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Categories containing this gene/protein

transcription, sigma factors and their control, heat shock proteins

This gene is a member of the following regulons

SigI regulon, WalR regulon

The SigI regulon


  • Coordinates on the chromosome (coding sequence): 1,411,892 -> 1,412,647
  • Phenotypes of a mutant

  • temperature-sensitive growth on agar plates PubMed
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

    Paralogous protein(s)

    Kinetic information




    Effectors of protein activity




  • RsgI-SigI, the interaction inhibits SigI activity PubMed
  • SigI-RpoB-RpoC
  • Additional information

  • overexpression of SigI suppresses mreB mreBH mbl mutants PubMed
  • Expression and Regulation


  • sigI-rsgI PubMed
  • Sigma factor

  • SigI PubMed
  • Regulation

  • induced at high temperature (SigI, WalR) PubMed
  • Regulatory mechanism

  • WalR: transcription activation PubMed
  • Additional information

    Biological materials


  • MGNA-A229 (ykoZ::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein


    Kim JG, Wilson AC

    Loss of σI affects heat-shock response and virulence gene expression in Bacillus anthracis

    Microbiology. 2016 Mar;162(3):564-74. doi: 10.1099/mic.0.000236. Epub 2016 Jan 6. PubMed PMID: 26744224.
    Huang WZ, Wang JJ, Chen HJ, Chen JT, Shaw GC

    The heat-inducible essential response regulator WalR positively regulates transcription of sigI, mreBH and lytE in Bacillus subtilis under heat stress

    Res Microbiol. 2013 Dec;164(10):998-1008. doi: 10.1016/j.resmic.2013.10.003. Epub 2013 Oct 11. PubMed PMID: 24125693.
    Salzberg LI, Powell L, Hokamp K, Botella E, Noone D, Devine KM

    The WalRK (YycFG) and σ(I) RsgI regulators cooperate to control CwlO and LytE expression in exponentially growing and stressed Bacillus subtilis cells

    Mol Microbiol. 2013 Jan;87(1):180-95. doi: 10.1111/mmi.12092. Epub 2012 Nov 30. PubMed PMID: 23199363.
    Tseng CL, Chen JT, Lin JH, Huang WZ, Shaw GC

    Genetic evidence for involvement of the alternative sigma factor SigI in controlling expression of the cell wall hydrolase gene lytE and contribution of LytE to heat survival of Bacillus subtilis

    Arch Microbiol. 2011 Sep;193(9):677-85. doi: 10.1007/s00203-011-0710-0. Epub 2011 May 4. PubMed PMID: 21541672.
    Schirner K, Errington J

    The cell wall regulator {sigma}I specifically suppresses the lethal phenotype of mbl mutants in Bacillus subtilis

    J Bacteriol. 2009 Mar;191(5):1404-13. doi: 10.1128/JB.01497-08. Epub 2008 Dec 29. PubMed PMID: 19114499; PubMed Central PMCID: PMC2648184.
    Tseng CL, Shaw GC

    Genetic evidence for the actin homolog gene mreBH and the bacitracin resistance gene bcrC as targets of the alternative sigma factor SigI of Bacillus subtilis

    J Bacteriol. 2008 Mar;190(5):1561-7. Epub 2007 Dec 21. PubMed PMID: 18156261; PubMed Central PMCID: PMC2258693.
    Asai K, Ootsuji T, Obata K, Matsumoto T, Fujita Y, Sadaie Y

    Regulatory role of RsgI in sigI expression in Bacillus subtilis

    Microbiology. 2007 Jan;153(Pt 1):92-101. PubMed PMID: 17185538.
    Zuber U, Drzewiecki K, Hecker M

    Putative sigma factor SigI (YkoZ) of Bacillus subtilis is induced by heat shock

    J Bacteriol. 2001 Feb;183(4):1472-5. PubMed PMID: 11157964; PubMed Central PMCID: PMC95025.
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