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Categories containing this gene/protein


transcription factors and their control, transition state regulators

This gene is a member of the following regulons


SalA regulon

The SalA regulon


Gene


  • Coordinates on the chromosome (coding sequence): 157,421 -> 158,479
  • Phenotypes of a mutant

  • increased expression of aprE (due to loss of repression of scoC expression)
  • The protein


    Catalyzed reaction/ biological activity

  • transcription repression of scoC expression 26094643
  • Protein family

  • Mrp/NBP35 ATP-binding proteins family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

  • DNA-binding domain: aa 1 - 60 PubMed
  • ATP-binding Walker domain: aa 105 - 275 PubMed
  • domain for the interaction with PtkA: aa 294 - 352
  • Modification

  • PtkA-dependent phosphorylation at Tyr-327 results in better binding of the target promoter PubMed
  • Cofactors

    Effectors of protein activity

    Structure

    Localization

    Interactions

  • SalA-PtkA, for phosphorylation of SalA PubMed, to activate PtkA activity PubMed
  • Additional information

    Expression and Regulation


    Operon

    Sigma factor

    Regulation

  • negative autoregulation
  • Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • 1A919 ( salA::erm), PubMed, available at BGSC
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Derouiche A, Shi L, Kalantari A, Mijakovic I

    Substrate Specificity of the Bacillus subtilis BY-Kinase PtkA Is Controlled by Alternative Activators: TkmA and SalA

    Front Microbiol. 2016 Sep 26;7:1525. eCollection 2016. PubMed PMID: 27725816; PubMed Central PMCID: PMC5035731.
    Derouiche A, Shi L, Kalantari A, Mijakovic I

    Evolution and tinkering: what do a protein kinase, a transcriptional regulator and chromosome segregation/cell division proteins have in common

    Curr Genet. 2016 Feb;62(1):67-70. doi: 10.1007/s00294-015-0513-y. Epub 2015 Aug 19. Review. PubMed PMID: 26286503.
    Derouiche A, Shi L, Bidnenko V, Ventroux M, Pigonneau N, Franz-Wachtel M, Kalantari A, Nessler S, Noirot-Gros MF, Mijakovic I

    Bacillus subtilis SalA is a phosphorylation-dependent transcription regulator that represses scoC and activates the production of the exoprotease AprE

    Mol Microbiol. 2015 Sep;97(6):1195-208. doi: 10.1111/mmi.13098. Epub 2015 Jul 17. PubMed PMID: 26094643.
    Ogura M, Matsuzawa A, Yoshikawa H, Tanaka T

    Bacillus subtilis SalA (YbaL) negatively regulates expression of scoC, which encodes the repressor for the alkaline exoprotease gene, aprE

    J Bacteriol. 2004 May;186(10):3056-64. PubMed PMID: 15126467; PubMed Central PMCID: PMC400612.
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