease P Protein Determined by Nuclear Magnetic Resonance-Based Histidine pKa Measurements

    Biochemistry. 2015 Sep 8;54(35):5379-88. doi: 10.1021/acs.biochem.5b00138. Epub 2015 Aug 20. PubMed PMID: 26267651; PubMed Central PMCID: PMC4696772.
    Gilet L, DiChiara JM, Figaro S, Bechhofer DH, Condon C

    Small stable RNA maturation and turnover in Bacillus subtilis

    Mol Microbiol. 2015 Jan;95(2):270-82. doi: 10.1111/mmi.12863. Epub 2014 Dec 19. PubMed PMID: 25402410; PubMed Central PMCID: PMC4364036.
    Liu X, Chen Y, Fierke CA

    A real-time fluorescence polarization activity assay to screen for inhibitors of bacterial ribonuclease P

    Nucleic Acids Res. 2014 Nov 10;42(20):e159. doi: 10.1093/nar/gku850. Epub 2014 Sep 23. PubMed PMID: 25249623; PubMed Central PMCID: PMC4227764.
    Daniels KG, Tonthat NK, McClure DR, Chang YC, Liu X, Schumacher MA, Fierke CA, Schmidler SC, Oas TG

    Ligand concentration regulates the pathways of coupled protein folding and binding

    J Am Chem Soc. 2014 Jan 22;136(3):822-5. doi: 10.1021/ja4086726. Epub 2014 Jan 9. PubMed PMID: 24364358; PubMed Central PMCID: PMC3977005.
    Rosenberg A, Sinai L, Smith Y, Ben-Yehuda S

    Dynamic expression of the translational machinery during Bacillus subtilis life cycle at a single cell level

    PLoS One. 2012;7(7):e41921. doi: 10.1371/journal.pone.0041921. Epub 2012 Jul 25. PubMed PMID: 22848659; PubMed Central PMCID: PMC3405057.
    Roux CM, DeMuth JP, Dunman PM

    Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex

    J Bacteriol. 2011 Oct;193(19):5520-6. doi: 10.1128/JB.05485-11. Epub 2011 Jul 15. PubMed PMID: 21764917; PubMed Central PMCID: PMC3187390.
    Koutmou KS, Day-Storms JJ, Fierke CA

    The RNR motif of B

    subtilis RNase P protein interacts with both PRNA and pre-tRNA to stabilize an active conformer. RNA. 2011 Jul;17(7):1225-35. doi: 10.1261/rna.2742511. Epub 2011 May 27. PubMed PMID: 21622899; PubMed Central PMCID: PMC3138560.
    Reiter NJ, Osterman A, Torres-Larios A, Swinger KK, Pan T, Mondragón A

    Structure of a bacterial ribonuclease P holoenzyme in complex with tRNA

    Nature. 2010 Dec 9;468(7325):784-9. doi: 10.1038/nature09516. Epub 2010 Nov 14. PubMed PMID: 21076397; PubMed Central PMCID: PMC3058908.
    Chang YC, Franch WR, Oas TG

    Probing the folding intermediate of Bacillus subtilis RNase P protein by nuclear magnetic resonance

    Biochemistry. 2010 Nov 9;49(44):9428-37. doi: 10.1021/bi100287y. PubMed PMID: 20843005; PubMed Central PMCID: PMC3081390.
    Chang YC, Oas TG

    Osmolyte-induced folding of an intrinsically disordered protein: folding mechanism in the absence of ligand

    Biochemistry. 2010 Jun 29;49(25):5086-96. doi: 10.1021/bi100222h. PubMed PMID: 20476778; PubMed Central PMCID: PMC2937257.
    Koutmou KS, Zahler NH, Kurz JC, Campbell FE, Harris ME, Fierke CA

    Protein-precursor tRNA contact leads to sequence-specific recognition of 5' leaders by bacterial ribonuclease P

    J Mol Biol. 2010 Feb 12;396(1):195-208. doi: 10.1016/j.jmb.2009.11.039. Epub 2009 Nov 28. PubMed PMID: 19932118; PubMed Central PMCID: PMC2829246.
    Hsieh J, Fierke CA

    Conformational change in the Bacillus subtilis RNase P holoenzyme--pre-tRNA complex enhances substrate affinity and limits cleavage rate

    RNA. 2009 Aug;15(8):1565-77. doi: 10.1261/rna.1639409. Epub 2009 Jun 23. PubMed PMID: 19549719; PubMed Central PMCID: PMC2714742.
    Gösringer M, Hartmann RK

    Function of heterologous and truncated RNase P proteins in Bacillus subtilis

    Mol Microbiol. 2007 Nov;66(3):801-13. Epub 2007 Oct 5. PubMed PMID: 17919279.
    Wegscheid B, Hartmann RK

    In vivo and in vitro investigation of bacterial type B RNase P interaction with tRNA 3'-CCA

    Nucleic Acids Res. 2007;35(6):2060-73. Epub 2007 Mar 13. PubMed PMID: 17355991; PubMed Central PMCID: PMC1874595.
    Niranjanakumari S, Day-Storms JJ, Ahmed M, Hsieh J, Zahler NH, Venters RA, Fierke CA

    Probing the architecture of the B

    subtilis RNase P holoenzyme active site by cross-linking and affinity cleavage. RNA. 2007 Apr;13(4):521-35. Epub 2007 Feb 13. PubMed PMID: 17299131; PubMed Central PMCID: PMC1831860.
    Gössringer M, Kretschmer-Kazemi Far R, Hartmann RK

    Analysis of RNase P protein (rnpA) expression in Bacillus subtilis utilizing strains with suppressible rnpA expression

    J Bacteriol. 2006 Oct;188(19):6816-23. PubMed PMID: 16980484; PubMed Central PMCID: PMC1595511.
    Henkels CH, Oas TG

    Thermodynamic characterization of the osmolyte- and ligand-folded states of Bacillus subtilis ribonuclease P protein

    Biochemistry. 2005 Oct 4;44(39):13014-26. PubMed PMID: 16185070.
    Rox C, Feltens R, Pfeiffer T, Hartmann RK

    Potential contact sites between the protein and RNA subunit in the Bacillus subtilis RNase P holoenzyme

    J Mol Biol. 2002 Jan 25;315(4):551-60. PubMed PMID: 11812129.
    Hansen A, Pfeiffer T, Zuleeg T, Limmer S, Ciesiolka J, Feltens R, Hartmann RK

    Exploring the minimal substrate requirements for trans-cleavage by RNase P holoenzymes from Escherichia coli and Bacillus subtilis

    Mol Microbiol. 2001 Jul;41(1):131-43. PubMed PMID: 11454206.
    Henkels CH, Kurz JC, Fierke CA, Oas TG

    Linked folding and anion binding of the Bacillus subtilis ribonuclease P protein

    Biochemistry. 2001 Mar 6;40(9):2777-89. PubMed PMID: 11258888.
    Warnecke JM, Held R, Busch S, Hartmann RK

    Role of metal ions in the hydrolysis reaction catalyzed by RNase P RNA from Bacillus subtilis

    J Mol Biol. 1999 Jul 9;290(2):433-45. PubMed PMID: 10390342.
    Stams T, Niranjanakumari S, Fierke CA, Christianson DW

    Ribonuclease P protein structure: evolutionary origins in the translational apparatus

    Science. 1998 May 1;280(5364):752-5. PubMed PMID: 9563955.
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