SubtiBank SubtiBank
'

Categories containing this gene/protein


transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons


SigY regulon

The SigY regulon


Gene


  • Coordinates on the chromosome (coding sequence): 3,970,312 -> 3,970,848
  • Phenotypes of a mutant

  • loss of the [[SPß prophage]] PubMed
  • sensitive to killing by sublancin PubMed
  • no production of sublancin PubMed
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • ECF subfamily (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

    Interactions

  • SigY-(RpoB-RpoC)
  • SigY- YxlC PubMed
  • Additional information

    Expression and Regulation


    Operon

  • sigY-yxlC-yxlD-yxlE-yxlF-yxlG PubMed
  • Sigma factor

  • SigY PubMed
  • Regulation

    Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • MGNA-B751 (sigY::erm), available at the NBRP B. subtilis, Japan
  • 1A909 ( sigY::erm), PubMed, available at BGSC
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Reviews

    Souza BM(1), Castro TL(1), Carvalho RD(1), Seyffert N(1), Silva A(2), Miyoshi A(1), Azevedo V(1).

    σ(ECF) factors of gram-positive bacteria: a focus on Bacillus subtilis and the CMNR group.

    Virulence. 2014 Jul 1;5(5):587-600. doi: 10.4161/viru.29514. Epub 2014 Jun 12.

    The SigY regulon

    Mascher T(1), Hachmann AB, Helmann JD.

    Regulatory overlap and functional redundancy among Bacillus subtilis extracytoplasmic function sigma factors.

    J Bacteriol. 2007 Oct;189(19):6919-27. Epub 2007 Aug 3.

    Other original publications

    Guzina J, Djordjevic M

    Promoter Recognition by Extracytoplasmic Function σ Factors: Analyzing DNA and Protein Interaction Motifs

    J Bacteriol. 2016 Jun 27;198(14):1927-38. doi: 10.1128/JB.00244-16. PubMed PMID: 27137497; PubMed Central PMCID: PMC4936102.
    Guzina J, Djordjevic M

    Promoter Recognition by Extracytoplasmic Function σ Factors: Analyzing DNA and Protein Interaction Motifs

    J Bacteriol. 2016 Jun 27;198(14):1927-38. doi: 10.1128/JB.00244-16. PubMed PMID: 27137497; PubMed Central PMCID: PMC4936102.
    Mendez R, Gutierrez A, Reyes J, Márquez-Magaña L

    The extracytoplasmic function sigma factor SigY is important for efficient maintenance of the Spβ prophage that encodes sublancin in Bacillus subtilis

    DNA Cell Biol. 2012 Jun;31(6):946-55. doi: 10.1089/dna.2011.1513. PubMed PMID: 22400495; PubMed Central PMCID: PMC3378957.
    Luo Y, Asai K, Sadaie Y, Helmann JD

    Transcriptomic and phenotypic characterization of a Bacillus subtilis strain without extracytoplasmic function σ factors

    J Bacteriol. 2010 Nov;192(21):5736-45. doi: 10.1128/JB.00826-10. PubMed PMID: 20817771; PubMed Central PMCID: PMC2953670.
    Yoshimura M, Asai K, Sadaie Y, Yoshikawa H

    Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors

    Microbiology. 2004 Mar;150(Pt 3):591-9. PubMed PMID: 14993308.
    Tojo S, Matsunaga M, Matsumoto T, Kang CM, Yamaguchi H, Asai K, Sadaie Y, Yoshida K, Fujita Y

    Organization and expression of the Bacillus subtilis sigY operon

    J Biochem. 2003 Dec;134(6):935-46. PubMed PMID: 14769884.
    Cao M, Salzberg L, Tsai CS, Mascher T, Bonilla C, Wang T, Ye RW, Márquez-Magaña L, Helmann JD

    Regulation of the Bacillus subtilis extracytoplasmic function protein sigma(Y) and its target promoters

    J Bacteriol. 2003 Aug;185(16):4883-90. PubMed PMID: 12897008; PubMed Central PMCID: PMC166484.
    '
    No login