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Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins

This gene is a member of the following regulons

SacY regulon

The SacY regulon


  • Coordinates on the chromosome (coding sequence): 3,943,667 -> 3,944,509
  • The protein

    Catalyzed reaction/ biological activity

  • binding to the mRNA of sacB and the sacX-sacY operon, causes transcription antitermination (in presence of sucrose)
  • Protein family

  • [PRD-containing transcription factors] bglG family (according to Swiss-Prot) BglG family of antiterminators
  • Paralogous protein(s)

  • LicT, GlcT, SacT
  • Kinetic information


  • N-terminal RNA binding domain PubMed
  • 2 x PRD (PTS regulation domains) PubMed
  • Modification


    Effectors of protein activity


  • 1AUU (RNA-binding domain)
  • Localization


  • PtsH-SacY
  • Additional information

    Expression and Regulation


  • sacX-sacY PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • induction by sucrose (at high concentration) PubMed
  • Regulatory mechanism

  • induction by SacY-dependent RNA switch (transcriptional antitermination) PubMed
  • DegU-P: transcription activation PubMed
  • Additional information

    Biological materials


  • GP425 (cat), available in Jörg Stülke's lab
  • Expression vector

  • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag, in pWH844: pGP316, available in Jörg Stülke's lab
  • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP573, available in Jörg Stülke's lab
  • lacZ fusion

    GFP fusion

  • GP1226 (spc, based on pGP1870), available in Jörg Stülke's lab
  • two-hybrid system

    FLAG-tag construct

  • GP1222 (spc, based on pGP1331), available in Jörg Stülke's lab
  • Antibody

    Labs working on this gene/protein

  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
  • References


    Commichau FM, Stülke J

    Trigger enzymes: bifunctional proteins active in metabolism and in controlling gene expression

    Mol Microbiol. 2008 Feb;67(4):692-702. Epub 2007 Dec 11. Review. PubMed PMID: 18086213.
    Stülke J, Arnaud M, Rapoport G, Martin-Verstraete I

    PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria

    Mol Microbiol. 1998 Jun;28(5):865-74. Review. PubMed PMID: 9663674.

    Original publications

    Clerte C, Declerck N, Margeat E

    Competitive folding of anti-terminator/terminator hairpins monitored by single molecule FRET

    Nucleic Acids Res. 2013 Feb 1;41(4):2632-43. doi: 10.1093/nar/gks1315. Epub 2013 Jan 8. PubMed PMID: 23303779; PubMed Central PMCID: PMC3575810.
    Hübner S, Declerck N, Diethmaier C, Le Coq D, Aymerich S, Stülke J

    Prevention of cross-talk in conserved regulatory systems: identification of specificity determinants in RNA-binding anti-termination proteins of the BglG family

    Nucleic Acids Res. 2011 May;39(10):4360-72. doi: 10.1093/nar/gkr021. Epub 2011 Jan 28. PubMed PMID: 21278164; PubMed Central PMCID: PMC3105407.
    Declerck N, Minh NL, Yang Y, Bloch V, Kochoyan M, Aymerich S

    RNA recognition by transcriptional antiterminators of the BglG/SacY family: mapping of SacY RNA binding site

    J Mol Biol. 2002 Jun 21;319(5):1035-48. PubMed PMID: 12079345.
    Tortosa P, Declerck N, Dutartre H, Lindner C, Deutscher J, Le Coq D

    Sites of positive and negative regulation in the Bacillus subtilis antiterminators LicT and SacY

    Mol Microbiol. 2001 Sep;41(6):1381-93. PubMed PMID: 11580842.
    Declerck N, Vincent F, Hoh F, Aymerich S, van Tilbeurgh H

    RNA recognition by transcriptional antiterminators of the BglG/SacY family: functional and structural comparison of the CAT domain from SacY and LicT

    J Mol Biol. 1999 Nov 26;294(2):389-402. PubMed PMID: 10610766.
    van Tilbeurgh H, Manival X, Aymerich S, Lhoste JM, Dumas C, Kochoyan M

    Crystal structure of a new RNA-binding domain from the antiterminator protein SacY of Bacillus subtilis

    EMBO J. 1997 Aug 15;16(16):5030-6. PubMed PMID: 9305644; PubMed Central PMCID: PMC1170137.
    Manival X, Yang Y, Strub MP, Kochoyan M, Steinmetz M, Aymerich S

    From genetic to structural characterization of a new class of RNA-binding domain within the SacY/BglG family of antiterminator proteins

    EMBO J. 1997 Aug 15;16(16):5019-29. PubMed PMID: 9305643; PubMed Central PMCID: PMC1170136.
    Tortosa P, Aymerich S, Lindner C, Saier MH Jr, Reizer J, Le Coq D

    Multiple phosphorylation of SacY, a Bacillus subtilis transcriptional antiterminator negatively controlled by the phosphotransferase system

    J Biol Chem. 1997 Jul 4;272(27):17230-7. PubMed PMID: 9202047.
    Arnaud M, Débarbouillé M, Rapoport G, Saier MH Jr, Reizer J

    In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis

    J Biol Chem. 1996 Aug 2;271(31):18966-72. PubMed PMID: 8702561.
    Tortosa P, Le Coq D

    A ribonucleic antiterminator sequence (RAT) and a distant palindrome are both involved in sucrose induction of the Bacillus subtilis sacXY regulatory operon

    Microbiology. 1995 Nov;141 ( Pt 11):2921-7. PubMed PMID: 8535520.
    Zukowski MM, Miller L, Cosgwell P, Chen K, Aymerich S, Steinmetz M

    Nucleotide sequence of the sacS locus of Bacillus subtilis reveals the presence of two regulatory genes

    Gene. 1990 May 31;90(1):153-5. PubMed PMID: 2116367.
    Crutz AM, Steinmetz M, Aymerich S, Richter R, Le Coq D

    Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system

    J Bacteriol. 1990 Feb;172(2):1043-50. PubMed PMID: 2105292; PubMed Central PMCID: PMC208535.
    Crutz AM, Steinmetz M

    Transcription of the Bacillus subtilis sacX and sacY genes, encoding regulators of sucrose metabolism, is both inducible by sucrose and controlled by the DegS-DegU signalling system

    J Bacteriol. 1992 Oct;174(19):6087-95. PubMed PMID: 1400159; PubMed Central PMCID: PMC207674.
    Aymerich S, Steinmetz M

    Specificity determinants and structural features in the RNA target of the bacterial antiterminator proteins of the BglG/SacY family

    Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10410-4. PubMed PMID: 1279678; PubMed Central PMCID: PMC50348.
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