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Categories containing this gene/protein


transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons


SigV regulon

The SigV regulon


Gene


  • Coordinates on the chromosome (coding sequence): 2,769,850 -> 2,770,350
  • Phenotypes of a mutant

  • increased sensitivity to lysozyme, this can be suppressed by overexpression of oatA PubMed
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • ECF subfamily (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

  • activity is controlled by the interaction with RsiV, proteolytic degradation of RsiV releases active SigV PubMed
  • Structure

    Localization

    Interactions

  • SigV-RsiV, this interaction inhibits the activity of SigV PubMed
  • SigV-(RpoB-RpoC)
  • Additional information

  • Expression of the SigV regulon in increased in ugtP mutants PubMed
  • Expression and Regulation


    Operon

  • sigV-rsiV-oatA-yrhK PubMed
  • Sigma factor

  • SigV PubMed
  • Regulation

  • induced by lysozyme (SigV) PubMed
  • Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • MGNA-A144 (sigV::erm), available at the NBRP B. subtilis, Japan
  • 1A907 ( sigV::kan), PubMed, available at BGSC
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Thomas Wiegert, University of Bayreuth, Germany Homepage
  • References


    Reviews

    Helmann JD

    Bacillus subtilis extracytoplasmic function (ECF) sigma factors and defense of the cell envelope

    Curr Opin Microbiol. 2016 Apr;30:122-32. doi: 10.1016/j.mib.2016.02.002. Epub 2016 Feb 20. Review. PubMed PMID: 26901131; PubMed Central PMCID: PMC4821709.
    Souza BM, Castro TL, Carvalho RD, Seyffert N, Silva A, Miyoshi A, Azevedo V

    σ(ECF) factors of gram-positive bacteria: a focus on Bacillus subtilis and the CMNR group

    Virulence. 2014 Jul 1;5(5):587-600. doi: 10.4161/viru.29514. Epub 2014 Jun 12. Review. PubMed PMID: 24921931; PubMed Central PMCID: PMC4105308.

    Original publications

    Seki T, Mineshima R, Hashimoto M, Matsumoto K, Hara H, Matsuoka S

    Repression of the activities of two extracytoplasmic function σ factors, σM and σV, of Bacillus subtilis by glucolipids in Escherichia coli cells

    Genes Genet Syst. 2015;90(2):109-14. doi: 10.1266/ggs.90.109. PubMed PMID: 26399770.
    Hastie JL, Williams KB, Ellermeier CD

    The activity of σV, an extracytoplasmic function σ factor of Bacillus subtilis, is controlled by regulated proteolysis of the anti-σ factor RsiV

    J Bacteriol. 2013 Jul;195(14):3135-44. doi: 10.1128/JB.00292-13. Epub 2013 May 17. PubMed PMID: 23687273; PubMed Central PMCID: PMC3697642.
    Matsuoka S, Chiba M, Tanimura Y, Hashimoto M, Hara H, Matsumoto K

    Abnormal morphology of Bacillus subtilis ugtP mutant cells lacking glucolipids

    Genes Genet Syst. 2011;86(5):295-304. PubMed PMID: 22362028.
    Guariglia-Oropeza V, Helmann JD

    Bacillus subtilis σ(V) confers lysozyme resistance by activation of two cell wall modification pathways, peptidoglycan O-acetylation and D-alanylation of teichoic acids

    J Bacteriol. 2011 Nov;193(22):6223-32. doi: 10.1128/JB.06023-11. Epub 2011 Sep 16. PubMed PMID: 21926231; PubMed Central PMCID: PMC3209214.
    Ho TD, Hastie JL, Intile PJ, Ellermeier CD

    The Bacillus subtilis extracytoplasmic function σ factor σ(V) is induced by lysozyme and provides resistance to lysozyme

    J Bacteriol. 2011 Nov;193(22):6215-22. doi: 10.1128/JB.05467-11. Epub 2011 Aug 19. PubMed PMID: 21856855; PubMed Central PMCID: PMC3209206.
    Luo Y, Asai K, Sadaie Y, Helmann JD

    Transcriptomic and phenotypic characterization of a Bacillus subtilis strain without extracytoplasmic function σ factors

    J Bacteriol. 2010 Nov;192(21):5736-45. doi: 10.1128/JB.00826-10. Epub 2010 Sep 3. PubMed PMID: 20817771; PubMed Central PMCID: PMC2953670.
    Hashimoto M, Takahashi H, Hara Y, Hara H, Asai K, Sadaie Y, Matsumoto K

    Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with membranes of reduced phosphatidylglycerol content

    Genes Genet Syst. 2009 Jun;84(3):191-8. PubMed PMID: 19745567.
    Mascher T, Hachmann AB, Helmann JD

    Regulatory overlap and functional redundancy among Bacillus subtilis extracytoplasmic function sigma factors

    J Bacteriol. 2007 Oct;189(19):6919-27. Epub 2007 Aug 3. PubMed PMID: 17675383; PubMed Central PMCID: PMC2045236.
    Zellmeier S, Hofmann C, Thomas S, Wiegert T, Schumann W

    Identification of sigma(V)-dependent genes of Bacillus subtilis

    FEMS Microbiol Lett. 2005 Dec 15;253(2):221-9. Epub 2005 Oct 24. PubMed PMID: 16274938.
    Yoshimura M, Asai K, Sadaie Y, Yoshikawa H

    Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors

    Microbiology. 2004 Mar;150(Pt 3):591-9. PubMed PMID: 14993308.
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