SubtiBank SubtiBank

Categories containing this gene/protein

phosphorelay, phosphoproteins

This gene is a member of the following regulons


  • Coordinates on the chromosome (coding sequence): 2,853,981 -> 2,854,559
  • Phenotypes of a mutant

  • Arrest of sporulation at stage 0 (initiation) PubMed
  • The protein

    Catalyzed reaction/ biological activity

    Protein family

    Paralogous protein(s)

    Kinetic information




    Effectors of protein activity


  • 1F51 (complex with Spo0F), 1IXM
  • Localization


  • Spo0B-Spo0F
  • Spo0B-Spo0A
  • YmcA-Spo0F PubMed
  • Additional information

    Expression and Regulation


  • spo0B-obg-yszB-pheA PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

    Regulatory mechanism

    Additional information

  • expression is repressed by binding of RpoE to the A-rich sequence in the -35 region of the promoter PubMed
  • Biological materials


  • spo0B12, point mutation 85C>T (=R29W), available in BGSC 1S54
  • spo0B136, amber mutation 103A>T (=K35X), available in BGSC 1S16
  • spo0B580ts, point mutation 193G>A (=E65K), available in BGSC 1S90
  • spo0B581ts, point mutation 461G>A (=G154D), available in BGSC 1S91
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct


    Labs working on this gene/protein


    Prajapati RK, Sur R, Mukhopadhyay J

    A Novel Function of ╬┤ Factor from Bacillus subtilis as a Transcriptional Repressor

    J Biol Chem. 2016 Nov 11;291(46):24029-24035. Epub 2016 Sep 26. PubMed PMID: 27679485; PubMed Central PMCID: PMC5104928.
    Carabetta VJ, Tanner AW, Greco TM, Defrancesco M, Cristea IM, Dubnau D

    A complex of YlbF, YmcA and YaaT regulates sporulation, competence and biofilm formation by accelerating the phosphorylation of Spo0A

    Mol Microbiol. 2013 Apr;88(2):283-300. doi: 10.1111/mmi.12186. Epub 2013 Mar 12. PubMed PMID: 23490197; PubMed Central PMCID: PMC3781937.
    Eswaramoorthy P, Dinh J, Duan D, Igoshin OA, Fujita M

    Single-cell measurement of the levels and distributions of the phosphorelay components in a population of sporulating Bacillus subtilis cells

    Microbiology. 2010 Aug;156(Pt 8):2294-304. doi: 10.1099/mic.0.038497-0. Epub 2010 Apr 22. PubMed PMID: 20413551.
    Varughese KI, Tsigelny I, Zhao H

    The crystal structure of beryllofluoride Spo0F in complex with the phosphotransferase Spo0B represents a phosphotransfer pretransition state

    J Bacteriol. 2006 Jul;188(13):4970-7. Erratum in: J Bacteriol. 2007 Apr;189(8):3328. PubMed PMID: 16788205; PubMed Central PMCID: PMC1482986.
    Fujita M, Losick R

    The master regulator for entry into sporulation in Bacillus subtilis becomes a cell-specific transcription factor after asymmetric division

    Genes Dev. 2003 May 1;17(9):1166-74. PubMed PMID: 12730135; PubMed Central PMCID: PMC196045.
    Stephenson SJ, Perego M

    Interaction surface of the Spo0A response regulator with the Spo0E phosphatase

    Mol Microbiol. 2002 Jun;44(6):1455-67. PubMed PMID: 12067336.
    Zapf J, Sen U, Madhusudan, Hoch JA, Varughese KI

    A transient interaction between two phosphorelay proteins trapped in a crystal lattice reveals the mechanism of molecular recognition and phosphotransfer in signal transduction

    Structure. 2000 Aug 15;8(8):851-62. PubMed PMID: 10997904.
    Tzeng YL, Zhou XZ, Hoch JA

    Phosphorylation of the Spo0B response regulator phosphotransferase of the phosphorelay initiating development in Bacillus subtilis

    J Biol Chem. 1998 Sep 11;273(37):23849-55. PubMed PMID: 9726997.
    Grimshaw CE, Huang S, Hanstein CG, Strauch MA, Burbulys D, Wang L, Hoch JA, Whiteley JM

    Synergistic kinetic interactions between components of the phosphorelay controlling sporulation in Bacillus subtilis

    Biochemistry. 1998 Feb 3;37(5):1365-75. PubMed PMID: 9477965.
    Tzeng YL, Hoch JA

    Molecular recognition in signal transduction: the interaction surfaces of the Spo0F response regulator with its cognate phosphorelay proteins revealed by alanine scanning mutagenesis

    J Mol Biol. 1997 Sep 19;272(2):200-12. PubMed PMID: 9299348.
    Zhou XZ, Madhusudan, Whiteley JM, Hoch JA, Varughese KI

    Purification and preliminary crystallographic studies on the sporulation response regulatory phosphotransferase protein, Spo0B, from Bacillus subtilis

    Proteins. 1997 Apr;27(4):597-600. PubMed PMID: 9141138.
    Ferrari FA, Trach K, Hoch JA

    Sequence analysis of the spo0B locus reveals a polycistronic transcription unit

    J Bacteriol. 1985 Feb;161(2):556-62. PubMed PMID: 3918016; PubMed Central PMCID: PMC214918.
    Trach K, Hoch JA

    The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein

    J Bacteriol. 1989 Mar;171(3):1362-71. PubMed PMID: 2537815; PubMed Central PMCID: PMC209754.
    Zuber P, Losick R

    Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis

    J Bacteriol. 1987 May;169(5):2223-30. PubMed PMID: 2437099; PubMed Central PMCID: PMC212136.
    Bai U, Lewandoski M, Dubnau E, Smith I

    Temporal regulation of the Bacillus subtilis early sporulation gene spo0F

    J Bacteriol. 1990 Sep;172(9):5432-9. PubMed PMID: 2118512; PubMed Central PMCID: PMC213209.
    Burbulys D, Trach KA, Hoch JA

    Initiation of sporulation in B

    subtilis is controlled by a multicomponent phosphorelay. Cell. 1991 Feb 8;64(3):545-52. PubMed PMID: 1846779.
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