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Categories containing this gene/protein


sigma factors and their control, sporulation proteins

This gene is a member of the following regulons


RsfA regulon, SigF regulon, SigG regulon

The RsfA regulon


Gene


  • Coordinates on the chromosome (coding sequence): 3,861,437 -> 3,862,213
  • Phenotypes of a mutant

  • inactivation of rsfA reduces sporulation efficiency to 11.6% that of wild type cells PubMed
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

    Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

  • forespore (according to Swiss-Prot)
  • Interactions

    Additional information

    Expression and Regulation


    Operon

  • rsfA PubMed
  • Sigma factor

  • SigF PubMed, SigG PubMed
  • Regulation

  • expressed during sporulation in the forespore (SigF) PubMed
  • repressed by casamino acids PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • MGNA-A592 (ywfN::erm), available at the NBRP B. subtilis, Japan
  • 1S132 ( rsfA::tet), PubMed, available at BGSC
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Meeske AJ, Rodrigues CD, Brady J, Lim HC, Bernhardt TG, Rudner DZ

    High-Throughput Genetic Screens Identify a Large and Diverse Collection of New Sporulation Genes in Bacillus subtilis

    PLoS Biol. 2016 Jan 6;14(1):e1002341. doi: 10.1371/journal.pbio.1002341. eCollection 2016 Jan. PubMed PMID: 26735940; PubMed Central PMCID: PMC4703394.
    Wang ST, Setlow B, Conlon EM, Lyon JL, Imamura D, Sato T, Setlow P, Losick R, Eichenberger P

    The forespore line of gene expression in Bacillus subtilis

    J Mol Biol. 2006 Apr 21;358(1):16-37. Epub 2006 Feb 8. PubMed PMID: 16497325.
    Steil L, Serrano M, Henriques AO, Völker U

    Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis

    Microbiology. 2005 Feb;151(Pt 2):399-420. PubMed PMID: 15699190.
    Mäder U, Homuth G, Scharf C, Büttner K, Bode R, Hecker M

    Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability

    J Bacteriol. 2002 Aug;184(15):4288-95. PubMed PMID: 12107147; PubMed Central PMCID: PMC135197.
    Wu LJ, Errington J

    Identification and characterization of a new prespore-specific regulatory gene, rsfA, of Bacillus subtilis

    J Bacteriol. 2000 Jan;182(2):418-24. PubMed PMID: 10629188; PubMed Central PMCID: PMC94291.
    Glaser P, Kunst F, Arnaud M, Coudart MP, Gonzales W, Hullo MF, Ionescu M, Lubochinsky B, Marcelino L, Moszer I, et al

    Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees

    Mol Microbiol. 1993 Oct;10(2):371-84. PubMed PMID: 7934828.
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