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Categories containing this gene/protein


utilization of amino acids, transcription factors and their control, glutamate metabolism, Transcription factors activating transcription at SigL-dependent promoters

This gene is a member of the following regulons


The RocR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 4,145,747 -> 4,147,132
  • The protein


    Catalyzed reaction/ biological activity

  • transcription activation at SigL-dependent promoters of rocABC, rocDEF, and rocG
  • Protein family

  • Sigma-54 interacting transcription activator
  • Transcription factors activating transcription at SigL-dependent promoters
  • Paralogous protein(s)

    Kinetic information

    Domains

  • Sigma-54 factor interaction domain (143–372)
  • 2x nucleotid-binding domain (171–178),(233–242)
  • HTH motif (434–453)
  • PAS domain
  • Modification

    Cofactors

  • ornithine or citrulline are required for RocR-dependent transcription activation PubMed
  • Effectors of protein activity

    Structure

    Localization

    Interactions

  • RocR-SigL
  • Additional information

    Expression and Regulation


    Operon

  • rocR (according to DBTBS)
  • Sigma factor

  • SigA PubMed
  • Regulation

  • autoregulation by RocR PubMed
  • Regulatory mechanism

  • autorepression
  • Additional information

    Biological materials


    Mutant

  • GP2312 (rocR::tet, available in Jörg Stülke's lab)
  • QB5533 (aphA3), from Michel Debarbouille, available in Jörg Stülke's lab
  • 1A911 ( rocR::kan), PubMed, available at BGSC
  • BKE40350 (rocR::erm, available in the BGSC, in Fabian Commichaus, and in Jörg Stülkes lab)
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Michel Debarbouille, Pasteur Institute, Paris, France Homepage
  • References


    Zaprasis A, Hoffmann T, Wünsche G, Flórez LA, Stülke J, Bremer E

    Mutational activation of the RocR activator and of a cryptic rocDEF promoter bypass loss of the initial steps of proline biosynthesis in Bacillus subtilis

    Environ Microbiol. 2014 Mar;16(3):701-17. doi: 10.1111/1462-2920.12193. Epub 2013 Jul 19. PubMed PMID: 23869754.
    Manabe K, Kageyama Y, Morimoto T, Ozawa T, Sawada K, Endo K, Tohata M, Ara K, Ozaki K, Ogasawara N

    Combined effect of improved cell yield and increased specific productivity enhances recombinant enzyme production in genome-reduced Bacillus subtilis strain MGB874

    Appl Environ Microbiol. 2011 Dec;77(23):8370-81. doi: 10.1128/AEM.06136-11. Epub 2011 Sep 30. PubMed PMID: 21965396; PubMed Central PMCID: PMC3233064.
    Joly N, Zhang N, Buck M, Zhang X

    Coupling AAA protein function to regulated gene expression

    Biochim Biophys Acta. 2012 Jan;1823(1):108-16. doi: 10.1016/j.bbamcr.2011.08.012. Epub 2011 Aug 31. Review. PubMed PMID: 21906631.
    Ali NO, Jeusset J, Larquet E, Le Cam E, Belitsky B, Sonenshein AL, Msadek T, Débarbouillé M

    Specificity of the interaction of RocR with the rocG-rocA intergenic region in Bacillus subtilis

    Microbiology. 2003 Mar;149(Pt 3):739-50. PubMed PMID: 12634342.
    Gardan R, Rapoport G, Débarbouillé M

    Role of the transcriptional activator RocR in the arginine-degradation pathway of Bacillus subtilis

    Mol Microbiol. 1997 May;24(4):825-37. PubMed PMID: 9194709.
    Calogero S, Gardan R, Glaser P, Schweizer J, Rapoport G, Debarbouille M

    RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators

    J Bacteriol. 1994 Mar;176(5):1234-41. PubMed PMID: 8113162; PubMed Central PMCID: PMC205184.
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