SubtiBank SubtiBank

Categories containing this gene/protein


translation, RNA binding regulators, essential genes, membrane proteins

This gene is a member of the following regulons


RplT regulon, stringent response

The RplT regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,952,224 -> 2,952,583
  • Phenotypes of a mutant

  • essential PubMed
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • ribosomal protein L20P family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 3J9W (the ribosome) PubMed
  • Localization

  • cell membrane (according to Swiss-Prot)
  • Interactions

    Additional information

    Expression and Regulation


    Operon

  • infC-rpmI-rplT-ysdA PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism

  • binding of RplT to a RNA switch in the infC leader region causes transcription termination PubMed
  • Additional information

  • expression of IF3 is uncoupled from that of L35 and L20 by RNase Y-dependent processing PubMed
  • Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Sohmen D, Chiba S, Shimokawa-Chiba N, Innis CA, Berninghausen O, Beckmann R, Ito K, Wilson DN

    Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling

    Nat Commun. 2015 Apr 23;6:6941. doi: 10.1038/ncomms7941. PubMed PMID: 25903689; PubMed Central PMCID: PMC4423224.
    Deiorio-Haggar K, Anthony J, Meyer MM

    RNA structures regulating ribosomal protein biosynthesis in bacilli

    RNA Biol. 2013 Jul;10(7):1180-4. doi: 10.4161/rna.24151. PubMed PMID: 23611891; PubMed Central PMCID: PMC3849166.
    Akanuma G, Nanamiya H, Natori Y, Yano K, Suzuki S, Omata S, Ishizuka M, Sekine Y, Kawamura F

    Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation

    J Bacteriol. 2012 Nov;194(22):6282-91. doi: 10.1128/JB.01544-12. PubMed PMID: 23002217; PubMed Central PMCID: PMC3486396.
    Bruscella P, Shahbabian K, Laalami S, Putzer H

    RNase Y is responsible for uncoupling the expression of translation factor IF3 from that of the ribosomal proteins L35 and L20 in Bacillus subtilis

    Mol Microbiol. 2011 Sep;81(6):1526-41. doi: 10.1111/j.1365-2958.2011.07793.x. PubMed PMID: 21843271.
    Lauber MA, Running WE, Reilly JP

    B

    subtilis ribosomal proteins: structural homology and post-translational modifications. J Proteome Res. 2009 Sep;8(9):4193-206. doi: 10.1021/pr801114k. PubMed PMID: 19653700.
    Choonee N, Even S, Zig L, Putzer H

    Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism

    Nucleic Acids Res. 2007;35(5):1578-88. PubMed PMID: 17289755; PubMed Central PMCID: PMC1865079.
    Eymann C, Homuth G, Scharf C, Hecker M

    Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis

    J Bacteriol. 2002 May;184(9):2500-20. PubMed PMID: 11948165; PubMed Central PMCID: PMC134987.
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